Textile Processes for Engineering Tissues with Biomimetic Architectures and Properties

Fallahi, Afsoon; Khademhosseini, Ali; Tamayol, Ali

doi:10.1016/j.tibtech.2016.07.001

Cited by 32 publications

(25 citation statements)

References 13 publications

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“…Such fibrous constructs can potentially be engineered and generated using various fiber-forming techniques and textile processes [12, 13]. Traditional strategies for creating fibrous scaffolds include two steps, in which micrometer-scale fibers are generated through the utilization of wet spinning and dry spinning, and then are assembled into 2D or 3D constructs with tunable properties by textile technology [14].…”

Section: Introductionmentioning

confidence: 99%

Living nanofiber yarn-based woven biotextiles for tendon tissue engineering using cell tri-culture and mechanical stimulation

et al. 2017

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Non-woven nanofibrous scaffolds have been developed for tendon graft application by using electrospinning strategies. However, electrospun nanofibrous scaffolds face some obstacles and limitations, including suboptimal scaffold structure, weak tensile and suture-retention strengths, and compact structure for cell infiltration. In this work, a novel nanofibrous, woven biotextile, fabricated based on electrospun nanofiber yarns, was implemented as a tissue engineered tendon scaffold. Based on our modified electrospinning setup, polycaprolactone (PCL) nanofiber yarns were fabricated with reproducible quality, and were further processed into plain-weaving fabrics interlaced with polylactic acid (PLA) multifilaments. Nonwoven nanofibrous PCL meshes with random or aligned fiber structures were generated using typical electrospinning as comparative counterparts. The woven fabrics contained 3D aligned microstructures with significantly larger pore size and obviously enhanced tensile mechanical properties than their nonwoven counterparts. The biological results revealed that cell proliferation and infiltration, along with the expression of tendon-specific genes by human adipose derived mesenchymal stem cells (HADMSC) and human tenocytes (HT), were significantly enhanced on the woven fabrics compared with those on randomly-oriented or aligned nanofiber meshes. Co-cultures of HADMSC with HT or human umbilical vein endothelial cells (HUVEC) on woven fabrics significantly upregulated the functional expression of most tenogenic markers. HADMSC/HT/HUVEC tri-culture on woven fabrics showed the highest upregulation of most tendon-associated markers than all the other mono- and co-culture groups. Furthermore, we conditioned the tri-cultured constructs with dynamic conditioning and demonstrated that dynamic stretch promoted total collagen secretion and tenogenic differentiation. Our nanofiber yarn-based biotextiles have significant potential to be used as engineered scaffolds to synergize the multiple cell interaction and mechanical stimulation for promoting tendon regeneration.

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Section: Introductionmentioning

confidence: 99%

Living nanofiber yarn-based woven biotextiles for tendon tissue engineering using cell tri-culture and mechanical stimulation

et al. 2017

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“…Various tissue engineering applications have already used different hydrogels such as hyaluronic acid, gelatin, chondroitin sulfate, and alginate due to their high water binding capacity, porous structure, and tunable degradation properties . Furthermore, hydrogels have a great potential to be shaped into various morphologies using wide range of fabrication techniques such as 3D printing, wet spinning, and weaving . In recent years, among hydrogels used in biomedical applications, gelatin methacrylate (GelMA) hydrogel became an attractive candidate due to gelatin's favorable biocompatible characteristics, and because it can be photocrosslinked to form a stable gel at body temperature (37°C) .…”

Section: Introductionmentioning

confidence: 99%

Gelatin methacrylate scaffold for bone tissue engineering: The influence of polymer concentration

Celikkin

Mastrogiacomo

Jaroszewicz

et al. 2017

J Biomedical Materials Res

134

110

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Gelatin methacrylate (GelMA) is an inexpensive, photocrosslinkable, cell-responsive hydrogel which has drawn attention for a wide range of tissue engineering applications. The potential of GelMA scaffolds was demonstrated to be tunable for different tissue engineering (TE) applications through modifying the polymer concentration, methacrylation degree, or UV light intensity. Despite the promising results of GelMA hydrogels in tissue engineering, the influence of polymer concentration for bone tissue engineering (BTE) scaffolds was not established yet. Thus, in this study, we have demonstrated the effect of polymer concentration in GelMA scaffolds on osteogenic differentiation. We prepared GelMA scaffolds with 5 and 10% polymer concentrations and characterized the scaffolds in terms of porosity, pore size, swelling characteristics, and mechanical properties. Subsequent to the scaffolds characterization, the scaffolds were seeded with bone marrow derived rat mesenchymal stem cells and cultured in osteogenic media to evaluate the possible osteogenic differentiation effect exerted by the polymer concentration. After 7, 14, 21, and 28 days, DNA content, calcium deposition, and alkaline phosphatase (ALP) activity of scaffolds were evaluated quantitatively by colorimetric bioassays. Furthermore, the distribution of the calcium deposition within the scaffolds was attained qualitatively and quantitatively by microcomputer tomography (mCT). Our data suggest that GelMA hydrogels prepared with 5% polymer concentration has promoted homogeneous extracellular matrix calcification and it is a great candidate for BTE applications.

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“…These properties are well aligned with the expectations of ideal scaffolds for tissue engineering applications [82–85]. In addition, the similarity between textile-based fabrics and some tissues, such as muscle, tendon, and ligament, has fueled the interest for their use in tissue engineering [86]. Thus, textile technology based on weaving, braiding, and knitting has been used for the engineering of various tissue constructs [87].…”

Section: Spatial Control Of Hydrogelsmentioning

confidence: 79%

“…The main methods used for fabrication of cell-laden fibers include wet-spinning, microfluidic spinning, and interfacial complexation [86]. In the case of wet-spinning, the prepolymer is injected into a reservoir containing compounds and the formation of stable fibers requires a rapid crosslinking rate of the prepolymers to limit the undesired diffusion into the surrounding solution.…”

Section: Spatial Control Of Hydrogelsmentioning

confidence: 99%

Spatially and temporally controlled hydrogels for tissue engineering

Leijten

Seo

Yue

et al. 2017

Materials Science and Engineering: R: Reports

Self Cite

152

129

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Summary Recent years have seen tremendous advances in the field of hydrogel-based biomaterials. One of the most prominent revolutions in this field has been the integration of elements or techniques that enable spatial and temporal control over hydrogels’ properties and functions. Here, we critically review the emerging progress of spatiotemporal control over biomaterial properties towards the development of functional engineered tissue constructs. Specifically, we will highlight the main advances in the spatial control of biomaterials, such as surface modification, microfabrication, photo-patterning, and three-dimensional (3D) bioprinting, as well as advances in the temporal control of biomaterials, such as controlled release of molecules, photocleaving of proteins, and controlled hydrogel degradation. We believe that the development and integration of these techniques will drive the engineering of next-generation engineered tissues.

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Textile Processes for Engineering Tissues with Biomimetic Architectures and Properties

Cited by 32 publications

References 13 publications

Living nanofiber yarn-based woven biotextiles for tendon tissue engineering using cell tri-culture and mechanical stimulation

Living nanofiber yarn-based woven biotextiles for tendon tissue engineering using cell tri-culture and mechanical stimulation

Gelatin methacrylate scaffold for bone tissue engineering: The influence of polymer concentration

Spatially and temporally controlled hydrogels for tissue engineering

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