Cell microarrays on photochemically modified polytetrafluoroethylene

Mikulíková, Regina; Moritz, Sieglinde; Gumpenberger, Thomas; Olbrich, Michael; Romanin, Christoph; Bačáková, Lucie; Švorčı́k, Václav; Heitz, J.

doi:10.1016/j.biomaterials.2005.02.010

Cited by 63 publications

(47 citation statements)

References 31 publications

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“…One of the most efficient ways to increase the surface area of composites consists on the production of microfibers by electrospinning [22], in which the modification of the processing parameters lead to variations in electrospun fiber morphology, orientation and diameter [23]. In this way, electrospinning can produce fibers and membranes that are able to mimic extracellular matrix (ECM) of biological tissues [24], being increasingly important in the development of scaffolds for a variety of biomedical applications [25,26] by allowing tailoring cell functions, adhesion and proliferation [27] through modulation of surface electrostatic charge [28], wettability [29], mechanical properties [30] and topography. The introduction of fillers in fibers can also contribute to modify bioactivity and even biocompatibility suppression can occur.…”

Section: Introductionmentioning

confidence: 99%

Effect of filler content on morphology and physical–chemical characteristics of poly(vinylidene fluoride)/NaY zeolite-filled membranes

et al. 2014

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Poly(vinylidene fluoride) electrospun membranes have been prepared with different NaY zeolite contents up to 32%wt. Inclusion of zeolites induces an increase of average fiber size from ~200 nm in the pure polymer up to ~500 nm in the composite with 16%wt zeolite content. For higher filler contents, a wider distribution of fibers occurs leading to a broader size distributions between the previous fiber size values.Hydrophobicity of the membranes increases from ~115º water contact angle to ~128º with the addition of the filler and is independent on filler content, indicating a wrapping of the zeolite by the polymer. The water contact angle further increases with fiber alignment up to ~137º. Electrospun membranes are formed with ~80 % of the polymer crystalline phase in the electroactive  phase, independently on the electrospinning processing conditions or filler content. Viability of MC3T3-E1 cells on the composite membranes after 72 h of cell culture indicates the suitability of the membranes for tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Effect of filler content on morphology and physical–chemical characteristics of poly(vinylidene fluoride)/NaY zeolite-filled membranes

et al. 2014

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“…HEK 293 cells are frequently used as model cells for the expression of proteins, especially of membrane bound G-protein coupled receptors (Grantcharova-Angelova 2005). This huge receptor family is of tremendous pharmaceutical relevance, and tools, such as microarrays, enabling the rapid screening of a large number of ligands and receptors are therefore highly demanded (Robbins and Horlick 1998;Mikulikova et al 2005). We apply soft lithography in combination with plasma oxidation and polyelectrolyte multilayer deposition to create patterns of untreated PDMS, oxidized PDMS (ox-PDMS), PAH (poly(allylamine hydrochloride)) and PSS (poly(sodium 4-styrenesulfonate)) in all dual combinations and examine the selectivity of cell attachment during culture over several days.…”

Section: Introductionmentioning

confidence: 99%

Micropatterned surfaces of PDMS as growth templates for HEK 293 cells

Johann

Baiotto²,

Renaud

2007

Biomed Microdevices

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In this paper the easy and reliable preparation of precise micropatterns on PDMS surfaces is described and the growth of HEK 293 cells on those patterns during culture over several days is examined. The first patterning approach described is based on soft-lithography and polyelectrolyte multilayer deposition. Two different soft-lithographic techniques are employed for creating surface patterns of PAH, PSS, untreated and oxidized PDMS. The growth behavior of HEK 293 cells is investigated on all the dual combinations of the four surfaces, and decreasing preference of the cells for the surfaces in the order PAH (-NH2)>ox-PDMS (-OH)>>PSS (-SO3-)>PDMS (-CH3) is revealed. As the second patterning approach a method is introduced, which allows the deposition of gel droplets in a microarray format utilizing differences in the surface wettability. This concept is new and expected to be very useful for various applications. Finally, a speculative explanation for the different cell spreading behavior is provided considering the interplay between individual cell-surface interactions and a permanent cell tractional force.

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“…Appropriate modifications can be achieved by chemical [37], plasma [38], or radiation treatment. For the photo− −induced modification of polymers, excimer lamps or ex− cimer lasers are mainly used [39][40][41].…”

Section: Surface Treatmentmentioning

confidence: 99%

Laser-plasma extreme ultraviolet and soft X-ray sources based on a double stream gas puff target: interaction of the radiation pulses with matter

Bartnik

2015

Opto-Electronics Review

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In this work a review of investigations concerning interaction of intense extreme ultraviolet (EUV) and soft X-ray (SXR) pulses with matter is presented. The investigations were performed using laser-produced plasma (LPP) EUV/SXR sources based on a double stream gas puff target. The sources are equipped with dedicated collectors allowing for efficient focusing of the EUV/SXR radiation pulses. Intense radiation in a wide spectral range, as well as a quasi-monochromatic radiation can be produced. In the paper different kinds of LPP EUV/SXR sources developed in the Institute of Optoelectronics, Military University of Technology are described.Radiation intensities delivered by the sources are sufficient for different kinds of interaction experiments including EUV/SXR induced ablation, surface treatment, EUV fluorescence or photoionized plasma creation. A brief review of the main results concerning this kind of experiments performed by author of the paper are presented. However, since the LPP sources cannot compete with large scale X-ray sources like synchrotrons, free electron lasers or high energy density plasma sources, it was indicated that some investigations not requiring extreme irradiation parameters can be performed using the small scale installations. Some results, especially concerning low temperature photoionized plasmas are very unique and could be hardly obtained using the large facilities.

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Cell microarrays on photochemically modified polytetrafluoroethylene

Cited by 63 publications

References 31 publications

Effect of filler content on morphology and physical–chemical characteristics of poly(vinylidene fluoride)/NaY zeolite-filled membranes

Effect of filler content on morphology and physical–chemical characteristics of poly(vinylidene fluoride)/NaY zeolite-filled membranes

Micropatterned surfaces of PDMS as growth templates for HEK 293 cells

Laser-plasma extreme ultraviolet and soft X-ray sources based on a double stream gas puff target: interaction of the radiation pulses with matter

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