Cell Separation

“…The experiment was performed on 96-well plates and read on the Thermoscientific ® Multiskan FC 96-well plate reader (SN 357-914771) at 620 nm. The hFOB 1.19 ATCC line of osteoblasts was used to measure cell viability, osteoblasts are a type of cell without specific requirements for growth and proliferation, although standard conditions for cell culture were performed in this case: 37 °C in an atmosphere of 5% CO2, 95 % air (9) . Measurements at 3, 7, 14, and 21 d were performed to observe how the cells were growing in the plate from an initial seeding of 1,000 cells.…”

Section: Evaluation Of Cell Proliferationmentioning

confidence: 99%

“…FBS is the main culture media supplement since it provides more than 1,000 nutritional components for cells. These include amino acids, proteins, vitamins (particularly fatsoluble vitamins such as A, D, E, and K), carbohydrates, lipids, hormones, growth factors, minerals, and trace elements (9) . In addition, serum buffers inactivate the culture medium proteolytic enzymes, increase the average viscosity, and maintain the conditions for the growth surface of the culture container (10,11) .…”

Section: Introductionmentioning

confidence: 99%

Caracterización del suero bovino fetal proveniente de la industria cárnica mexicana en el cultivo celular

Preciado-Gutiérrez

Masuoka

Barrera-Bernal

et al. 2023

Rev. Mex. Cienc. Pecu.

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The present work aims to characterize a fetal bovine serum (FBS) obtained from the meat industry for use in cell culture. FBS is the most widely used supplement for cell culture since its complex composition provides the necessary nutrients for the growth of most cells. It is a by-product of the meat industry, and its availability and production depend mainly on two uncontrollable external factors, climatic conditions and changes in beef consumption. According to the strict quality features of the International Serum Industry Association (ISIA), tests for total proteins, osmolarity, presence or absence of pathogenic biological agents, pH, DNA concentration, biological contaminants, negative results, and cell viability were performed. The characterization of the serum in the DNA and total protein concentration tests showed significant differences. Additionally, osmolarity and pH did not present significant differences between groups. Regarding the viability test, no complication for cell growth was observed despite the differences found in the characterization. The results showed that the serum obtained from the meat industry could maintain cell cultures and allow cell proliferation compared to commercial serum. Furthermore, if FBS is not available, some kinds of plasma can be used as a surrogate to maintain cell cultures.

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“…The data of optical density were subjected to statistical analysis in order to calculate the concentration of CPA cause a 50 % reduction in cell viability for each cell line (10).…”

Section: Determined Of Cytotoxicitymentioning

confidence: 99%

Cytotoxic and apoptotic effects of cyproterone acetate against cancer cells and normal cells

Al-Saily¹,

Al-Halbosiy²,

Al-Hady³

2019

JoBRC

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Back ground: Cyproterone acetate (CPA) is a steroidal anti-androgen inhibits the testosterone and DHT action it is used as a medicine for prostate cancer by association with the androgen receptors located on the surface of prostate cells, thus preventing the association of testosterone with receptors. Objective: Investigation of the anticancer activities of Cyproterone acetate against cancer cell lines testicular (Tera-1), macrophage (RAW 264.7) in comparison to non- tumorigenic fetal hepatic cell line (WRL-68). Material and method: The cytotoxic effect of CPA was investigated according to selected parameters including: MTT assay as assay of cell function to determined cell viability, high content screening (HCS) technique for the apoptosis of cell. Only the most cytotoxic concentration of CPA and the most sensitive cells as assayed by MTT was selected to complete the other test: (HCS). (MTT) assay was carried out at the Centre of Biotechnology Research’s, Al- NahrainUniversityBaghdad,Iraq . The HCS assay was performed at the Centre for Natural Product Research and Drug Discovery, Department of Pharmacology,Faculty of Medicine, University of Malaya, KualaLumpur, Malaysia Results: The most significant cytotoxic effect of Cyproterone acetate towards 2 cancer cell lines was obtained when its concentration was 1.25mg/mL. The Tera-1 Cells were more sensitive to Cyproterone acetate compared with RAW264.7 and WRL-68. cells. There was a significant decrease in valid cell count, nuclear intensity and mitochondrial membrane potential (MMP) when CPA (400 μg/mL)was used compared with doxorubicin (20 μg/mL) as a standard. Also, there was a significant increase in membrane permeability and cytochrome C releasing when CPA (400μg/ml) was used compared with positive control. Conclusion: CPA showed cytotoxic effects against the Tera-1 and RAW264.7 cancer cell lines while the WRL-68. Cells was not affected as determined in-vitro by the MTT assay. The HCStechnique also showed toxic effect towards Tera-1.

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Cell Separation

Cited by 9 publications

References 34 publications

In vitro regulation of cell behavior by calcium phosphates synthesized by the mechanochemical method

In vitro regulation of cell behavior by calcium phosphates synthesized by the mechanochemical method

Caracterización del suero bovino fetal proveniente de la industria cárnica mexicana en el cultivo celular

Cytotoxic and apoptotic effects of cyproterone acetate against cancer cells and normal cells

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