Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice

Machado, Juliana Giantomassi; Hoffmann, Juliano Leônidas; Krause, Vicki; Silva, A. V. da; Dias-Melício, Luciane Alarcão; Langoni, Hélio

doi:10.1590/s1678-91992010000100013

Cited by 2 publications

(3 citation statements)

References 12 publications

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“…It presents high levels of endemicity, morbidity, and mortality in populations in Africa, Asia, the Middle East, the Mediterranean region, and Latin America (4) . These diseases are transmitted to humans and other mammals through the bite of the female insect Lutzomyia spp (5) .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract

Correia

Lima

Oliveira

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction: Malaria and leishmaniasis are prevalent in tropical regions, which have environmental characteristics that are highly favorable to protozoa and vectors of these diseases; the transmission of these infections in sub-tropical regions, although recognized, represents only a small fraction of cases. Plants are constantly being used in the search for and acquisition of new drugs, and many compounds derived from them have been used to combat various diseases. In this study, we evaluated the action of the dichloromethanolic extract of Myrciaria dubia leaves against the protozoa Plasmodium falciparum, Leishmania amazonensis, Leishmania braziliensis, and Leishmania chagasi through bioassays. Methods: The extract from M. dubia was tested for its anti-P. falciparum activity in an anti-histidine-rich protein II immunosorbent assay. The antileishmanial assays were performed using the resazurin method, while cytotoxicity against human hepatoma (HepG2) strain was determined using the colorimetric MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide] method. Results: The M. dubia extract presented a half-maximal inhibitory concentration equal to 2.35 (1.05)μg/mL for P. falciparum, 190.73 (6.41) μg/mL for L. amazonensis, and greater than equal to 200µg/mL for L. chagasi and L. braziliensis strains. The cytotoxic concentration for 50% of the cells was above 500μg/mL for HepG2, indicating no toxicity and greater selectivity against parasites. Conclusions:The results obtained indicate the presence of antiplasmodial and leishmanicidal bioactive compounds in the dichloromethanolic extracts of M. dubia leaves, and point towards future studies to elucidate the mechanism of action for each physiological effect.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract

Correia

Lima

Oliveira

et al. 2016

Rev. Soc. Bras. Med. Trop.

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“…infected mice at 24 dpi and the weight of this organ decreased at 16 dpi in immunosuppressed infected mice. Likewise, Machado et al [ 21 ], in a Leishmania chagasi experimental model, observed a decrease in spleen weight in steroid-immunosuppressed mice. The change in mass of organs located in the peritoneal cavity is one of the indicators of the intensity of peritonitis and may also result from increased cell proliferation [ 22 ].…”

Section: Discussionmentioning

confidence: 92%

“…Moreover, Gangneux et al [ 57 ] observed that the percentage of CD4+ and CD8+ T cells in the spleen of infected Leishmania infantum mice treated with glucocorticoids did not show statistical differences vs the control group. Immunosuppressive therapy may interfere with the circulation of immune cells, promote apoptosis of lymphoid cells, and induce downregulation of T cells [ 21 ]. Moreover, in Acanthamoeba spp .…”

Section: Discussionmentioning

confidence: 99%

Changes in the immune system in experimental acanthamoebiasis in immunocompetent and immunosuppressed hosts

et al. 2018

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BackgroundAcanthamoebiasis is most often found in patients with immune deficiency, with infections facilitated by the intake of immunosuppressive drugs. The host immune response to Acanthamoeba spp. infection is poorly understood. Thus, in this study, we aimed to examine the course of Acanthamoeba spp. infection taking into account the host’s immunological status, including assessment of the hematological parameters, cytokine analysis, immunophenotypic changes in spleen populations, and histological spleen changes, which could help clarify some aspects of the immune response to acanthamoebiasis. In our experimental study, we used Acanthamoeba strain AM 22 isolated from the bronchoaspirate of a patient with acute myeloid leukaemia (AML) and atypical pneumonia symptoms.ResultsAcanthamoeba spp. affected the hematological parameters in immunocompetent and immunosuppressed mice and induced a change in spleen weight during infection. Moreover, analysis of anti-inflammatory (IL-4 and IL-10) and pro-inflammatory (IL-17A and IFN-γ) cytokines produced by splenocytes stimulated with concanavalin A demonstrated that Acanthamoeba spp. induced a selective Th1, Th2 and Th17 response at later stages of the infection in immunocompetent hosts. In the case of hosts with low immunity, Acanthamoeba elicited robust Th1 cell-mediated immunity without the participation of Th17. We observed suppression of CD8+ and CD4+ T lymphocytes and CD3+CD4-CD8- double-negative (DN) T lymphocyte populations in the beginning, and in the case of CD3+/CD4+/CD8+ double-positive (DP) T cells in the final phase of Acanthamoeba spp. infection in hosts with low immunity. Also, CD4+T lymphocytes and CD3+/CD4+ and CD3+/CD8+ lymphocyte counts during each stage of acanthamoebiasis were shown to be upregulated.ConclusionsWe demonstrated that analysis of the immune response and pathogenesis mechanisms of clinical isolates of Acanthamoeba spp. in an animal model not only has purely cognitive significance but above all, may help in the development of effective methods of pharmacological therapy especially in patients with low immunity.

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Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice

Cited by 2 publications

References 12 publications

Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract

Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract

Changes in the immune system in experimental acanthamoebiasis in immunocompetent and immunosuppressed hosts

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