Cell sources for regenerative medicine of the liver and endoderm organs: strategies and perspectives

Carpino, Guido; Gaudio, Eugenio

doi:10.21037/sci.2016.11.11

Cited by 2 publications

(1 citation statement)

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“…The use of isolated human liver cells in research and development is being increasingly proposed. The use of freshly isolated hepatocytes may be hampered by weak tolerance to cryopreservation and bacterial contamination leading to consider other cell populations [11]. Adult stem/progenitor cells are undifferentiated cells that may differentiate into functional like-hepatic cells, proliferate and heal the injured tissue [12].…”

Section: Resultsmentioning

confidence: 99%

Immuno-comparative screening of adult-derived human liver stem/progenitor cells for immune-inflammatory-associated molecules

et al. 2021

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Objective One of the main challenges in liver cell therapy is the replacement of damaged cells and the induction of a tolerogenic microenvironment to promote graft acceptance by the recipient. Adult-derived human liver stem/progenitor cells (ADHLSCs) are currently evaluated at the clinical levels as a promising pro-regenerative and immune-modulatory tool. The expression profile of several immunological molecules may influence the local immune-inflammatory response and, therefore, modulate the tissue healing process. To increase the quality and safety of ADHLSCs before transplantation requires an appropriate analysis and characterization of their pattern expression of immune-inflammatory-associated molecules. Methods The expression of 27 molecules belonging to T-cell co-stimulatory pathway, CD47 partners, Ikaros family, CD300 family and TNF family were analyzed using flow cytometry. We compared their expression profiles to PBMCs, hepatocytes and ADHLSCs in both expansion and after hepatogenic differentiation culture conditions. Results This original immuno-comparative screening revealed that liver cell populations do not constitutively present significant immunological pattern compared to PBMCs. Moreover, our findings highlight that neither the expansion nor the hepatogenic differentiation induces the expression of immune-inflammatory molecules. The detailed expression characteristics (percentage of positive cells and median fluorescence intensity) of each molecule were analyzed and presented. Conclusion By analyzing 27 relevant molecules, our immuno-comparative screening demonstrates that ADHLSCs keep a non-immunogenic profile independent of their expansion or hepatogenic differentiation state. Accordingly, the immunological profile of ADHLSCs seems to support their safe and efficient use in liver tissue therapeutic repair strategy.

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Section: Resultsmentioning

confidence: 99%