Herbicides are the foundation of weed control in commercial crop-production systems. However, herbicide-resistant (HR) weed populations are evolving rapidly as a natural response to selection pressure imposed by modern agricultural management activities. Mitigating the evolution of herbicide resistance depends on reducing selection through diversification of weed control techniques, minimizing the spread of resistance genes and genotypes via pollen or propagule dispersal, and eliminating additions of weed seed to the soil seedbank. Effective deployment of such a multifaceted approach will require shifting from the current concept of basing weed management on single-year economic thresholds.

Development of herbicide-resistant crops has resulted in significant changes to agronomic practices, one of which is the adoption of effective, simple, low-risk, crop-production systems with less dependency on tillage and lower energy requirements. Overall, the changes have had a positive environmental effect by reducing soil erosion, the fuel use for tillage, and the number of herbicides with groundwater advisories as well as a slight reduction in the overall environmental impact quotient of herbicide use. However, herbicides exert a high selection pressure on weed populations, and density and diversity of weed communities change over time in response to herbicides and other control practices imposed on them. Repeated and intensive use of herbicides with the same mechanisms of action (MOA; the mechanism in the plant that the herbicide detrimentally affects so that the plant succumbs to the herbicide; e.g., inhibition of an enzyme that is vital to plant growth or the inability of a plant to metabolize the herbicide before it has done damage) can rapidly select for shifts to tolerant, difficult-to-control weeds and the evolution of herbicide-resistant weeds, especially in the absence of the concurrent use of herbicides with different mechanisms of action or the use of mechanical or cultural practices or both.

BACKGROUNDThe widespread occurrence of ALS inhibitor‐ and glyphosate‐resistant Amaranthus palmeri has led to increasing use of protoporphyrinogen oxidase (PPO)‐inhibiting herbicides in cotton and soybean. Studies were conducted to confirm resistance to fomesafen (a PPO inhibitor), determine the resistance frequency, examine the resistance profile to other foliar‐applied herbicides and investigate the resistance mechanism of resistant plants in a population collected in 2011 (AR11‐LAW B) and its progenies from two cycles of fomesafen selection (C1 and C2).RESULTSThe frequency of fomesafen‐resistant plants increased from 5% in the original AR11‐LAW‐B to 17% in the C2 population. The amounts of fomesafen that caused 50% growth reduction were 6‐, 13‐ and 21‐fold greater in AR11‐LAW‐B, C1 and C2 populations, respectively, than in the sensitive ecotype. The AR11‐LAW‐B population was sensitive to atrazine, dicamba, glufosinate, glyphosate and mesotrione but resistant to ALS‐inhibiting herbicides pyrithiobac and trifloxysulfuron. Fomesafen survivors from C1 and C2 populations tested positive for the PPO glycine 210 deletion previously reported in waterhemp (Amaranthus tuberculatus).CONCLUSIONThese studies confirmed that Palmer amaranth in Arkansas has evolved resistance to foliar‐applied PPO‐inhibiting herbicide. © 2016 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Changes in the weed flora of cropping systems reflect the impacts of factors that create safe sites for weed establishment and facilitate the influx and losses to and from the soil seedbank. This analysis of the annual surveys of the Southern Weed Science Society documents changes in the weed flora of the 14 contiguous southern states since the advent of transgenic, herbicide-resistant crops. In 1994 and 2009, the top five weeds in corn were morningglories, Texas millet, broadleaf signalgrass, johnsongrass, and sicklepod; in this same period Palmer amaranth, smartweeds, and goosegrass had the greatest increases in importance in corn. In cotton, morningglories and nutsedges were among the top five most troublesome weeds in 1995 and 2009. Palmer amaranth, pigweeds, and Florida pusley were also among the five most troublesome species in 2009; the weeds with the largest increases in importance in cotton were common ragweed and two species with tolerance to glyphosate, Benghal dayflower and Florida pusley. In soybean, morningglories, nutsedges, and sicklepod were among the top five weed species in 1995 and 2009. Two species with glyphosate resistance, Palmer amaranth and horseweed, were the second and fourth most troublesome weeds of soybean in 2009. In wheat, the top four weeds in 2008 were the same as those in 1994 and included Italian ryegrass, wild garlic, wild radish, and henbit. Crop production in the southern region is a mosaic of various crop rotations, soil types, and types of tillage. During the interval between the surveys, the predominant change in weed management practices in the region and the nation was the onset and rapid dominance of the use of glyphosate in herbicide-resistant cultivars of corn, cotton, and soybean. Because of the correspondence between the effects of glyphosate on the respective weed species and the observed changes in the weed flora of the crops, it is likely the very broad use of glyphosate was a key component shaping the changes in weed flora. Only eight of the top 15 most troublesome weeds of cotton and soybean, the crops with the greatest use of glyphosate, were the same in 1995 and 2009. In contrast, in corn and wheat where adoption of glyphosate-resistant cultivars lags or is absent, 12 of the 15 most troublesome weeds were the same in 1994 and 2008. These findings show on a regional scale that weeds adapt to recurrent selection from herbicides, currently the predominant weed management tool. Future research should seek methods to hinder the rapid spread of herbicide-tolerant and evolution of herbicide-resistant weed species. As new tools are developed, research should focus on ways to preserve the efficacy of those tools through improved stewardship.

Diseases such as Fusarium wilt [Fusarium oxysporum f.sp. vasinfectum (FOV) Atk. Sny & Hans] represent expanding threats to cotton production. Integrating disease resistance into high-yielding, high-fiber quality cotton (Gossypium spp.) cultivars is one of the most important objectives in cotton breeding programs worldwide. In this study, we conducted a comprehensive analysis of gene action in cotton governing FOV race 4 resistance by combining conventional inheritance and quantitative trait loci (QTL) mapping with molecular markers. A set of diverse cotton populations was generated from crosses encompassing multiple genetic backgrounds. FOV race 4 resistance was investigated using seven parents and their derived populations: three intraspecific (G. hirsutum × G. hirsutum L. and G. barbadense × G. barbadense L.) F1 and F2; five interspecific (G. hirsutum × G. barbadense) F1 and F2; and one RIL. Parents and populations were evaluated for disease severity index (DSI) of leaves, and vascular stem and root staining (VRS) in four greenhouse and two field experiments. Initially, a single resistance gene (Fov4) model was observed in F2 populations based on inheritance of phenotypes. This single Fov4 gene had a major dominant gene action and conferred resistance to FOV race 4 in Pima-S6. The Fov4 gene appears to be located near a genome region on chromosome 14 marked with a QTL Fov4-C14 1 , which made the biggest contribution to the FOV race 4 resistance of the generated F2 progeny. Additional genetic and QTL analyses also identified a set of 11 SSR markers that indicated the involvement of more than one gene and gene interactions across six linkage groups/chromosomes (3, 6, 8, 14, 17, and 25) in the inheritance of FOV race 4 resistance. QTLs detected with minor effects in these populations explained 5-19 % of the DSI or VRS variation. Identified SSR markers for the resistance QTLs with major and minor effects will facilitate for the first time marker-assisted selection for the introgression of FOV race 4 resistance into elite cultivars during the breeding process.

A highly virulent cotton wilt pathogen, Fusarium oxysporum f. sp. vasinfectum VCG0114 (race 4) was found in West Texas in 2017, after being known in California since 2001. Isolates obtained from wilted plants collected in 2017 from Texas, in 2015 from China, and during 2001 to 2014 from California and isolates from historical collections including the race 4 reference isolate were characterized by soil-infestation pathogenicity assays, DNA sequence analysis, and vegetative compatibility analysis. All obtained F. oxysporum f. sp. vasinfectum isolates belonged to VCG0114. All of these isolates, except one isolate from China, caused disease in a soil-infestation assay without nematodes. Thus, they belong to the nematode-independent pathotype. Texas isolates were significantly more virulent than were isolates from China or California on Gossypium barbadense ‘Pima S-7’. Four different genotypes (N, T, MT, and MiT) were identified based on the transposable element Tfo1 insertion into the PHO gene and independent MULE or MITE insertions into the Tfo1 transposon. Some significant differences in virulence were detected among the genotypes in some locations. No differences in pathogenicity were observed between the California and China collection isolates on Pima S-7, and the virulence of the major genotypes was similar on the Gossypium hirsutum cultivar ‘Stoneville 474’ or the Barbren 713 germplasm line. Simple polymerase chain reaction (PCR) methods were developed to specifically determine and detect the four genotypes within VCG0114. A specific PCR method to detect all VCG0114 isolates was also developed. These methods will facilitate the timely identification of infested fields and seed lots and the elucidation of evolutionary relationships among the isolates. This should help to closely monitor the movement of the pathogen and reduce dissemination of these devastating pathogens.

absence of late season insect pressure, however, poorly drained soils and a history of inclement weather patterns Full season cotton (Gossypium hirsutum L.) cultivars may be better during the fall months continue to necessitate manageadapted to the lower southeastern USA because early maturing cultivars may not recover from the many episodic drought events that ment for early crop maturity in some regions of the U.S. annually plague the region. The objective of this investigation was to Cotton Belt. In the lower southeastern USA, however, determine if cotton maturity may be defined on the basis of flowering more favorable fall weather conditions and well drained interval, boll maturation period, or whole plant yield distribution. soils may not necessitate management for early crop Studies were conducted at the University of Georgia Coastal Plain maturity. In fact, management for full season crop matu-Experiment Station in 2001, 2002, and 2003. Nine commercially availrity may be the more suitable approach. able cotton cultivars were over seeded and hand thinned to 10.8 plants Water availability is frequently the most limiting facm Ϫ2 . Areas within each plot were reserved for daily white flower and tor to profitable cotton production in the southeastern open boll tagging and hand harvest. Mean vertical flowering intervals USA. Because of the shallow, coarse textured soils of were 2.1 (2001), 2.7 (2002), and 2.6 (2003) d. Mean vertical flowering intervals increased from main stem node 5 to about main stem node the Coastal Plain and the unreliable rainfall patterns 11 and then began to decline. Mean horizontal flowering intervals

A strain of the whitefly Bemisia tabaci (Gennadius) possessing unusually high levels of resistance to a wide range of insecticides was discovered in 2004 in the course of routine resistance monitoring in Arizona. The multiply resistant insects, collected from poinsettia (Euphorbia pulcherrima Willd. ex Klotzsch) plants purchased at a retail store in Tucson, were subjected to biotype analysis in three laboratories. Polyacrylamide gel electrophoresis of naphthyl esterases and sequencing of the mitochondrial cytochrome oxidase I gene (780 bp) confirmed the first detection of the Q biotype of B. tabaci in the New World. This U.S. Q biotype strain, referred to as Poinsettia'04, was highly resistant to two selective insect growth regulators, pyriproxyfen and buprofezin, and to mixtures of fenpropathrin and acephate. It was also unusually low in susceptibility to the neonicotinoid insecticides imidacloprid, acetamiprid, and thiamethoxam, relative to B biotype whiteflies. In 100 collections of whiteflies made in Arizona cotton (Gossypium spp.), vegetable, and melon (Cucumis melo L.) fields from 2001 to 2005, no Q biotypes were detected. Regions of the United States that were severely impacted by the introduction of the B biotype of B. tabaci in the 1980s would be well advised to promote measures that limit movement of the Q biotype from controlled environments into field systems and to formulate alternatives for managing this multiply-resistant biotype, in the event that it becomes more widely distributed.