Cell-mediated BMP-2 liberation promotes bone formation in a mechanically unstable implant environment

Hägi, Tobias T.; Wu, Gang; Liu, Yuelian; Hunziker, Ernst B.

doi:10.1016/j.bone.2010.02.010

Cited by 23 publications

(20 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, the comparison between the extent of inflammation in topographically different areas such as the skin area compared to the lumbar body area, which are subjected to different biomechanical stress fields [17], and also to different degrees of vascularity (Figure 5 and Figure 6), that both physiologically do occur at these sites, revealed no differences in the extent of the inflammatory response (Figure 3). This basically implies that the degree of vascularity is irrelevant respecting the extent of the acute inflammation response that can be expected following implantation of foreign materials into the body.…”

Section: Discussionmentioning

confidence: 99%

“…ACS carrier material was implanted in the subcutaneous space in the back area (lumbar level). By this set up the deposited collagen carrier patch is exposed on one side towards the skin, where the skin muscles of the rat generate a continuous instability situation, i.e., a high biomechanical instability [17]. On the opposite side of the collagen patch, facing the large underlying lumber muscle package, a relatively stable micromechanical environment is present.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Acute Inflammatory Response to Absorbed Collagen Sponge Is Not Enhanced by BMP-2

Huang

Wismeijer

Hunziker

et al. 2017

IJMS

Self Cite

View full text Add to dashboard Cite

Absorbed collagen sponge (ACS)/bone morphogenetic protein-2 (BMP-2) are widely used in clinical practise for bone regeneration. However, the application of this product was found to be associated with a significant pro-inflammatory response, particularly in the early phase after implantation. This study aimed to clarify if the pro-inflammatory activities, associated with BMP-2 added to ACS, were related to the physical state of the carrier itself, i.e., a wet or a highly dehydrated state of the ACS, to the local degree of vascularisation and/or to local biomechanical factors. ACS (0.8 cm diameter)/BMP-2 were implanted subcutaneously in the back of 12 eight-week-old Sprague Dawley rats. Two days after surgery, the implanted materials were retrieved and analysed histologically and histomorphometrically. The acute inflammatory response following implantation of ACS was dependent of neither the presence or absence of BMP-2 nor the degree of vascularization in the surrounding tissue nor the hydration state (wet versus dry) of the ACS material at the time of implantation. Differential micro biomechanical factors operating at the implantation site appeared to have an influence on the thickness of inflammation. We conclude that the degree of the early inflammatory response of the ACS/BMP-2 may be associated with the physical and chemical properties of the carrier material itself.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Acute Inflammatory Response to Absorbed Collagen Sponge Is Not Enhanced by BMP-2

Huang

Wismeijer

Hunziker

et al. 2017

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Similarly, BMP2 was incorporated into biomimetic coatings of calcium phosphate (CaP) on porous scaffolds, so that its release was controlled by osteoclast degradation of the coating [83–85]. Titanium alloy discs were immersed in concentrated simulated body fluid to inhibit crystal growth, resulting in the formation of a thin layer of amorphous CaP that could serve as a seeding surface for the subsequent addition of a crystalline CaP layer containing BMP2 [83].…”

Section: Advantages Of Cr Systems For Tissue Engineeringmentioning

confidence: 99%

“…Interestingly, in this orthotopic model, the presence of BMP2 caused an overall decrease in new bone volume, attributed to an overly high dose (13 and 23µg/implant) that stimulated bone resorption activities of osteoclasts. With a lower dose (5.8µg), the sustained release of BMP2 caused enhanced bone formation compared to burst release in a subcutaneous implantation model in rat, even in a mechanically unstable situation that normally inhibits osteogenesis [85]. …”

Section: Advantages Of Cr Systems For Tissue Engineeringmentioning

confidence: 99%

Clinical translation of controlled protein delivery systems for tissue engineering

Spiller

Vunjak-Novakovic

2013

Drug Deliv. and Transl. Res.

View full text Add to dashboard Cite

Strategies that utilize controlled release of drugs and proteins for tissue engineering have enormous potential to regenerate damaged organs and tissues. The multiple advantages of controlled release strategies merit overcoming the significant challenges to translation, including high costs and long, difficult regulatory pathways. This review highlights the potential of controlled release of proteins for tissue engineering and regenerative medicine. We specifically discuss treatment modalities that have reached preclinical and clinical trials, with emphasis on controlled release systems for bone tissue engineering, the most advanced application with several products already in clinic. Possible strategies to address translational and regulatory concerns are also discussed.

show abstract

“…The biological and biomechanical connotation of bone formation by direct rather than endochondral ossifi cation is well established. 38 This pattern of bone formation is known to occur only when there is a stable mechanical environment that is devoid of shear stress as well as availability of sufficient volume of potent osteoinductive agent. 39 By inference, the use of bone screws and rods combined with the relatively fl exible mPCL/TCP scaffolds provided adequate stability for fusion in this quadruped model of interbody fusion.…”

Section: ➢ Key Pointsmentioning

confidence: 99%

Fusion Performance of Low-Dose Recombinant Human Bone Morphogenetic Protein 2 and Bone Marrow-Derived Multipotent Stromal Cells in Biodegradable Scaffolds

Abbah¹,

Lam²,

Ramruttun³

et al. 2011

Spine

View full text Add to dashboard Cite

mPCL/TCP scaffolds loaded with low-dose rhBMP-2 is comparable to autograft bone as a bone graft substitute in this large animal ALIF model. Although BMSCs lagged behind autograft bone and rhBMP-2, evidence of bone ingrowth in this group warrants further investigation. Our results suggest that mPCL/TCP scaffolds loaded with rhBMP-2 or BMSCs may be a viable alternative to conventional cages and autograft bone.

show abstract

Cell-mediated BMP-2 liberation promotes bone formation in a mechanically unstable implant environment

Cited by 23 publications

References 20 publications

The Acute Inflammatory Response to Absorbed Collagen Sponge Is Not Enhanced by BMP-2

The Acute Inflammatory Response to Absorbed Collagen Sponge Is Not Enhanced by BMP-2

Clinical translation of controlled protein delivery systems for tissue engineering

Fusion Performance of Low-Dose Recombinant Human Bone Morphogenetic Protein 2 and Bone Marrow-Derived Multipotent Stromal Cells in Biodegradable Scaffolds

Contact Info

Product

Resources

About