Cell surface immunoglobulin. XI. The appearance of an IgD-like molecule on murine lymphoid cells during ontogeny.

Vitetta, Ellen S.; Melcher, Ulrich; McWilliams, Michael; Lamm, Michael E.; Phillips‐Quagliata, Julia M.; Uhr, Jonathan W.

doi:10.1084/jem.141.1.206

Cited by 182 publications

(53 citation statements)

References 21 publications

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“…1). 5 chains increase from nondetectable levels to approximately 20% of total cell surface immunoglobulin between day 2 and day 12 of age (16,19), while the normal adult ratio of IgD to IgM is as high as 2:1. On the other hand, the density of cell surface IgM per cell does not change significantly during this period (16), although the percent of IgM-bearing cells in the spleen does increase rapidly.…”

Section: Resultsmentioning

confidence: 99%

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Regulation of immunoglobulin D synthesis in murine neonatal B lymphocytes.

Yuan¹

1986

Mol. Cell. Biol.

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We analyzed the regulatory basis for the lower expression of immunoglobulin D (IgD) in lymphocytes from neonatal mice of various ages. The results indicate that the relative transcriptional rate of RNA for 8 chains is similar to adult levels even in cells which have not started to express IgD. These results suggest that very early after the initiation of > gene transcription, a defined fraction of polymerases is programmed to progress through the termination site to the 8 exons regardless of the developmental stage of the cell. Similar results were obtained from adult CBA/N mice whose spleens contain a large fraction of cells expressing low levels of TgD. On the other hand, the relative steady state level of mRNA in neonatal lymphocytes is approximately half of that in adults, suggesting that there may be differences'in the processing or stability of the nascent transcript. In addition, measurements of the in vivo translation rate show that an inefficient 8 polypeptide chain processing machinery in neonatal lymphocytes is also an important factor contributing to the reduced expression of IgD.

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Section: Resultsmentioning

confidence: 99%

“…Immunoglobulin M (IgM) is the first and only isotype to be expressed on immature B lymphocytes, while IgD is gradually acquired during the first weeks of life (16,19,27). In the mouse, surface IgD does not approximate adult levels until 3 weeks after birth (27).…”

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confidence: 99%

Regulation of immunoglobulin D synthesis in murine neonatal B lymphocytes.

Yuan¹

1986

Mol. Cell. Biol.

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“…Only activity against h-chains could be detected by Ouchterlony analysis, and after conjugation to fluorescein, by immunoflurescence staining of fixed cell preparations of various mouse myelomas . Moreover, these antibodies did not precipitate 8-like chains (1,37) from solubilized spleen cells which had been radiolabeled by lactoperoxidasecatalyzed iodination (E . Vitetta, personal communication) .…”

Section: Methodsmentioning

confidence: 99%

Differences in susceptibility of mature and immature mouse B lymphocytes to anti-immunoglobulin-induced immunoglobulin suppression in vitro. Possible implications for B-cell tolerance to self.

Raff¹,

Owen²,

Cooper³

et al. 1975

The Journal of Experimental Medicine

213

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Purified goat antibodies against mouse mu-chains and rabbit antibodies against mouse Ig determinants, and their Fab fragments, inhibited the development of IgM-bearing B cells in explant cultures of 14-day mouse fetal liver, and caused the disappearance of cell surface IgM in explant and dissociated cell cultures of more developed lymphoid tissues. While treatment of cultures of fetal or newborn liver, or adult bone marrow, with low concentrations (less than or equal to 10 mug/ml) of anti-Ig for less than or equal to 24 h caused the complete, but reversible, disappearance (modulation) of cell surface IgM, treatment for greater than or less than 48 h produced irreversible IgM suppression. In contrast, anti-Ig-induced suppression of cell surface IgM in cultures of adult spleen or lymph nodes required much higher concentrations of antibody (greater than or equal to 100 mug/ml) and was always reversible. These differences between immature and mature IgM-bearing cells could not be related to differences in the amount of surface IgM on the cells. The remarkable sensitivity of newly formed B cells to IgM modulation and irreversible IgM suppression when ligands bind to their Ig receptors, may have important implications for B-cell tolerance to self antigens.

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“…Scher et al previously demonstrated that Ig + cells which bear a low-to- (9,16). To examine the ratio of surface IgM to IgD on those B lymphocytes of F~ females which were not susceptible to lysis by the absorbed antiserum, we treated F~ female spleen cells with the absorbed antiserum and C. Live cells were recovered by Ficoll-Hypaque centrifugation, labeled with '25I using the lactoperoxidase technique, solubilized with 0.5% NP-40 in phosphate-buffered saline, and the surface Ig precipitated as noted in the Materials and Methods section.…”

Section: Analysis Of Cellmentioning

confidence: 99%

B-lymphocyte heterogeneity: development and characterization of an alloantiserum which distinguishes B-lymphocyte differentiation alloantigens.

Ahmed¹,

Scher²,

Sharrow³

et al. 1977

The Journal of Experimental Medicine

200

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CBA/N mice and F1 male mice, which are hemizygous for the CBA/N X chromosome, have an immune defect which is associated with the absence (deficiency) of a subpopulation of mature or late developing B lymphocytes. This characteristic was utilized to develop an antiserum that was specific for this subclass of B cells. C57BL/L mice were immunized with DBA/2 spleen calls, and the resulting antisera was absorbed with lymphoid cells derived from immunologically abnormal (CBA/N female X DBA/2 male)F1 male mice. The absorbed antisera was cytotoxic for a subpopulation of lymphocytes that was present in the spleens of adult DBA/2 and (CBA/N female X DBA/2 male)F1 female mice. The cells killed by the absorbed antisera were Ig-bearing, complement receptor-bearing B lymphocytes, which had a low-to-intermediate density of total surface Ig. Moreover, the cells remaining after treatment of adult (CBA/N female X DBA/2 male)F1 female spleen cells with the absorbed antisera and C had a high ratio of surface IgM to IgD. The development of this cytotoxic alloantisera, which is specific for a late developing (mature) subpopulation of B lymphocytes, will allow the functional characterization of subclasses of B lymphocytes.

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Cell surface immunoglobulin. XI. The appearance of an IgD-like molecule on murine lymphoid cells during ontogeny.

Cited by 182 publications

References 21 publications

Regulation of immunoglobulin D synthesis in murine neonatal B lymphocytes.

Regulation of immunoglobulin D synthesis in murine neonatal B lymphocytes.

Differences in susceptibility of mature and immature mouse B lymphocytes to anti-immunoglobulin-induced immunoglobulin suppression in vitro. Possible implications for B-cell tolerance to self.

B-lymphocyte heterogeneity: development and characterization of an alloantiserum which distinguishes B-lymphocyte differentiation alloantigens.

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