Cell-material interactions in tendon tissue engineering

Lin, Junxin; Zhou, Wenyan; Han, Shan; Bunpetch, Varitsara; Zhao, Kun; Liu, Chaozhong; Yin, Zi; Ouyang, Hongwei

doi:10.1016/j.actbio.2018.01.012

Cited by 76 publications

(54 citation statements)

References 143 publications

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“…[28][29][30] International Journal of Nanomedicine downloaded from https://www.dovepress.com/ by 44.224.250 Tremendous progress has been made in research involving tendon to bone healing using a combination of biomaterials, cells, and growth factors. 6,[31][32][33] In the clinic, the surgically repaired tendon to bone construct is connected by scar formation. The percentage of patients with failure after tendon to bone repair is relatively high.…”

Section: Discussionmentioning

confidence: 99%

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

Su¹,

Wang²,

Jiang³

et al. 2019

IJN

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Background These normal entheses are not reestablished after repair despite significant advances in surgical techniques. There is a significant need to develop integrative biomaterials, facilitating functional tendon-to-bone integration. Materials and methods We fabricated a highly interconnective graphene oxide-doped electrospun poly(lactide-co-glycolide acid) (GO-PLGA) nanofibrous membrane by electrospinning technique and evaluated them using in vitro cell assays. Then, we established rabbit models, the PLGA and GO-PLGA nanofibrous membranes were used to augment the rotator cuff repairs. The animals were killed postoperatively, which was followed by micro-computed tomography, histological and biomechanical evaluation. Results GO was easily mixed into PLGA filament without changing the three dimensional microstructure. An in vitro evaluation demonstrated that the PLGA membranes incorporated with GO accelerated the proliferation of BMSCs and furthered the Osteogenic differentiation of BMSCs. In addition, an in vivo assessment further revealed that the local application of GO-PLGA membrane to the gap between the tendon and the bone in a rabbit model promoted the healing enthesis, increased new bone and cartilage generation, and improved collagen arrangement and biomechanical properties in comparison with repair with PLGA only. Conclusion The electrospun GO-PLGA fibrous membrane provides an effective approach for the regeneration of tendon to bone enthesis.

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Section: Discussionmentioning

confidence: 99%

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

Su¹,

Wang²,

Jiang³

et al. 2019

IJN

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“…Decades of research on tendon injuries and regeneration has provided a basic understanding of the biological and mechanical functions of tendons required to maintain posture and generate motion. Nevertheless, tendinopathy and tendon injury still remain a clinical challenge due to their poor self‐healing capacity, limited cellularity, vascularity, and innervation (Hart, Kydd, & Reno, ; Lin et al, ). TSCs play the vital role for tendinopathy healing with its tenogenic differentiation, differentiating into tenocytes and extracellular matrix formation.…”

Section: Discussionmentioning

confidence: 99%

Aspirin promotes tenogenic differentiation of tendon stem cells and facilitates tendinopathy healing through regulating the GDF7/Smad1/5 signaling pathway

Wang

Tang

et al. 2019

Journal Cellular Physiology

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Tendinopathy is a common musculoskeletal system disorder in sports medicine, but regeneration ability of injury tendon is limited. Tendon stem cells (TSCs) have shown the definitive treatment evidence for tendinopathy and tendon injuries due to their tenogenesis capacity. Aspirin, as the representative of nonsteroidal anti-inflammatory drugs for its anti-inflammatory and analgestic actions, has been commonly used in treating tendinopathy in clinical, but the effect of aspirin on tenogenesis of TSCs is unclear. We hypothesized that aspirin could promote injury tendon healing through inducing TSCs tenogenesis. The aim of the present study is to make clear the effect of aspirin on TSC tenogenesis and tendon healing in tendinopathy, and thus provide new treatment evidence and strategy of aspirin for clinical practice. First, TSCs were treated with aspirin under tenogenic medium for 3, 7, and 14 days. Sirius Red staining was performed to observe the TSC differentiation. Furthermore, RNA sequencing was utilized to screen out different genes between the induction group and aspirin treatment group. Then, we identified the filtrated molecules and compared their effect on tenogenesis and related signaling pathway. At last, we constructed the tendinopathy model and compared biomechanical changes after aspirin intake.From the results, we found that aspirin promoted tenogenesis of TSCs. RNA sequencing showed that growth differentiation factor 6 (GDF6), GDF7, and GDF11were upregulated in induction medium with the aspirin group compared with the induction medium group. GDF7 increased tenogenesis and activated Smad1/5 signaling. In addition, aspirin increased the expression of TNC, TNMD, and Scx and biomechanical properties of the injured tendon. In conclusion, aspirin promoted TSC tenogenesis and tendinopathy healing through GDF7/Smad1/5 signaling, and this provided new treatment evidence of aspirin for tendinopathy and tendon injuries. K E Y W O R D S NSAIDs, stem cell differentiation, tendon injury healing SUPPORTING INFORMATION Additional supporting information may be found online in the Supporting Information section. How to cite this article: Wang Y, He G, Tang H, et al. Aspirin promotes tenogenic differentiation of tendon stem cells and facilitates tendinopathy healing through regulating the GDF7/Smad1/5 signaling pathway. J Cell Physiol. 2020;235: 4778-4789. https://doi.

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“…There is a need in tendon tissue engineering to establish an optimal environment to promote tenogenic differentiation and healing [2,30]. In this case, suitable material selection for manufacturing of a tendon scaffold is necessary for effective treatment of tendon injuries [30].…”

Section: Discussionmentioning

confidence: 99%

“…Damage to the Achilles tendon is a universal health problem resulting from poor healing of the tendon tissue. For these reasons, many bioengineering researchers have investigated biomaterials as possible therapeutic options for tendon tissue repair [2].…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo assessments of an optimal polyblend composition of polycaprolactone/gelatin nanofibrous scaffolds for Achilles tendon tissue engineering

Lee

Kim

Heo

et al. 2019

Journal of Industrial and Engineering Chemistry

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In this study, we manufactured various ratios of polycaprolactone (PCL)/gelatin (GE) highly aligned electrospun nanofibrous scaffolds (ENs) to investigate the effects of polymer ratio on tenogenic differentiation activity. For biological assessments, the cell proliferation rate was optimal in the PCL/GE (9:1) group. Interestingly, however, the tenogenic differentiation rate was best for the PCL/GE (7:3) group. From our outcomes, we established that a poly-blending mix of PCL/GE (7:3) is a promising ratio for tenogenic differentiation. Thus, our findings may provide for an effective mesh to promote tenogenic differentiation of ENs in future tendon tissue engineering applications.

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Cell-material interactions in tendon tissue engineering

Cited by 76 publications

References 143 publications

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

Aspirin promotes tenogenic differentiation of tendon stem cells and facilitates tendinopathy healing through regulating the GDF7/Smad1/5 signaling pathway

In vitro and in vivo assessments of an optimal polyblend composition of polycaprolactone/gelatin nanofibrous scaffolds for Achilles tendon tissue engineering

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