Cell-laden microfibers for bottom-up tissue engineering

Onoe, Hiroaki; Takeuchi, Shoji

doi:10.1016/j.drudis.2014.10.018

Cited by 129 publications

(110 citation statements)

References 77 publications

Supporting

Mentioning

110

Contrasting

Order By: Relevance

“…To be able to control the cellular distribution and physical properties, cell-laden fibers should be used during the assembly process [5]. Among different biomaterials used as cell-carriers, hydrogels, which are 3D polymeric networks with high water content, provide a nurturing environment for cellular growth and maturation [6].…”

Section: Fabricating Cell-laden Fibersmentioning

confidence: 99%

Textile Processes for Engineering Tissues with Biomimetic Architectures and Properties

Fallahi

Khademhosseini

Tamayol

2016

Trends in Biotechnology

View full text Add to dashboard Cite

show abstract

Section: Fabricating Cell-laden Fibersmentioning

confidence: 99%

Textile Processes for Engineering Tissues with Biomimetic Architectures and Properties

Fallahi

Khademhosseini

Tamayol

2016

Trends in Biotechnology

View full text Add to dashboard Cite

show abstract

“…Recently, a new concept of bottom‐up (BU) strategy has emerged wherein the cell‐laden modular microtissues (MTs) are first built and then assembled into macroscopic tissues by random packing or microfluidics, etc . These MTs can be fabricated in many forms, including self‐assembled cellular aggregates, cell‐laden microgels or microcarriers, and creation of cell sheets .…”

Section: Introductionmentioning

confidence: 99%

An in vivo comparative study of the gelatin microtissue‐based bottom‐up strategy and top‐down strategy in bone tissue engineering application

Luo

Fang

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Tissue‐engineered bone grafts (TEBGs) represent a promising treatment for bone defects. Nevertheless, drawbacks of the current construction strategy (top‐down [TD] strategy) such as limited transmission of nutrients and nonuniform distribution of seeded cells, result in an unsatisfied therapeutic effect on large segmental bone defects. Theoretically, tissue‐engineered microtissue (TEMT)‐based bottom‐up (BU) strategy is effective in preserving seed cells and vascularization, thus being regarded as a better alternative for TEBGs. Yet, there are few studies focusing on the comparison of the in vivo performance of TEBGs fabricated by TD or BU strategy. Here, we developed an ectopic bone formation rat model to compare the performance of these two construction strategies in vivo. TEBGs made from gelatin TEMT (BU strategy) and bulk tissue (BT; TD strategy) were seeded with equal number of rat bone marrow‐derived mesenchymal stem cells and fabricated in 5 mm polydimethylsiloxane chambers. The grafts were implanted into subcutaneous pockets in the same rat. Four weeks after implantation, microcomputed tomography and hematoxylin and eosin staining results demonstrated that more bony tissue was formed in the microtissue (MT) group than in the BT group. CD31 staining further confirmed that there were more blood vessels in the MT group, indicating that the BU strategy was superior in inducing angiogenesis. This comparative study provides evidence that the BU construction strategy is more effective for in vivo application and bone defect treatment by bone tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 678–688, 2019.

show abstract

“…[22,23,37,38] We used three types of neural cells to fabricate microfiber-shaped neural tissues: primary mouse neural stem cells (mNSCs) dissected from the midbrain of mice (mNSC units), primary cortical cells (cortical units), and primary hippocampal cells (hippocampal units). To prepare the rod-shaped neural units in an accurate and reproducible manner, we developed a fiber-cutting device that can fix the microfiber-shaped neural tissues in a culture In the brain, 3D neural networks such as the hippocampalprefrontal pathway, [1,2] amygdala pathway, [3][4][5] and visual pathway [3,6,7] comprise various independent regions that are connected by aligned nerve fibers.…”

Section: Rod-shaped Neural Units For Aligned 3d Neural Network Connecmentioning

confidence: 99%

Rod‐Shaped Neural Units for Aligned 3D Neural Network Connection

Kato-Negishi

Onoe

Ito

et al. 2017

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

COMMUNICATION (1 of 7)© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Rod-Shaped Neural Units for Aligned 3D Neural Network ConnectionMidori Kato-Negishi, Hiroaki Onoe, Akane Ito, and Shoji Takeuchi* DOI: 10.1002/adhm.201700143 guidance [12,26] have also been developed as alternative means of controlling cell patterning with high cell density and/or aligned nerve fibers. However, coculturing different types of neural tissues is still difficult because culture conditions such as medium, growth factors, and extracellular matrix (ECM) differ according to the type of neural tissues. [27][28][29][30][31][32][33] Neurons also have the characteristic of axon overproduction and, consequently, axons extend over a wide area during early development. [34][35][36] Therefore, selective connectivity between specific, spatially separated neural tissues is technically difficult.This paper proposes neural tissue units with aligned nerve fibers, called "rodshaped neural units," that can connect spatially separate neural regions (Figure 1). To fabricate these units, we employ a meterlong microfiber-shaped neural tissue [22,23] as starting material, in which stem-cell-derived or primary neural cells are encapsulated and aligned within an alginate tubular hydrogel. This tissue is cut into millimeter-sized sections and, after further cell culturing, both edges of the sections bulge out and form spherical ends (Figure 1b). The proposed rod-shaped neural units have the following characteristics: (1) nerve fibers are aligned in the longitudinal direction of the rod, (2) each unit has an insulated region covered with a thin alginate hydrogel layer to prevent the encapsulated neural tissues from connecting to surrounding cells within the regions, and (3) the edges of the unit are connectable, with the spherical ends functioning as glue to connect with other neural tissues or units. In this study, we designed and fabricated the rod-shaped neural units and characterized their connectivity in terms of neuronal morphologies and network formation. In addition, we constructed a neural network from different types of neural tissues in different culture conditions, with connections to specific, spatially neural regions. Therefore, our neural units assembly can coculture different types of neural tissues with aligned nerve fibers that recapitulate the topological complexity of in vivo neural networks with high cell density and cell population.Rod-shaped neural units were prepared by cutting microfibershaped neural tissues, as reported previously. [22,23,37,38] We used three types of neural cells to fabricate microfiber-shaped neural tissues: primary mouse neural stem cells (mNSCs) dissected from the midbrain of mice (mNSC units), primary cortical cells (cortical units), and primary hippocampal cells (hippocampal units). To prepare the rod-shaped neural units in an accurate and reproducible manner, we developed a fiber-cutting device that can fix the microfiber-shaped neural tissues in a culture In the brain, 3D neural networks such as the hippoc...

show abstract

Cell-laden microfibers for bottom-up tissue engineering

Cited by 129 publications

References 77 publications

Textile Processes for Engineering Tissues with Biomimetic Architectures and Properties

Textile Processes for Engineering Tissues with Biomimetic Architectures and Properties

An in vivo comparative study of the gelatin microtissue‐based bottom‐up strategy and top‐down strategy in bone tissue engineering application

Rod‐Shaped Neural Units for Aligned 3D Neural Network Connection

Contact Info

Product

Resources

About