Cell-Mediated and IgE-Mediated Immune Responses in Atopic Dermatitis

Clark, Richard A.F.

doi:10.1001/archderm.1989.01670150103018

Cited by 26 publications

(2 citation statements)

References 60 publications

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“…Immunoglobulin-E plays a role in allergen-induced inflammatory processes in atopic subjects via preformed allergen-specific IgE molecules bounded to the high-affinity IgE receptors (FcεRI) on the surfaces of various immune cells [ 2 , 3 ]. By binding with incoming allergens, IgE acts as an effector for activation of immune cells, chemical mediator release and cytokine/chemokine production.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Pro-Inflammatory and Pruritogenic Transcriptome in Skin Lesions of the Experimental Canine Atopic Acute IgE-Mediated Late Phase Reactions Model and Correlation to Acute Skin Lesions of Human Atopic Dermatitis

Blubaugh,

Hoover,

Kim

et al. 2024

Veterinary Sciences

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Intradermal injection of anti-immunoglobulin E (IgE) antibodies in dogs grossly and histologically resemble naturally occurring atopic dermatitis (AD). However, the activated inflammatory and pruritic pathways have not been characterized. The objectives of this study were to characterize the inflammatory transcriptome of experimental acute canine IgE-induced lesions and to determine how these correlate to the transcriptome of naturally occurring human and canine acute atopic dermatitis. Biopsies were collected at 6 and 24 h after intradermal injections of anticanine-IgE antibodies to eight healthy male castrated Beagles; healthy and saline-injected skin served as controls. We extracted total RNA from skin biopsies and analyzed transcriptome using RNA-sequencing. Gene expressions of IgE-induced biopsies were compared to that of controls from the same subject (1.5-fold change, p-adjusted value ≤ 0.05). Acute IgE-mediated lesions had a significant upregulation of pro-inflammatory (e.g., LTB, IL-1B, PTX3, CCL2, IL6, IL8, IL18), T helper-(Th)1/IFNγ signal (e.g., STAT-1, OASL, MX-1, CXCL10, IL-12A) and Th2 (e.g., IL4R, IL5, IL13, IL33 and POSTN) genes, as well as Th2 chemokines (CCL17, CCL24). Pathway analysis revealed strong significant upregulation of JAK-STAT, histamine, IL-4 and IL13 signaling. Spearman correlation coefficient for the shared DEGs between canine anti-canine-IgE and human AD samples revealed a significant moderate positive correlation for anti-canine-IgE 6-h samples (r = 0.53) and 24-h samples (r = 0.47). In conclusion, acute canine IgE-mediated skin lesions exhibit a multipolar immunological axis upregulation (Th1, Th2 and Th17) in healthy dogs, resembling acute spontaneous human AD lesions.

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Section: Introductionmentioning

confidence: 99%

Characterization of the Pro-Inflammatory and Pruritogenic Transcriptome in Skin Lesions of the Experimental Canine Atopic Acute IgE-Mediated Late Phase Reactions Model and Correlation to Acute Skin Lesions of Human Atopic Dermatitis

Blubaugh,

Hoover,

Kim

et al. 2024

Veterinary Sciences

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show abstract

“…In contrast many authors have shown that application of patch tests, solutions or ointments containing a variety of aeroallergens (house dust mite, tree, grass and weed pollens, animal danders and molds) to the skin of patients with atopic dermatitis results in the clinical and histological features of an acute eczema.12-'5 Both the time course and the cellular infiltrate have suggested that a form of delayed hypersensitivity had occurred. [6][7][8][9][10][11][12][13][14][15][16][17] Percutaneous entry of applied aeroallergen could allow binding to the increased numbers of antigen presenting epidermal Langerhans cells in the skin of patients with atopic dermatitis. 18 Recently, IgE has been identified on the surface of cutaneous dendritic cells (principally Langerhans cells and interdigitating reticulum cells) in both lesional and nonlesional skin of patients with active atopic dermatitis.19'20 Therefore the capacity of antigen trapping via the surface bound IgE and the ability of Langerhans cells to induce T lymphocyte activation and the generation of cytotoxic T lymphocytes could be the initiating events in the delayed hypersensitivity reaction.…”

Section: Introductionmentioning

confidence: 99%