Cell-Penetrating Antimicrobial Peptides Derived from an Atypical Staphylococcal δ-Toxin

Deeyagahage, Kathyana; Ruzzini, Antonio C.

doi:10.1128/spectrum.01584-21

Cited by 4 publications

(1 citation statement)

References 39 publications

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“…Further inspection of these genomic regions and gene clusters through Artemis and BLASTp also identified genes encoding putative δ-lysins and phenol soluble modulins (PSMs) within each of the 11 sequenced genomes. δ-lysins and PSMs are small, Staphylococcus-associated bioactive peptides [11,22,23] that have previously been identified in human-derived CoNS and observed to exert antimicrobial activity against some gram-positive pathogens [11,24]. A summary of all AOIs identified in the preliminary investigation of the sequenced genomes using BAGEL4 is presented in Table 2.…”

Section: Analysis Of Whole Genome Sequencing Of Shortlisted Cons Isol...mentioning

confidence: 99%

Inhibition of Clinical MRSA Isolates by Coagulase Negative Staphylococci of Human Origin

Twomey,

O’Connor,

Coffey

et al. 2024

Antibiotics

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Staphylococcus aureus is frequently highlighted as a priority for novel drug research due to its pathogenicity and ability to develop antibiotic resistance. Coagulase-negative staphylococci (CoNS) are resident flora of the skin and nares. Previous studies have confirmed their ability to kill and prevent colonization by S. aureus through the production of bioactive substances. This study screened a bank of 37 CoNS for their ability to inhibit the growth of methicillin-resistant S. aureus (MRSA). Deferred antagonism assays, growth curves, and antibiofilm testing performed with the cell-free supernatant derived from overnight CoNS cultures indicated antimicrobial and antibiofilm effects against MRSA indicators. Whole genome sequencing and BAGEL4 analysis of 11 CoNS isolates shortlisted for the inhibitory effects they displayed against MRSA led to the identification of two strains possessing complete putative bacteriocin operons. The operons were predicted to encode a nukacin variant and a novel epilancin variant. From this point, strains Staphylococcus hominis C14 and Staphylococcus epidermidis C33 became the focus of the investigation. Through HPLC, a peptide identical to previously characterized nukacin KQU-131 and a novel epilancin variant were isolated from cultures of C14 and C33, respectively. Mass spectrometry confirmed the presence of each peptide in the active fractions. Spot-on-lawn assays demonstrated both bacteriocins could inhibit the growth of an MRSA indicator. The identification of natural products with clinically relevant activity is important in today’s climate of escalating antimicrobial resistance and a depleting antibiotic pipeline. These findings also highlight the prospective role CoNS may play as a source of bioactive substances with activity against critical pathogens.

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Section: Analysis Of Whole Genome Sequencing Of Shortlisted Cons Isol...mentioning

confidence: 99%

Inhibition of Clinical MRSA Isolates by Coagulase Negative Staphylococci of Human Origin

Twomey,

O’Connor,

Coffey

et al. 2024

Antibiotics

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Cell Penetrating Peptides: Classification, Mechanisms, Methods of Study, and Applications

et al. 2023

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Cell‐penetrating peptides (CPPs) encompass a class of peptides that possess the remarkable ability to cross cell membranes and deliver various types of cargoes, including drugs, nucleic acids, and proteins, into cells. For this reason, CPPs are largely investigated in drug delivery applications in the context of many diseases, such as cancer, diabetes, and genetic disorders. While sharing this functionality and some common structural features, such as a high content of positively charged amino acids, CPPs represent an extremely diverse group of elements, which can differentiate under many aspects. In this review, we summarize the most common characteristics of CPPs, introduce their main distinctive features, mechanistic aspects that drive their function, and outline the most widely used techniques for their structural and functional studies. We highlight current gaps and future perspectives in this field, which have the potential to significantly impact the future field of drug delivery and therapeutics.

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Classes and Applications of Cell-Penetrating Peptides

Langel

2023

CPP, Cell-Penetrating Peptides

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Cell-Penetrating Antimicrobial Peptides Derived from an Atypical Staphylococcal δ-Toxin

Cited by 4 publications

References 39 publications

Inhibition of Clinical MRSA Isolates by Coagulase Negative Staphylococci of Human Origin

Inhibition of Clinical MRSA Isolates by Coagulase Negative Staphylococci of Human Origin

Cell Penetrating Peptides: Classification, Mechanisms, Methods of Study, and Applications

Classes and Applications of Cell-Penetrating Peptides

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