Cell/surface interactions and adhesion on Ti‐6Al‐4V: Effects of surface texture

Abstract

This paper presents the results of an experimental study of the effects of surface texture on the interactions between human osteo-sarcoma (HOS) cells and Ti-6Al-4V. These include the Ti-6Al-4V with polished (smooth); Al(2)O(3) blasted (rough); and laser micro-grooved geometries with controlled spacings and depths. Immuno-fluorescence staining of adhesion proteins (actin and vinculin) was used to study the spreading and adhesion of HOS cells in 2 day culture experiments. Quantitative measures of adhesion were also obtained using an enzymatic detachment assay. The results are discussed within the context of existing theories of cell adhesion. The implications of the results are also examined for the design of textured surfaces in biomedical systems.

Effects of structural property and surface modification of Ti6Ta4Sn scaffolds on the response of SaOS2 cells for bone tissue engineering

“…As the surface layers of the AH and HA treatments were only about 0.2 and 1.0 m thick, the pattern and roughness of both treated surfaces still maintained the characteristics of the substrates, i.e. the grinding groovy patterns with the roughness of ∼0.6 m. The morphology of cells on both treated surfaces was similar claviform shapes, due to the fact that the surface roughness and pattern affect the adhesion and morphology of SaOS 2 cells [1,52]. The cells on both surfaces appeared to align in the direction of the grinding grooves.…”

Laser surface textured titanium alloy (Ti–6Al–4V): Part 1 – Surface characterization

“…Yu et al [13] reported that the average coefficient of friction is minimum when the dimple depth is in the range of 8-10 m [13]. On the other hand, grooves with a dimension of 11 μm width and 10 μm depth offers a positive correlation between cell orientation and cell adhesion [14,15]. The adhesion strength Page 3 of 48 A c c e p t e d M a n u s c r i p t 3 measurement shows that a highest cell retention was on linear textured surface with 20 m spacing [15].…”

“…In the past, studies have been undertaken to understand the effect of texture dimension and orientation on cell attachments on to Ti-6Al-4V surfaces [13][14][15][16]. Yu et al [13] reported that the average coefficient of friction is minimum when the dimple depth is in the range of 8-10 m [13].…”

Structuring of titanium using a nanosecond-pulsed Nd:YVO4 laser at 1064 nm

“…For example, coating of bioactive material (e.g., bioactive tricalcium phosphate) on titanium surface has been utilized in some implants [3]. It is also known that generating microstructures on a titanium surface improves its biocompatibility, and efforts have been made in this direction [e.g., [4][5][6].…”

“…From the laser processing point of view, traditionally, pulsed laser sources operating from UV to IR and with pulse durations from nanosecond to femtosecond have been employed for generation of self-organized microstructures on various metal surfaces [e.g., [5][6][7][8][9][10][11][12]. Recently, we reported on nanosecond-pulsed laser-assisted manufacture of highly organized, reproducible, and periodic microstructure arrays on stainless steel and copper substrates in standard atmospheric environment (room temperature and normal pressure) [13,14].…”