Cell membrane-coated biomimetic magnetic nanoparticles for the bio-specific extraction of components from Gualou Guizhi decoction exhibiting activities against oxygen-glucose deprivation/reperfusion injury

Screening of the active Ingredients in Huanglian Jiedu decoction through amide bond-Immobilized magnetic nanoparticle-assisted cell membrane chromatography

“…The cells were then cultured in a cell incubator at 37 °C. The CMs were prepared according to previously reported methods (He et al, 2022a;He et al, 2022b). The amount of CMs was examined using the BCA protein assay.…”

“…Subsequently, 5 mL citrate-sodium citrate buffer (pH 4.0) was added, and the resultant solution was incubated for 30 min to dissociate the specific binding components from the CM. The dissociated solution was collected and fractionated by SPE, according to a previously reported method (He et al, 2022a;He et al, 2022b). This sample was used for mass spectrometry (MS) analysis.…”

“…Moreover, the high accumulation of reactive oxygen species is closely related to glutamate-induced neuronal damage (Pan et al, 2020;Tonsomboon et al, 2021). The Huanglian Jiedu decoction (HLJDD), which originates from "Arcane Essentials from the Imperial Library", is composed of Coptis chinensis Franch (Ranunculaceae; Coptidis Rhizoma), Scutellaria baicalensis Georgi (Lamiaceae; Scutellariae Radix), Phellodendron amurense Rupr (Rutaceae; Phellodendri Amurensis cortex), and Gardenia jasminoides Ellis (Rubiaceae; Gardeniae fructus) in a ratio of 3:2:2:3 (He et al, 2022a;He et al, 2022b;Liao et al, 2022). As a Chinese classical formula with heat-clearing and detoxicating effects, HLJDD has been proved to have the potential to treat AD by many modern pharmacological studies (Sun et al, 2018;Wu et al, 2020).…”

Rapid separation and identification of 96 main constituents in Huanglian Jiedu decoction via ultra‐high performance liquid chromatography–Orbitrap Fusion Tribrid mass spectrometer

“…The advantages of high separation efficiency, fast scanning speed, high resolution, and high sensitivity significantly improve the ability to rapidly analyze and identify the complex chemical components in TCM. Consequently, UPLC‐Orbitrap Fusion TMS has gradually emerged as a technique for identifying complex components in TCM in recent years 13–15 . In the present study, UPLC‐Orbitrap Fusion TMS is used for the separation and identification of multiple constituents in HLJDD, with the aim of reducing the analytical time and improving the separation resolution.…”

“…Consequently, UPLC-Orbitrap Fusion TMS has gradually emerged as a technique for identifying complex components in TCM in recent years. [13][14][15] In the present study, UPLC-Orbitrap Fusion TMS is used for the separation and identification of multiple constituents in HLJDD, with the aim of reducing the analytical time and improving the separation resolution. Based on a comparison of retention times and TOF-MS/MS data for the analyzed compounds with those of authentic standards, in addition to the published results, we unambiguously identify or tentatively characterize a total of 96 constituents, including 34 flavonoids and their glycosides, 23 alkaloids, 18 organic acids, 5 monoterpenoids, 5 iridoids, 3 diterpenoids, and 8 miscellaneous compounds.…”