Cell-sized lipid vesicles for cell-cell synaptic therapies

Vallejo, Derek; Lee, S H; Lee, D; Zhang, C; Rapier, Crystal E; Chessler, Steven; Lee, A P

doi:10.1142/s233954781750011x

Cited by 4 publications

(12 citation statements)

References 41 publications

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“…The high stability allows DEDs to be stored for a long time and then be converted into SCMs upon usage. [ 34 ] A detailed description of the channel improvement can be found in the supporting information (Supporting Information S1–S5).…”

Section: Resultsmentioning

confidence: 99%

“…To generate a cell‐like lipid bilayer membrane, the DEDs were converted into SCMs by the dewetting mechanism. As presented by Vallejo et al., [ 34 ] the DEDs can be kept for months and then be transformed into SCMs by controlling the interfacial energies. DEDs have a thin oil shell, which looks like a red ring under a fluorescent microscope (Figure 2g).…”

Section: Resultsmentioning

confidence: 99%

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Cell‐Sized Lipid Vesicles as Artificial Antigen‐Presenting Cells for Antigen‐Specific T Cell Activation

Chen

Agrawal

et al. 2023

Adv Healthcare Materials

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In this study, efficient T cell activation is demonstrated using cell‐sized artificial antigen‐presenting cells (aAPCs) with protein‐conjugated bilayer lipid membranes that mimic biological cell membranes. The highly uniform aAPCs are generated by a facile method based on standard droplet microfluidic devices. These aAPCs are able to activate the T cells in peripheral blood mononuclear cells, showing a 28‐fold increase in interferon gamma (IFNγ) secretion, a 233‐fold increase in antigen‐specific CD8 T cells expansion, and a 16‐fold increase of CD4 T cell expansion. The aAPCs do not require repetitive boosting or additional stimulants and can function at a relatively low aAPC‐to‐T cell ratio (1:17). The research presents strong evidence that the surface fluidity and size of the aAPCs are critical to the effective formation of immune synapses essential for T cell activation. The findings demonstrate that the microfluidic‐generated aAPCs can be instrumental in investigating the physiological conditions and mechanisms for T cell activation. Finally, this method demonstrates the feasibility of customizable aAPCs for a cost‐effective off‐the‐shelf approach to immunotherapy.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Cell‐Sized Lipid Vesicles as Artificial Antigen‐Presenting Cells for Antigen‐Specific T Cell Activation

Chen

Agrawal

et al. 2023

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…The high stability allows DEDs to be stored for a long time and then be converted into SCMs upon usage. [34] A detailed description of the channel improvement can be found in the supporting information (S 1-S 5).…”

Section: Resultsmentioning

confidence: 99%

“…Finally, the aAPC-BLM platform has the potential to be applied to a variety of antigens, such as tetanus (S 17) and treatments, such as inducing insulin secretion in pancreatic β cells. [34] In future studies, the efficacy can potentially be further enhanced by encapsulating some signaling molecules and adjusting the stiffness of aAPC-BLMs.…”

Section: Aapc-blms Expand Significantly Higher Nyeso-specific Cd4 And...mentioning

confidence: 99%

Cell-Sized Lipid Vesicles as Artificial Antigen-Presenting Cells for Antigen-Specific T Cell Activation

Chen

Agrawal

et al. 2022

Preprint

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In this study, efficient T cell activation is demonstrated using cell-sized artificial antigen-presenting cells (aAPCs) with protein-conjugated bilayer lipid membranes that mimic biological cell membranes. The highly uniform aAPCs are generated by a facile method based on standard droplet microfluidic devices. These aAPCs are able to activate the T cells in peripheral blood mononuclear cells (PBMCs), showing a 28-fold increase in IFNg secretion, a 233-fold increase in antigen-specific CD8 T cells expansion, and a 16-fold increase of CD4 T cell expansion. The aAPCs do not require repetitive boosting or additional stimulants and can function at a relatively low aAPC-to-T cell ratio (1-to-17). The research presents strong evidence that the surface fluidity and size of the aAPCs are critical to the effective formation of immune synapses essential for T cell activation. The findings demonstrate that the microfluidic-generated aAPCs can be instrumental in investigating the physiological conditions and mechanisms for T cell activation. Finally, this method demonstrates the feasibility of customizable aAPCs for a cost-effective off-the-shelf approach to immunotherapy.

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“…A double emulsion precursor form has been utilized to improve the storage limitations of all kinds of cell-sized lipid vesicles (CLVs). The therapeutic potential of these lipid vesicles has been demonstrated utilizing them to present transmembrane proteins like neuroligin-2 to pancreatic β-cells, stimulating insulin secretion with the formation of cell–cell synapses [139].…”

Section: Droplet-based Microfluidics For Cellomicsmentioning

confidence: 99%

Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology

2019

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Recently, droplet-based microfluidic systems have been widely used in various biochemical and molecular biological assays. Since this platform technique allows manipulation of large amounts of data and also provides absolute accuracy in comparison to conventional bioanalytical approaches, over the last decade a range of basic biochemical and molecular biological operations have been transferred to drop-based microfluidic formats. In this review, we introduce recent advances and examples of droplet-based microfluidic techniques that have been applied in biochemistry and molecular biology research including genomics, proteomics and cellomics. Their advantages and weaknesses in various applications are also comprehensively discussed here. The purpose of this review is to provide a new point of view and current status in droplet-based microfluidics to biochemists and molecular biologists. We hope that this review will accelerate communications between researchers who are working in droplet-based microfluidics, biochemistry and molecular biology.

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Cell-sized lipid vesicles for cell-cell synaptic therapies

Cited by 4 publications

References 41 publications

Cell‐Sized Lipid Vesicles as Artificial Antigen‐Presenting Cells for Antigen‐Specific T Cell Activation

Cell‐Sized Lipid Vesicles as Artificial Antigen‐Presenting Cells for Antigen‐Specific T Cell Activation

Cell-Sized Lipid Vesicles as Artificial Antigen-Presenting Cells for Antigen-Specific T Cell Activation

Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology

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