Cell types in the peripheral blood of an air‐breathing fish Channa punctatus

Abstract

Different cell types found in the peripheral blood of Cknna punctatus, an air-breathing freshwater fish have been characterized and identified using morphological, morphometric, cytochemical and autoradiographic techniques. Some of the cytochemical methods used, particularly that of differential staining of haemoglobin have been developed and used for the first time in fishes.

Blood and other possible inflammatory cells in the sparid Acanthopagrus australis (Günther)

“…With regard to the bream monocyte, previous descriptions of teleost monocytes in Romanowsky-stained blood smears are of a large cell with pseudopodia, a pale to dark and often vacuolated basophilic cytoplasm, and a nucleus that is often irregular in outline, rounded or indented and finely reticular (Ellis, 1976;Grizzle & Rogers, 1976;Williams & Warner, 1976;Mahajan & Dheer, 1979;Barber et al, 1981;Elarifi, 1982;Groman, 1982;Yasutake & Wales, 1983). Furthermore, the ultrastructural features of the monocyte of the bream (large Golgi complex with numerous vesicles, few small granules, pseudopodia, rER and vacuoles) resemble those given for the monocytes of other teleosts (Ferguson, 1976;Kreutzmann, 1977;Bielek, 1980b;Hawkins et al, 1981;Cenini, 1984).…”

“…Furthermore, the ultrastructural features of the monocyte of the bream (large Golgi complex with numerous vesicles, few small granules, pseudopodia, rER and vacuoles) resemble those given for the monocytes of other teleosts (Ferguson, 1976;Kreutzmann, 1977;Bielek, 1980b;Hawkins et al, 1981;Cenini, 1984). LYMPHOCYTE Although large and small lymphocytes have been described in teleosts by a number of authors (e.g., Klontz, 1972;Hines & Spira, 1973;Christensen et al, 1978;Lewis et af., 1979;Mahajan & Dheer, 1979), some authors (Williams & Warner, 1976;Elarifi, 1982), as well as myself, have only recognized one type of lymphocyte with a large size-range. Ellis (1977) points out that the distinction between large and small lymphocytes may be arbitrary as they probably represent different stages of the same cell type, but immunological evidence for two types of lymphocytes in the channel catfish, Ictalurus punctatus, has been provided by Lewis et al (1979), and an extensive discussion given by Ellis (1982).…”

Studies on classification of eel leucocytes.

“…[16][17][18] In this study, phagocytosis by thrombocytes was observed, however from their weakness of phagocytosis, they must have other properties. As for the function of neutrophils and monocytes, Ferguson13) revealed the different degree of phagocytosis by neutrophils and monocytes.…”

Differential leucokocyte counts in ''dourado'', Salminus maxillosus Valenciennes, 1840, from the Mogi-Guaçu River, Pirassununga, SP

“…This change is also possible due to fish sensitivity to variation in temperature, more than to a compensatory immune mechanism (Quentel & Obach, 1992). Increases in neutrophil percentage is generally associated with some pathology in fish, as reported for an infection by Ichithyophthirius multifilis (Hines & Spira, 1973), a viral infection in Oncorhynchus nerka (Watson et al, 1956), an infection by digenetic parasites in Channa punctatus (Mahajan & Dheer, 1979), and an infection by ectoparasites during the reproductive period in adult specimens of Salmo trutta (Pickering, 1986). When the percentages of leukocytes are analyzed in relation to the biological data of total weight and total length (Table 3), it is noted that the "r" values are rather low with shows that these percentages do not change with fish growning.…”

“…Nonetheless, the influence of environmental variation on the hematologic picture has demonstrated that exogenous factors such as temperature, pressure, salinity, pH, and dissolved O 2 , among others, can alter blood values (Mahajan & Dheer, 1979). This variation in blood composition is observed, not only among different species, but also in individuals of the same species or even in a single individual, depending on the environmental and physiological conditions to which fish are subjected (Kalashnikova, 1976).…”