Cell Membrane Transport Mechanisms: Ion Channels and Electrical Properties of Cell Membranes

Kulbacka, Julita; Choromańska, Anna; Rossowska, Joanna; Weżgowiec, Joanna; Saczko, Jolanta; Rols, Marie‐Pierre

doi:10.1007/978-3-319-56895-9_3

Cited by 43 publications

(32 citation statements)

References 91 publications

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“…Membrane Transport module is closely related to cell life, and cell life depends to a large extent on the ability of the membrane to accurately control the solute exchange between the internal and external (environmental) regions. The decrease of membrane transport functional modules may lead to premature apoptosis (Kulbacka et al, 2017). At the same time, membrane transport involves the complex mechanism of passive and active transport of ions and small and medium-sized molecules, and its disorder may affect the metabolism of calcium salts, thus affecting bone metabolism (Pavlos and Friedman, 2017).…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiome Reveals Specific Dysbiosis in Primary Osteoporosis

Xie

Sun

et al. 2020

Front. Cell. Infect. Microbiol.

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Object: Primary osteoporosis (PO) is the most common bone disease, which is characterized by decreased bone mass, damage of bone tissue microstructure, increased bone fragility, and is prone to fracture. Gut microbiome may be involved in bone metabolism of PO through gut-brain axis regulation of immune system and endocrine system, however, the specific mechanism is still unclear. The purpose of this study was to characterize the gut microbiome of patients with PO and its possible role in the occurrence and development of the disease. Methods: Fecal samples were collected from 48 PO patients and 48 healthy controls (HC). The composition of gut microbiome community was analyzed by 16s rDNA amplification sequencing, and the difference of gut microbiome composition between PO patients and HC individuals was compared. PICRUSt was also used to predict the biological function of gut microbiome in patients with PO, and to explore its possible role in the occurrence and development of this disease. The classification model is constructed by random forest algorithm so as to screen the key biomarkers. Result: The diversity of gut microorganisms in PO patients was significantly higher than that in HC group (p < 0.05) and there was significant difference in microbial composition in PO group. The abundance of Dialister (0.036 vs. 0.004, p < 0.001) and Faecalibacterium (0.331 vs. 0.132, p < 0.001) were significantly enriched which were the key flora related to PO. Although no significant correlation between bone mineral density and the richness of microbial communities are found, PICRUST results show that there are a wide range of potential pathways between gut microbiome and PO patients, including genetic information processing, metabolism, environmental information processing, cellular processes, human diseases, and organic systems. Notably, the discriminant model based on dominant microflora can effectively distinguish PO from HC (AUC = 93.56). Conclusions: The findings show that PO is related to the change of gut microbiome, especially the enriched Dialister and Faecalibacterium genera, which give new clues to understand the disease and provide markers for the diagnosis and new strategies for intervention treatment of the disease.

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Section: Discussionmentioning

confidence: 99%

Gut Microbiome Reveals Specific Dysbiosis in Primary Osteoporosis

Xie

Sun

et al. 2020

Front. Cell. Infect. Microbiol.

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“…All aspects of metabolism, including hormone secretion, vitamin synthesis, food digestion and the absorption of nutrients are regulated via cation transmembrane channels (38). These channels are located in a variety of organs and have the ability to function at metabolically appropriate times (39). A number of previous studies have reported on the expression and regulation of various cation channel proteins; however, there are no similar reports for such proteins in canine organs (2,31,(39)(40)(41).…”

Section: Discussionmentioning

confidence: 99%

“…These channels are located in a variety of organs and have the ability to function at metabolically appropriate times (39). A number of previous studies have reported on the expression and regulation of various cation channel proteins; however, there are no similar reports for such proteins in canine organs (2,31,(39)(40)(41). Thus, in the present study, the organ-specific expression and localization of calcium (NCKX3, TRPV2), copper (CTR1, ATP7A), iron (IREG1) and ferroxidase for iron transporting (HEPH) proteins and mRNAs were investigated in the canine duodenum (upper part of the duodenum), kidney (borderline of the cortex and medulla), spleen (borderline of the white and red pulp) and liver (quadrate lobe of the liver).…”

Section: Discussionmentioning

confidence: 99%

Organ‑specific expression of the divalent ion channel proteins NCKX3, TRPV2, CTR1, ATP7A, IREG1 and HEPH in various canine organs

2018

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Transmembrane cation channels include those for calcium, copper and iron ion transport. Each channel has physiological significance, and all have been associated with disease. However, the comparative study of transcriptional‑translational levels in canine organs has not been previously reported. In the present study, organ‑specific expression of calcium channels, including sodium/potassium/calcium exchanger 3 (NCKX3) and transient receptor potential cation channel subfamily V member 2 (TRPV2), copper channels, including high affinity copper uptake protein 1 (CTR1) and copper‑transporting ATPase 1 (ATP7A), and iron channels, including iron‑regulated transporter 1 (IREG1) and hephaestin (HEPH) proteins and their mRNAs were examined in the canine duodenum, kidney, spleen and liver. NCKX3 protein expression was highest in the kidney, moderate in the duodenum, and lowest in the spleen and liver, whereas TRPV2 protein was highly expressed in the kidney, duodenum and liver, and was low in the spleen. The CTR1 protein expression level was highest in the liver, followed (in descending order) by the duodenum, kidney and spleen. The ATP7A protein expression level was highest in the duodenum and lowest in the spleen. The IREG1 protein expression level was highest in the liver, followed (in descending order) by the kidney, duodenum and spleen. The HEPH protein level was high in liver, moderate in the duodenum and kidney, and low in the spleen. The results of the immunohistochemistry analysis demonstrated ion channel protein localizations. These results suggested that cation channel proteins are differentially expressed among canine organs, and they may be involved in organ‑specific functions associated with the maintenance of physiological homeostasis.

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“…across the cell membrane. Carriers physically bind to a specific solute and change their own conformation to release the solute on the other side of the cell membrane; channels form a pore, which can open to allow a specific molecule to pass by diffusion or osmosis (Kulbacka et al, 2017).…”

Section: Membrane Proteins As Potential Osmosensors In the Ivdmentioning

confidence: 99%

Osmosensing, osmosignalling and inflammation: how intervertebral disc cells respond to altered osmolarity

Sadowska¹,

Kameda²,

Krupková³

et al. 2018

eCM

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Intervertebral disc (IVD) cells are naturally exposed to high osmolarity and complex mechanical loading, which drive microenvironmental osmotic changes. Age-and degeneration-induced degradation of the IVD's extracellular matrix causes osmotic imbalance, which, together with an altered function of cellular receptors and signalling pathways, instigates local osmotic stress. Cellular responses to osmotic stress include osmoadaptation and activation of pro-inflammatory pathways. This review summarises the current knowledge on how IVD cells sense local osmotic changes and translate these signals into physiological or pathophysiological responses, with a focus on inflammation. Furthermore, it discusses the expression and function of putative membrane osmosensors (e.g. solute carrier transporters, transient receptor potential channels, aquaporins and acid-sensing ion channels) and osmosignalling mediators [e.g. tonicity responseelement-binding protein/nuclear factor of activated T-cells 5 (TonEBP/NFAT5), nuclear factor kappa-lightchain-enhancer of activated B cells (NF-κB)] in healthy and degenerated IVDs. Finally, an overview of the potential therapeutic targets for modifying osmosensing and osmosignalling in degenerated IVDs is provided.

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Cell Membrane Transport Mechanisms: Ion Channels and Electrical Properties of Cell Membranes

Cited by 43 publications

References 91 publications

Gut Microbiome Reveals Specific Dysbiosis in Primary Osteoporosis

Gut Microbiome Reveals Specific Dysbiosis in Primary Osteoporosis

Organ‑specific expression of the divalent ion channel proteins NCKX3, TRPV2, CTR1, ATP7A, IREG1 and HEPH in various canine organs

Osmosensing, osmosignalling and inflammation: how intervertebral disc cells respond to altered osmolarity

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