Classes and Applications of Cell-Penetrating Peptides

Endosomolytic Peptides Enable the Cellular Delivery of Peptide Nucleic Acids

“…Here, we assess the efficiency of PNA delivery into human cells as mediated by three peptides: L17E, 27 L17ER 4 (which contains four arginine residues), 32 and R10 (which is a well-known cell-penetrating peptide). 33 To assess delivery, we employ a rigorous quantitative assay based on the corrective splicing of an mRNA that is translated into the green fluorescent protein (GFP) (Scheme 1). First, we compare the cellular uptake of a PNA upon co-treatment with each peptide.…”