2011
DOI: 10.1016/j.actbio.2010.09.040
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Cell patterning using molecular vapor deposition of self-assembled monolayers and lift-off technique

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Cited by 27 publications
(14 citation statements)
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“…In an effort to recapitulate alignment in vitro , a number of nano- and microscale technologies have been developed, including topographical patterning 8 9 10 11 , surface chemical modulation 12 , and mechanical deformation 13 14 of culture substrates, but generally for two-dimensional (2D) settings 15 . For three-dimensional (3D) constructs, several strategies have been introduced to confer anisotropy, which in many cases involve application of strong external forces, such as electric 16 17 or magnetic field 18 19 and mechanical stress 19 20 21 22 23 .…”
mentioning
confidence: 99%
“…In an effort to recapitulate alignment in vitro , a number of nano- and microscale technologies have been developed, including topographical patterning 8 9 10 11 , surface chemical modulation 12 , and mechanical deformation 13 14 of culture substrates, but generally for two-dimensional (2D) settings 15 . For three-dimensional (3D) constructs, several strategies have been introduced to confer anisotropy, which in many cases involve application of strong external forces, such as electric 16 17 or magnetic field 18 19 and mechanical stress 19 20 21 22 23 .…”
mentioning
confidence: 99%
“…The molecular vapor deposition (MVD) technique (MVD™ is a trademark of Applied MicroStructures, Inc.) was originally developed for the fabrication of micro-electromechanical systems (MEMS) [79]. This methodology comprises injectors that disperse the substance being dispensed into molecular vapor.…”
Section: Methodologies Used To Assemble Ht Screening Platformsmentioning
confidence: 99%
“…The MVD technique has recently been used in the field of biomedical engineering. Jing et al [79, 81] have demonstrated that this new technology is useful for cell assembly and patterning, and the immortalized mouse hypothalamic (GT1–7) neurons and mouse (MC3T3) osteoblast cells have been successfully patterned and maintained for at least 14 days in vitro. Furthermore, this technology is characterized by attractive features including: (i) single-cell resolution, (ii) easy alignment of the patterns with the pre-existing patterns on the substrate, (iii) easy formation of nanoscale patterns (depending on the exposure equipment), (iv) long shelf life of the substrate pattern prior to cell culturing, (v) compatibility with conventional, inverted, optical microscopes for simple visualization of patterns formed on a glass wafer, and (vi) the ability to support patterned cell (osteoblast) networks for at least 2 weeks.…”
Section: Methodologies Used To Assemble Ht Screening Platformsmentioning
confidence: 99%
“…[4][5][6][7][8][9][10][11] Superhydrophobicity with a water contact angle (CA) above 1501 and superhydrophilicity with a CA below 51 are the two extreme cases of wettability and have attracted great interest due to their importance in fundamental research and practical application. 12,13 The differences of wettability can be used to engineer micro-templates with various sized micro-units of domains, which as a unique prospective technique has been widely applied in cell growth, 14,15 fluid microchips, 16 micro-reactors, 17,18 and sensor arrays. 19 Among various applications, the micro-patterning of living cells is attracting more and more attention because it can be used in a broad range of research such as bioassays, 20,21 drugs, 22 tissue engineering, 23 and many fundamental studies of interactions between cells and materials.…”
Section: Introductionmentioning
confidence: 99%