Intravenous infusion of adipose-derived stem/stromal cells improves functional recovery of rats with spinal cord injury

Ohta, Yuki; Hamaguchi, Akemi; Ootaki, Masanori; Watanabe, Minoru; Takeba, Yuko; Iiri, Taroh; Matsumoto, Naoki; Takenaga, Mitsuko

doi:10.1016/j.jcyt.2017.04.002

Cited by 40 publications

(31 citation statements)

References 35 publications

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“…Transplantation of various cells after SCI has led to increase in GDNF mRNA expression [58][59][60]. GDNF increases survival of motor and sensory neurons, improves motor function, induces neurogenesis and axonal growth, enhances myelination, and alleviates pain (analgesia effect) [35,[58][59][60][61][62].…”

Section: Discussionmentioning

confidence: 99%

Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

et al. 2019

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Background: Evidence has suggested that human adipose-derived stem cells (hADSCs) and low-level laser has neuroprotective effects on spinal cord injury (SCI). Therefore, the combined effect of the hADSCs and laser on neuregeneration and neuropathic pain after SCI was investigated. Methods: Forty-eight adult male Wistar rats with 200-250 g weight were used. Thirty minutes after compression, injury with laser was irritated, and 1 week following SCI, about 1 × 10 6 cells were transplanted into the spinal cord. Motor function and neuropathic pain were assessed weekly. Molecular and histological studies were done at the end of the fourth week. Results: The combined application of hADSCs and laser has significantly improved motor function recovery (p = 0.0001), hyperalgesia (p < 0.05), and allodynia (p < 0.05). GDNF mRNA expression was significantly increased in hADSCs and laser+hADSC-treated animals (p < 0.001). Finally, co-administration of hADSCs and laser has enhanced the number of axons around cavity more than other treatments (p < 0.001). Conclusions: The results showed that the combination of laser and ADSCs could significantly improve the motor function and alleviate SCI-induced allodynia and hyperalgesia. Therefore, using a combination of laser and hADSCs in future experimental and translational clinical studies is suggested.

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Section: Discussionmentioning

confidence: 99%

Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

et al. 2019

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“…14,71 Functional experiments indicate that intravenous application of AD-MSCs improve hindlimb motor function through activation of angiogenesis along with upregulation of upstream kinase protein activity, such as ERK1/2 and Akt, in turn promoting cellular survival pathways and tissue-repair mechanisms. 42 However, while AD-MSC transplantation has been evaluated in animal SCI models, there remains a paucity of large, longitudinal clinical trials utilizing stem cells derived from adipose tissue. Early studies examining the safety of intravenous injection of AD-MSCs reported no adverse side effects, including no observed tumorigenicity.…”

Section: Ad-mscsmentioning

confidence: 99%

Stem cell therapies for acute spinal cord injury in humans: a review

Jin¹,

Medress²,

Azad³

et al. 2019

Neurosurgical Focus

118

102

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Recent advances in stem cell biology present significant opportunities to advance clinical applications of stem cell–based therapies for spinal cord injury (SCI). In this review, the authors critically analyze the basic science and translational evidence that supports the use of various stem cell sources, including induced pluripotent stem cells, oligodendrocyte precursor cells, and mesenchymal stem cells. They subsequently explore recent advances in stem cell biology and discuss ongoing clinical translation efforts, including combinatorial strategies utilizing scaffolds, biogels, and growth factors to augment stem cell survival, function, and engraftment. Finally, the authors discuss the evolution of stem cell therapies for SCI by providing an overview of completed (n = 18) and ongoing (n = 9) clinical trials.

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“…ADMSCs have a wide range of sources, and are easily acquired and cultured without ethical issues [25]. ADMSCs and BMSCs have similar properties and play important roles in promoting angiogenesis, wound healing, reducing inflammatory response and activating lymphocyte proliferation, and thus more and more clinical studies has applied ADMSCs transplantation to treating SCI.…”

Section: Adipose-derived Mesenchymal Stem Cells (Admscs)mentioning

confidence: 99%

“…ADMSCs and BMSCs have similar properties and play important roles in promoting angiogenesis, wound healing, reducing inflammatory response and activating lymphocyte proliferation, and thus more and more clinical studies has applied ADMSCs transplantation to treating SCI. ADMSCs accumulates in the injured spinal cord through vein grafting, then activate cytokines and accelerate the recovery of motor function and tissue repair in rats [25]. Hur et al [26] performed intrathecal autologous transplantation of ADMSCs in 14 patients with SCI, no serious adverse events were found during 8 months of follow-up, and some patients exhibited slight neurological improvement.…”

Section: Adipose-derived Mesenchymal Stem Cells (Admscs)mentioning

confidence: 99%

The cell repair research of spinal cord injury: a review of cell transplantation to treat spinal cord injury

Zhang

Wang

Song

2019

Journal of Neurorestoratology

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Through retrospective analysis of the literature on the cell repair of spinal cord injury worldwide, it is found that the mechanism of cell transplantation repairing spinal cord injury is mainly to replace damaged neurons, protect host neurons, prevent apoptosis, promote axonal regeneration and synapse formation, promote myelination, and secrete trophic factors or growth factors to improve microenvironment. A variety of cells are used to repair spinal cord injury. Stem cells include multipotent stem cells, embryonic stem cells, and induced pluripotent stem cells. The multipotent stem cells are mainly various types of mesenchymal stem cells and neural stem cells. Non-stem cells include olfactory ensheathing cells and Schwann cells. Transplantation of inhibitory interneurons to alleviate neuropathic pain in patients is receiving widespread attention. Different types of cell transplantation have their own advantages and disadvantages, and multiple cell transplantation may be more helpful to the patient’s functional recovery. These cells have certain effects on the recovery of neurological function and the improvement of complications, but further exploration is needed in clinical application. The application of a variety of cell transplantation, gene technology, bioengineering and other technologies has made the prospect of cell transplantation more extensive. There is a need to find a safe and effective comprehensive treatment to maximize and restore the patient’s performance.

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Intravenous infusion of adipose-derived stem/stromal cells improves functional recovery of rats with spinal cord injury

Abstract: Our findings indicated that intravenously transplanted ASCs gradually accumulated in the injured spinal cord, where cytokines such as CINC-1 activated ERK1/2 and Akt, leading to functional recovery.

Cited by 40 publications

References 35 publications

Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

Stem cell therapies for acute spinal cord injury in humans: a review

The cell repair research of spinal cord injury: a review of cell transplantation to treat spinal cord injury

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