Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device

Robert, Damien; Pamme, Nicole; Conjeaud, Hélène; Gazeau, Florence; Iles, Alexander; Wilhelm, Claire

doi:10.1039/c0lc00656d

Cited by 133 publications

(84 citation statements)

References 60 publications

(62 reference statements)

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“…Magnetic cell manipulation that applies to magnetophoresis can also be applied to magnetic cell sorting (Figure 13), where we can separate cells in respect to their magnetic load [38]. This may be particularly advantageous when we want a precise and homogenous iron load within a cell fraction and/or want to eliminate poorly loaded cells that would, for example, be less detectable by MRI or less responsible to magnetic targeting.…”

Section: Magnetic Manipulation Of Cells: From Cell Sorting To Magnetimentioning

confidence: 99%

Cell labeling with magnetic nanoparticles: Opportunity for magnetic cell imaging and cell manipulation

et al. 2013

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This tutorial describes a method of controlled cell labeling with citrate-coated ultra small superparamagnetic iron oxide nanoparticles. This method may provide basically all kinds of cells with sufficient magnetization to allow cell detection by high-resolution magnetic resonance imaging (MRI) and to enable potential magnetic manipulation. In order to efficiently exploit labeled cells, quantify the magnetic load and deliver or follow-up magnetic cells, we herein describe the main requirements that should be applied during the labeling procedure. Moreover we present some recommendations for cell detection and quantification by MRI and detail magnetic guiding on some real-case studies in vitro and in vivo.

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Section: Magnetic Manipulation Of Cells: From Cell Sorting To Magnetimentioning

confidence: 99%

Cell labeling with magnetic nanoparticles: Opportunity for magnetic cell imaging and cell manipulation

et al. 2013

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“…Several groups have also described the use of microfluidic devices to deflect particles across streamlines and into different outlets based on their different magnetic contents. 225,226 In these systems, we observed that bacteria motility is also a challenge.…”

Section: Introductionmentioning

confidence: 97%

Acute and Chronic Neural Stimulation via Mechano-Sensitive Ion Channels

Tay

2018

Springer Theses

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Neural stimulation techniques for eliciting calcium influx can elucidate the physiological roles of specific neural populations. To overcome some of the limitations of existing techniques such as poor specificity and noxious effects of heat, I developed a technology for non-invasive control of neural activities using magnetic forces and magnetic nanoparticles (MNPs) which offer deep tissue penetration and controllable dosage. Extensive investigations with different neurotoxins and experimental conditions support that the mechanism of magnetic stimulation that involves membrane-bound MNPs transducing magnetic forces into mechanical stretching of the cell membrane to enhance the opening probability of mechano-sensitive N-type calcium ion channels to induce calcium influx.Making use of the ability of neural networks to actively regulate their ratio of excitatory to inhibitory ion channel/receptor, I also performed chronic magnetic stimulation on fragile X iii syndrome (FXS) model neural networks. We found that chronic magnetic stimulation reduced the density of N-type calcium ion channels whose expression is increased in FXS. This technique demonstrates the potential of using bio-magnetic/mechanical forces to modulate expressions of mechano-sensitive ion channels where they are over-expressed in diseases such as abnormal nociception.Nonetheless, there are still a few areas where the technique can be improved. Firstly, it is the use of MNPs with more uniform properties to have greater control on magnetic stimulations.Secondly, the technique needs to be useful for in vivo studies. Therefore, I started researching on magnetotactic bacteria (MTB) which produce biological MNPs with superior properties such as uniform sizes and highly homogenous magnetic properties with the goal of harvesting MNPs from them.MTB, however, grow extremely slowly and the number of MNPs produced/bacterium is low. One way to overcome this problem is to evolve MTB over-producers of MNPs but this strategy is constrained by the absence of a selection platform that is quantitative and offer high throughput. To overcome this problem, I combined random chemical mutagenesis and selection using a magnetic ratcheting platform to generate and isolate MTB over-producers that produce twice as many MNPs/bacterium after 5 rounds of mutation/selection. I next designed a magnetic microfluidic device and demonstrate as a proof of concept, that it can be coupled to a bioreactor for high throughput microfluidic selection of MTB over-producers.iv

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“…In the past few years, several microfluidic based magnetic sorting concepts have been investigated to capture magnetic beads or magnetically labeled cells. [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] However, these devices are generally hampered by complex fabrication processes and low volumetric throughputs. In many earlier studies, flow rates were limited to less than 1 ml/h, 9,10 which are not practical for many realworld applications.…”

Section: Introductionmentioning

confidence: 99%

On-chip magnetophoretic isolation of CD4 + T cells from blood

Darabi

Guo

2013

Biomicrofluidics

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This paper presents the design, fabrication, and testing of a magnetophoretic bioseparation chip for the rapid isolation and concentration of CD4 þ T cells from the peripheral blood. In a departure from conventional magnetic separation techniques, this microfluidic-based bioseperation device has several unique features, including locally engineered magnetic field gradients and a continuous flow with a buffer switching scheme to improve the performance of the separation process. Additionally, the chip is capable of processing significantly smaller sample volumes than conventional methods and sample losses are eliminated due to decreased handling. Furthermore, the possibility of sample-to-sample contamination is reduced with the disposable format. The overall dimensions of the device were 22 mm by 60 mm by 1 mm, approximately the size of a standard microscope slide. The results indicate a cell purity of greater than 95% at a sample flow rate of 50 ml/h and a cell recovery of 81% at a sample flow rate of 10 ml/h. The cell purity was found to increase with increasing the sample flow rate. However, the cell recovery decreases with an increase in the flow rate. A parametric study was also performed to investigate the effects of channel height, substrate thickness, magnetic bead size, and number of beads per cell on the cell separation performance. V C 2013 AIP Publishing LLC. [http://dx

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Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device

Cited by 133 publications

References 60 publications

Cell labeling with magnetic nanoparticles: Opportunity for magnetic cell imaging and cell manipulation

Cell labeling with magnetic nanoparticles: Opportunity for magnetic cell imaging and cell manipulation

Acute and Chronic Neural Stimulation via Mechano-Sensitive Ion Channels

On-chip magnetophoretic isolation of CD4 + T cells from blood

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