Cell Therapy with Adipose Tissue-Derived Stem/Stromal Cells for Elastase-Induced Pulmonary Emphysema in Rats

Abstract

Not only HGF, but also CINC-1, secreted from transplanted and viable ASCs presumably contributed to lung repair through angiogenesis.

Intravenous infusion of adipose-derived stem/stromal cells improves functional recovery of rats with spinal cord injury

“…ASCs secrete angiogenic growth factors such as HGF and VEGF [16], which have already been reported to contribute to functional recovery and tissue repair after SCI through angiogenetic and antiapoptotic activity [24,25]. Our study also showed that ASCs secreted abundant growth factors after pre-incubation with bFGF in vitro.…”

“…However, the intravenous infusion of ASCs into SCI rats produced little increase in these growth factors in the injured spinal cords. Systemic transplantation of ASCs in rats with elastase-induced pulmonary emphysema enhanced the HGF expression in the lung tissue [16]. Indeed, we found that ASCs accumulated in the lungs at a high level, perhaps accounting for the high HGF levels observed in the pulmonary emphysema model, but not in the SCI model.…”

“…ASCs and BM-MSCs have similar characteristics [12] and can undergo multilineage differentiation into fat, bone, cartilage, smooth muscle, and neuronal, endothelial or hepatic cells [9,[13][14][15]. ASCs also secrete a variety of growth factors, such as hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF), which might mediate regeneration of the injured spinal cord [16]. Furthermore, adipose tissue contains larger populations of MSCs than does BM [17], thus making ASCs a promising target for regenerative medicine.…”

Animal Models of Chronic Obstructive Pulmonary Disease Exacerbations: A Review of the Current Status

“…In one study of C57BL/6 mice, intratracheal elastase administration produced a greater amount of low-density areas than CS exposure, as observed on quantitative micro-computed tomography [70]. A wide variety of studies have highlighted the proteolytic activity of elastase in causing structural changes, such as higher mean linear intercept and alveolar enlargement both in mice [71][72][73][74][75] and in rats [76][77][78][79] (Table 2). Furthermore, several studies reported changes in ECM composition after elastase administration, such as disorganized elastin [80,81], degradation of proteoglycans [82], and abnormal collagen remodeling [83][84][85][86][87][88].…”

“…Reduced body mass, time-dependent decrease in muscle mass, type I oxidative fibers, and muscle cross-sectional area Braber et al, 2010 [62] A/J mice Animals exposed to CS 5 days/ week for 20 weeks Right ventricle hypertrophy [77] Wistar rats Single intratracheal injection of elastase Alveolar enlargement, reduced arterial oxygen tension Bianchi et al, 2015 [78] Boiati et al, 2010 [79] Wistar rats Single intratracheal injection of elastase Higher mean linear intercept Table 2 Models of emphysema induced by elastase.…”