Cell membrane coated smart two-dimensional supraparticle for in vivo homotypic cancer targeting and enhanced combinational theranostics

Abstract

Development of intelligent and multifunctional nanoparticle for the diagnosis and treatment of cancer has drawn great attention recently. In this work, we design a smart two-dimensional (2D) supraparticle for tumor targeted magnetic resonance imaging (MRI)/photothermal imaging (PTI) and chemo/photothermal therapy (PTT). Methods: The nanoparticle consists of a manganese dioxide (MnO 2 ) nanosheet coated gold nanorod (GNR) core (loading with chemotherapeutics doxorubicin (DOX)), and cancer cell membrane shell (denoted as CM-DOX-GMNPs). Decoration of cell membrane endows the nanoparticle with greatly improved colloidal stability and homotypic cancer cell targeting ability. Once the nanoparticles enter tumor cells, MnO 2 nanosheets can be etched to Mn 2+ by glutathione (GSH) and acidic hydrogen peroxide (H 2 O 2 ) in the cytosol, leading to the release of DOX. Meanwhile, stimuli dependent releasing of Mn 2+ can act as MRI contrast agent for tumor diagnosis. Illumination with near-infrared (NIR) light, photothermal conversion effect of GNRs can be activated for synergistic cancer therapy. Results: In vivo results illustrate that the CM-DOX-GMNPs display tumor specific MRI/PTI ability and excellent inhibition effect on tumor growth. Conclusion: This bioinspired nanoparticle presents an effective and intelligent approach for tumor imaging and therapy, affording valuable guidance for the rational design of robust theranostics nanoplatform.

Nanozyme-Triggered Cascade Reactions from Cup-Shaped Nanomotors Promote Active Cellular Targeting

“…However, under the same conditions, the temperature of phosphate buffer saline (PBS) solution and deionized water only ascended to 42.0 °C and 36.5 °C, respectively. The photothermal conversion efficiencies of GNCs-Pt-ICG/Tf and GNCs-ICG/Tf are calculated to be 44.31% and 41.09%, respectively, which are comparable to that of commonly used nanomaterials for PTT such as gold nanorods (39.2%)[50], Cu 3 BiS 3 nanorods (40.7%)[51], and Pt-CuS nanoparticles (34.5%) (Figures6(f) and S20)[52]. These results indicate that the deposition of PtNPs has negligible influence on the photothermal performance of GNCs.…”

In vivo co-registered hybrid-contrast imaging by successive photoacoustic tomography and magnetic resonance imaging

“…To harness the high speed imaging capability provided by PAT imaging, we applied intravenous (IV) injection of 200 μl of ICG (0.05 μg/μl) on a day-21 4T1 tumorous mouse after MRI imaging, and then performed PAT temporal imaging of the mouse at 5-minute intervals for 40 mins. ICG, which is a FDAapproved NIR fluorochrome commonly used as a contrast agent in retinal and tumor imaging, is able to metabolize in blood-rich organs and excreted into the bile within one hour [36][37][38] . The registration results and the distribution of ICG identified from the multispectral PAT images are shown in Supplementary Fig.…”

Immune-regulating camouflaged nanoplatforms: A promising strategy to improve cancer nano-immunotherapy

“…In addition, cancer cell membrane-coated nanoparticles reportedly demonstrate homotypic aggregation into the tumor site, depending on the specific interactions of identical surface adhesion molecules as the source of cancer cells, such as epithelial cell adhesion molecule (EpCAM), galectin-3, and N-cadherin [ 27 , 76 ]. A nanoplatform based on the 2D MnO 2 nanosheets wrapped over gold nanorods (loading with DOX), and subsequent cancer cell membranes, was recently developed [ 77 ]. The prepared nanoplatform was endowed with homotypic tumor targeting and immune clearance evading abilities from cancer cell membranes.…”

“… Homotypic cancer-targeting/immune escape MRI/PTI-guided PTT and chemotherapy The nanoplatforms with tumor targeting and immune escape abilities were disrupted to release the loads that afforded a robust result of the imaging-guided PTT/chemotherapy. [ 77 ] The biomimetic vehicle was established by integrating MSC membranes with DOX-loaded mesoporous silica NPs. Escaping immunosurveillance/active tumor targeting Chemotherapy With the acquired immunosurveillance escape and self-position abilities, the MSC membrane-coated NPs showed enhanced therapeutic outcomes with alleviated side effects.…”