Cell migration in<i>Drosophila</i>optic lobe neurons is controlled by<i>eyeless/Pax6</i>

Morante, Javier; Erclik, Ted; Desplan, Claude

doi:10.1242/dev.056069

Cited by 49 publications

(40 citation statements)

References 34 publications

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“…Therefore, NBs of different ages can be observed together in one snapshot (Egger, Boone, Stevens, Brand, & Doe, 2007; Yasugi, Umetsu, Murakami, Sato, & Tabata, 2008). These NBs generate GMCs, which then produce medulla neurons that form chains below each NB, with the first-born neurons located in the deepest position and latest-born neurons in the most superficial position, near the NB (Hasegawa et al, 2011; Morante, Erclik, & Desplan, 2011). Recently, the developing medulla has emerged as a new powerful system for studying temporal patterning of NBs (Li et al, 2013; Suzuki, Kaido, Takayama, & Sato, 2013).…”

Section: Introductionmentioning

confidence: 99%

Temporal Patterning of Neural Progenitors in Drosophila

Chen

Desplan

2013

Current Topics in Developmental Biology

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Drosophila has recently become a powerful model system to understand the mechanisms of temporal patterning of neural progenitors called neuroblasts (NBs). Two different temporal sequences of transcription factors (TFs) have been found to be sequentially expressed in NBs of two different systems: the Hunchback, Krüppel, Pdm1/Pdm2, Castor, and Grainyhead sequence in the Drosophila ventral nerve cord; and the Homothorax, Klumpfuss, Eyeless, Sloppy-paired, Dichaete, and Tailless sequence that patterns medulla NBs. In addition, the intermediate neural progenitors of type II NB lineages are patterned by a different sequence: Dichaete, Grainyhead, and Eyeless. These three examples suggest that temporal patterning of neural precursors by sequences of TFs is a common theme to generate neural diversity. Cross-regulations, including negative feedback regulation and positive feedforward regulation among the temporal factors, can facilitate the progression of the sequence. However, there are many remaining questions to understand the mechanism of temporal transitions. The temporal sequence progression is intimately linked to the progressive restriction of NB competence, and eventually determines the end of neurogenesis. Temporal identity has to be integrated with spatial identity information, as well as with the Notch-dependent binary fate choices, in order to generate specific neuron fates.

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Section: Introductionmentioning

confidence: 99%

Temporal Patterning of Neural Progenitors in Drosophila

Chen

Desplan

2013

Current Topics in Developmental Biology

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“…Our recent work on the migration has shown that GMC-1àRP2/sib undergo a complex 3-step migration and Wingless (Wg) signaling is necessary for the step 2 and step 3 migrations (Bhat, 2007). Recent work has shown that there is an active migration process going on in the optic lobe (Morante et al, 2011; Hasegawa et al, 2011). For example, medulla cortex cells in the optic lobe oerform two patterns of cell migrations to acquire their final position.…”

Section: Introductionmentioning

confidence: 99%

The Hem protein mediates neuronal migration by inhibiting WAVE degradation and functions opposite of Abelson tyrosine kinase

Zhu

Bhat²

2011

Developmental Biology

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In the nervous system, neurons form in different regions, then they migrate and occupy specific positions. We have previously shown that RP2/sib, a well-studied neuronal pair in the Drosophila ventral nerve cord (VNC), has a complex migration route. Here, we show that the Hem protein, via the WAVE complex, regulates migration of GMC-1 and its progeny RP2 neuron. In Hem or WAVE mutants, RP2 neuron either abnormally migrates, crossing the midline from one hemisegment to the contralateral hemisegment, or does not migrate at al and fail to send out its axon projection. We report that Hem regulates neuronal migration through stabilizing WAVE. Since Hem and WAVE normally form a complex, our data argues that in the absence of Hem, WAVE, which is presumably no longer in a complex, becomes susceptible to degradation. We also find that Abelson Tyrosine kinase affects RP2 migration in a similar manner as Hem and WAVE, and appears to operate via WAVE. However, while Abl negatively regulates the levels of WAVE, it regulates migration via regulating the activity of WAVE. Our results also show that during the degradation of WAVE, Hem function is opposite to that of and downstream of Abl.

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“…melanogaster , the compound eyes and most head structures (head capsule, antenna, ocelli) develop entirely from the eye-antennal discs [75], in which process ey gene is involved in cell proliferation, differentiation and migration/adhesion [76, 77]. The headless phenotype characterized by the lack of structures derived from eye-antennal discs suggests massive cell death during development [56, 78].…”

Section: Discussionmentioning

confidence: 99%

“…As evident from acridine orange staining of RpPax6 expressed larva and rescue of headless by the expression of the baculovirus P35 protein in eye-antennal discs (Fig 8D), the headless phenotype of UAS-RpPax6/ey-GAL4 pharates results from the induction of apoptosis by expression of RpPax6 . Paradoxically, authentic Pax genes are associated with differentiation, proliferation and anti-apoptosis during development process [76, 77]. Inhibition of ey [58, 79] as well as other Pax genes including Pax2 , Pax8 [80], Pax3 and Pax7 [81] induce apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Functional Analysis of Pax6 from the Hydrothermal Vent Tubeworm Ridgeia piscesae

Yuan

Wang

et al. 2016

PLoS ONE

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The paired box 6 (Pax6) gene encodes a transcription factor essential for eye development in a wide range of animal lineages. Here we describe the cloning and characterization of Pax6 gene from the blind hydrothermal vent tubeworm Ridgeia piscesae (RpPax6). The deduced RpPax6 protein shares extensive sequence identity with Pax6 proteins from other species and contains both the paired domain and a complete homeodomain. Phylogenetic analysis indicates that it clusters with the corresponding sequence from the closely related species Platynereis dumerilii (P. dumerilii) of Annelida. Luciferase reporter assay indicate that RpPax6 protein suppresses the transcription of sine oculis (so) in D. melanogaster, interfering with the C-terminal of RpPax6. Taking advantage of Drosophila model, we show that RpPax6 expression is not able to rescue small eye phenotype of ey2 mutant, only to cause a more severe headless phenotype. In addition, RpPax6 expression induced apoptosis and inhibition of apoptosis can partially rescue RpPax6-induced headless phenotype. We provide evidence RpPax6 plays at least two roles: it blocks the expression of later-acting transcription factors in the eye development cascade, and it promotes cell apoptosis. Our results indicate alternation of the Pax6 function may be one of the possible causes that lead the eye absence in vestimentiferan tubeworms.

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Cell migration inDrosophilaoptic lobe neurons is controlled byeyeless/Pax6

Cited by 49 publications

References 34 publications

Temporal Patterning of Neural Progenitors in Drosophila

Temporal Patterning of Neural Progenitors in Drosophila

The Hem protein mediates neuronal migration by inhibiting WAVE degradation and functions opposite of Abelson tyrosine kinase

Cloning and Functional Analysis of Pax6 from the Hydrothermal Vent Tubeworm Ridgeia piscesae

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