Cell volume variation under different concentrations of saline solution (NaCL)

García, Mario J.; Ardila, A. M.

doi:10.1016/s0120-3347(09)72002-7

Cited by 5 publications

(4 citation statements)

References 4 publications

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“…To further analyze the effects of the carbon dots addition, spectra with an increasing concentration of NaCl in the matrix/analyte mixture were acquired. Even though, 0.9% of NaCl is considered as relevant and closest to isotonic, in our approach we applied the concentrations up to 2% of NaCl [61], to test the limits for cholesterol detection. The spectra were acquired with sDHB, DHB, CDs and N-CDs (alone, or in a combination with the organic matrix).…”

Section: Maldi-tof Ms Of Cholesterol With N-cds and Cds With Inorgani...mentioning

confidence: 99%

Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry

Houdová

Soto

Castro

et al. 2021

Journal of Colloid and Interface Science

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Section: Maldi-tof Ms Of Cholesterol With N-cds and Cds With Inorgani...mentioning

confidence: 99%

Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry

Houdová

Soto

Castro

et al. 2021

Journal of Colloid and Interface Science

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“…2. Typical histograms of mammalian erythrocyte distribution: a -human, b -rabbit, c -rat тин внаслідок виходу води [1,5]. З іншого боку, відомо [6,8,19,13], що в гіпертонічних розчинах змінюються морфологічні характеристики клітин; це теж має вплив на значення медіани розподілу.…”

Section: результати й обговоренняunclassified

The role of the dehydration stage in the post-hypertonic hemolysis of mammalian erythrocytes

Nipot¹,

Shapkina²,

Zubov³

et al. 2021

Bìol. Tvarin

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The aim of this study was to assess the level of damage to mammalian erythrocytes under post-hypertonic shock depending on the concentration of NaCl in the dehydration medium and to determine the effect of hypertonic NaCl solutions on the condition of mammalian erythrocytes by flow cytometry. To achieve this goal, spectrophotometric and cytometry research methods were used. The data obtained showed that post-hypertonic lysis of mammalian erythrocytes depends on the concentration of NaCl in the dehydration medium. The most sensitive to the effects of post-hypertonic shock are rat erythrocytes, the least sensitive are rabbit cells. Cytometry studies revealed significant changes in the histograms of the distribution of erythrocytes of all mammalian species with increasing salt concentration in the dehydration medium. These changes are species-specific and are probably related to changes in cell volume and morphology. The data revealed a relationship between the level of post-hypertonic hemolysis and the values of such indicators as the median distribution and the coefficient of variation. Thus, an increase in the sensitivity of mammalian erythrocytes to post-hypertonic shock with increasing salt concentration in dehydration medium was usually accompanied by a decrease in the median cell division, and higher values of the coefficient of variation are characteristic of mammalian erythrocytes resistant to post-hypertonic shock.

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“…Se define una solución isotónica cuando el líquido intracelular (LIC), posee la misma concentración de solutos que el líquido extracelular (LEC), por lo que el volumen celular permanece igual y no existe efecto alguno sobre la presión osmótica de las células [5,6]. Son empleadas en la aplicación de medicamentos por vía intravenosa y en la perdida de líquidos como la deshidratación o hemorragias, recuperando así el volumen del comportamiento intravascular [2].…”

Section: Introductionunclassified

Identificación de bacterias presentes en tres soluciones intravenosas en un periodo mayor a 72 horas

Batallas-Canchig,

Castillo-Hidalgo,

Gancino–Carvajal

2023

RC FCV-LUZ

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Las soluciones intravenosas son empleadas en la práctica diaria en Medicina Veterinaria para tratamientos médicos como mantenimiento y/o reposición de fluidos y la administración de medicamentos, pero al ser reutilizadas por periodos largos de tiempo, el riesgo de contaminación bacteriana aumenta. El objetivo principal de esta investigación fue la evaluación de la tasa de contaminación de tres soluciones intravenosas cristaloides, durante un periodo mayor a 72 h en situaciones clínicas habituales del ambiente veterinario. Las soluciones fueron analizas a las 96; 120 y 144 h, se determinó que, a partir de las 96 h de utilización de las soluciones intravenosas, ya existe contaminación bacteriana hasta en un 100 % como es el caso de la dextrosa al 50 %, y una contaminación del 75 % a las 120 h de la solución de cloruro de sodio al 0,9 %, y hasta un 50 % de contaminación de la solución de lactato de Ringer a las 144 h. Se concluye que partir de las 96 h de manipular las soluciones intravenosas, ya existe la presencia de bacterias ambientales, sobre todo en las soluciones dextrosadas que son las más propensas a la contaminación, a pesar de aplicar las técnicas antisépticas adecuadas.

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Cell volume variation under different concentrations of saline solution (NaCL)

Cited by 5 publications

References 4 publications

Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry

Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry

The role of the dehydration stage in the post-hypertonic hemolysis of mammalian erythrocytes

Identificación de bacterias presentes en tres soluciones intravenosas en un periodo mayor a 72 horas

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