Cell suspension culture: A potential in vitro culture method for clonal propagation of coconut plantlets via somatic embryogenesis

Kong, Eveline Yee Yan; Biddle, Julianne; Foale, M. A.; Adkins, S. W.

doi:10.1016/j.indcrop.2020.112125

Cited by 25 publications

(19 citation statements)

References 81 publications

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“…The high demand for coconut (Cocos nucifera L.) seedlings necessitates clonal propagation of elite materials [19] which could be increased by the large potential of in vitro tissue culture. This process is currently used for the safe exchange of coconut palm germoplasma between countries through the use of zygotic embryos [20,21,12].…”

Section: Discussionmentioning

confidence: 99%

Effects of Surface Sterilization Protocol and Haustorium Removal on Germination and Growth of Hybrid PB121 Coconut Palm (Cocos nucifera L.) Zygotic Embryos Cultured in vitro

Agbidinoukoun

Somakpe

Houédjissin

et al. 2020

BJI

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Aims: This study aims to identify the best surface sterilization and evaluate the effect of haustorium suppression on in vitro germination of coconut palm (Cocos nucifera L.) zygotic embryos. Study Design: Survival rate and contamination rate of zygotic embryos after different surface sterilization treatments, regeneration rate and organogenesis through the number of leaves and the length of shoots after haustorium suppression were determined. For data processing, the Analysis of Variance was used to compare the means which were separated according to Tukey test (P = 0.05). Place and Duration of Study: Coconut fruits (hybrid PB121) were collected 12 to 14 months after controlled pollination from CRAPP (Centre de Recherches Agricoles Plantes Pérennes), station of Sèmè-kpodji in Benin. Experiments were done in Central Laboratory of Plant Biotechnology and Plant Improvement, University of Abomey-Calavi and conducted from june to december in 2019. Methodology: For the zygotic embryos surface sterilization, four treatments combining three concentrations (3%, 6% and 15%) of commercial bleach (Javel la Croix© containing 12° active chlorine) and immersion durations (5 min, 10 min and 20 min) were tested and the survival rate were determined for each treatment after two months culture. The zygotic embryos were then divided in two sets (haustorium excised embryos set and the whole embryos set) and cultured in modified Y3 medium supplemented with 7 g L-1 agar, 2.5 g L-1 activated charcoal, 5% sucrose, 6.10-3 mM 2.4 D (2.4-dichlorophonoxyacetic acid), gibberellic acid and 0.3 mM BAP(6-benzylaminopurine). After five months culture, the regeneration rate, the number of leaves and the length of shouts were recorded. Results: The high survival rate (80%) was obtained with 6% of bleach and 20 min for the immersion duration without pre-disinfection. The suppression of haustorium have significantly increased the number of leaves (4.3 ± 0.02) and the length of shoots (16.2 ±0.7cm) compared to the whole zygotic embryos. Conclusion: This protocol can help to ensure better surface sterilization of zygotic embryos before their in vitro culture and the development of vigorous plantlets in order to improve the slow growth of plantlets, when transferred to the greenhouse or field.

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Section: Discussionmentioning

confidence: 99%

Effects of Surface Sterilization Protocol and Haustorium Removal on Germination and Growth of Hybrid PB121 Coconut Palm (Cocos nucifera L.) Zygotic Embryos Cultured in vitro

Agbidinoukoun

Somakpe

Houédjissin

et al. 2020

BJI

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“…Somatic embryogenesis is an alternative, vegetative method of clonal multiplication used in many seed-grown plants that are difficult to propagate, as well as endangered species and those with medicinal properties or are otherwise useful [ 12 , 32 , 33 ]. As plant regeneration by somatic embryogenesis is an asexual process that only involves mitotic cell division, the expected result is to obtain genetically uniform plants.…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of the Genetic and Biochemical Variability of Polyscias filicifolia (Araliaceae) Regenerants Obtained by Indirect and Direct Somatic Embryogenesis as a Source of Triterpenes

Śliwińska

Białek

Orłowska

et al. 2021

IJMS

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Polyscias filicifolia (Araliaceae) is broadly used in traditional medicine in Southeast Asia due to its antimicrobial, immunomodulating and cytotoxic activities. The main groups of compounds responsible for pharmacological effects are believed to be oleanolic triterpene saponins. However, Polyscias plants demonstrate relatively slow growth in natural conditions, which led to applying a developing sustainable source of plant material via primary (PSE), secondary (DSE) and direct somatic embryogenesis from DSE (TSE). The AFLP and metAFLP genotyping resulted in 1277 markers, amplified by a total of 24 pairs of selective primers. Only 3.13% of the markers were polymorphic. The analysis of variance showed that the PSE and TSE regenerants differed only in terms of root number, while the DSE plantlets differed for all studied morphological characteristics. Further, the chemical analysis revealed that oleanolic acid (439.72 µg/g DW), ursolic acid (111.85 µg/g DW) and hederagenin (19.07 µg/g DW) were determined in TSE regenerants. Our results indicate that direct somatic embryogenesis ensures the production of homogeneous plant material, which can serve as a potential source of triterpene compounds. Plants obtained via somatic embryogenesis could also be reintroduced into the natural environment to protect and preserve its biodiversity.

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“…However, the application of a temporary immersion system, where the explants are immersed in a liquid medium for a specific time and duration per day, may improve growth of germinating somatic embryos, providing the benefits of both solid and liquid medium systems [65], and dramatically increasing the SE-generated plantlet rate for coconut. A liquid medium was used to produce viable cell suspension cultures in coconut; however, they were unable to progress to the next stage because it is challenging to produce friable embryogenic calli for SE initiation [47,71]. Table 1 summarizes the in vitro studies undertaken on media and media composition for the induction of callus in coconut.…”

Section: Effect Of Media Compositionmentioning

confidence: 99%

“…For coconut SE, embryogenic callus induction and proliferation can be promoted by a high concentration of auxin (2,4-D at 24 to 600 µM in the presence of AC), whereas a subsequent reduction in 2,4-D concentration has been shown to aid the maturation and germination of the somatic embryos formed [13]. Auxin (2,4-D) has also been used for the initiation of embryogenic cell suspension cultures in coconut propagated through the typical SE pathway [71], but only when provided at a very low concentration (4.5 µM) [47]. The concentration of 2,4-D required for callus induction varies between coconut varieties and for different explant types.…”

Section: Role Of Plant Growth Regulatorsmentioning

confidence: 99%

“…The combination of ABA (10 µM) with amino acids such as glutamine (670 µM) has been shown to improve the production of somatic embryos in date palm (Phoenix dactylifera L.) and the accumulation of storage proteins [77], which may also be possible if applied to coconut. Although AC is a common additive to coconut SE media, mainly to reduce tissue necrosis [13], it has been demonstrated to reduce the effective concentration of PGRs in the media, due to its adsorptive properties [71]. Therefore, it is crucial to understand the full impact of using AC in a coconut tissue culture media before drawing any conclusions concerning the role a particular PGR may play in the somatic embryogenesis of coconut.…”

Section: Role Of Plant Growth Regulatorsmentioning

confidence: 99%

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Cloning Coconut via Somatic Embryogenesis: A Review of the Current Status and Future Prospects

Kalaipandian

Kong

et al. 2021

Plants

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Coconut [Cocos nucifera L.] is often called “the tree of life” because of its many uses in the food, beverage, medicinal, and cosmetic industries. Currently, more than 50% of the palms grown throughout the world are senile and need to be replanted immediately to ensure production levels meet the present and increasing demand for coconut products. Mass replanting will not be possible using traditional propagation methods from seed. Recent studies have indicated that in vitro cloning via somatic embryogenesis is the most promising alternative for the large-scale production of new coconut palms. This paper provides a review on the status and prospects for the application of somatic embryogenesis to mass clonal propagation of coconut.

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Cell suspension culture: A potential in vitro culture method for clonal propagation of coconut plantlets via somatic embryogenesis

Cited by 25 publications

References 81 publications

Effects of Surface Sterilization Protocol and Haustorium Removal on Germination and Growth of Hybrid PB121 Coconut Palm (Cocos nucifera L.) Zygotic Embryos Cultured in vitro

Effects of Surface Sterilization Protocol and Haustorium Removal on Germination and Growth of Hybrid PB121 Coconut Palm (Cocos nucifera L.) Zygotic Embryos Cultured in vitro

Comparative Study of the Genetic and Biochemical Variability of Polyscias filicifolia (Araliaceae) Regenerants Obtained by Indirect and Direct Somatic Embryogenesis as a Source of Triterpenes

Cloning Coconut via Somatic Embryogenesis: A Review of the Current Status and Future Prospects

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