Cell Toxicity Studies of Albumin-Based Nanosized Magnetic Beads

Abstract

The aim of this study was to prepare bovine serum albumin-based beads containing maghemite nanoparticles incorporated via ionic magnetic fluid and to evaluate the cell toxicity of this biocompatible system using the J774-A1 cell line. Transmission electron micrographs obtained from the magnetic fluid sample were used to estimate the average particle diameter around 7.6 nm and diameter dispersion of 0.22. The BSA-based magnetic beads were prepared using the heat protein denaturation route. The nanoparticle concentration in the magnetic fluid sample used for the synthesis of the magnetic beads was in the range of 1.2 x 10(16) to 2.3 x 10(17) particle/ml. The methodology used to investigate the cell toxicity of the magnetic beads was the classical MTT assay. Our observation showed that the toxicity against the J774-A1 cell line depends upon the amount of magnetic material incorporated into the magnetic nanobeads and was found to be 14, 11, 9, 5, and 3% for 2.3 x 10(17), 1.2 x 10(17), 4.6 x 10(16), 2.3 x 10(16), and 1.2 x 10(16) particle/ml, respectively.

Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system

“…17 MAN was prepared following the heat denaturation method at 100°C of an aqueous solution containing bovine serum albumin (BSA) under mechanical stirring at 13,000 rpm for 20 minutes. Then an aliquot of the ionic magnetic fluid sample containing maghemite nanoparticles was added to the BSA aqueous solution.…”

Mössbauer spectroscopy study of iron oxide nanoparticles obtained by spray pyrolysis

“…As the interest in magnetic nanoparticle (NP) for biomedical applications has been dramatically increased lately, their precise characterizations in terms of magnetic properties, crystal structure, particle size, and bio-compatibility are being urgently required to support the technical specifications [1][2][3]. Among the biomedical applications of magnetic NP in vitro diagnostic by magnetic separation and selection appear to be an important research field.…”

Nanoscaled biocompatible magnetic drug-delivery system: preparation and characterization

“…Magnetic separation techniques have been successfully employed in sorting cells and biologically active molecules [1][2][3] whereas site-specific magnetohyperthermia has been introduced as a promising cancer therapy [4][5][6]. These applications deal with nanosized magnetic particles either surface-coated with biologically friendly molecules while suspended in physiological medium [7][8][9] or supported in biocompatible hosting templates [10][11][12]. Among the cubic ferrite materials maghemite [13] and magnetite [14] nanoparticles are the most widely used materials though cobalt ferrite has been proved sufficiently biocompatible as a material platform for biomedical and biological applications [15].…”

“…Among the cubic ferrite materials maghemite [13] and magnetite [14] nanoparticles are the most widely used materials though cobalt ferrite has been proved sufficiently biocompatible as a material platform for biomedical and biological applications [15]. A wide variety of biocompatible templates can be used to host magnetic nanoparticles, with special emphasis on maghemite introduced in the hosting template via pre-synthesized magnetic fluid (MF) samples [12]. As long as the end magnetic nanocomposite offers long term biocompatibility and chemical stability a wide range of applications opens up for this material platform [16,17].…”