Cell Inactivation and DNA Single- and Double-strand Breaks in Cultured Mammalian Cells Irradiated by a Thermal Neutron Beam

Maki, Hirotoshi; Saito, Masahiro; Kobayashi, Tooru; Kawai, Ken‐ichi; Akaboshi, Mitsuhiko

doi:10.1080/09553008614551231

Cited by 19 publications

(19 citation statements)

References 26 publications

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“…Data from two groups where repair of dsb induced by y-irradiation or neutrons was compared using neutral filter elution showed no apparent difference in the rate of repair, or indeed the extent of dsb repair ( Van der Schans et al . 1983, Maki et al . 1986) .…”

Section: Discussionmentioning

confidence: 95%

The Relationship between Radiation-induced DNA Double-strand Breaks and Cell Kill in Hamster V79 Fibroblasts Irradiated with 250 kVp X-rays, 2·3 MeV Neutrons or²³⁸Pu α-particles

Prise

Davies

Michael

1987

International Journal of Radiation Biology and Related Studies

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Using the neutral filter elution technique, the induction of DNA double-strand breaks (dsb) has been measured in 250 kVp X-irradiated V79-379A Chinese hamster cells irradiated under air or nitrogen. The dose-effect curves for induced dsb were curvilinear, mirroring cell survival curves, such that there was an approximately linear relationship between induced dsb and lethal lesions (-In (cell survival)) which was independent of oxygen. With cells irradiated with 2.3 MeV neutrons or 238Pu alpha-particles the correlations between lethal events and dsb, although also approximately linear, do not match those for X-rays. With neutrons there is approximately a 2.5-fold reduction in the level of dsb induction per lethal event. Thus either the apparently linear relationships found are spurious, and there is no general correlation between induced dsb and lethal effect, or there are qualitative differences between neutron, alpha-particle and X-ray induced dsb that give them differing probabilities of cell kill.

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Section: Discussionmentioning

confidence: 95%

The Relationship between Radiation-induced DNA Double-strand Breaks and Cell Kill in Hamster V79 Fibroblasts Irradiated with 250 kVp X-rays, 2·3 MeV Neutrons or²³⁸Pu α-particles

Prise

Davies

Michael

1987

International Journal of Radiation Biology and Related Studies

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“…There have been some reports on the relationship between the ubiquitin-proteasome system and DNA repair. Maki et al reported that thermal neutrons induced single-and double-stranded breaks in DNA in cultured mammalian cells [68]. In the present study, we found that nine genes involved in DNA damage response were among the genes induced by thermal neutron IR.…”

Section: Discussionmentioning

confidence: 72%

Genome-Wide Expression Changes in Saccharomyces cerevisiae in Response to High-LET Ionizing Radiation

Mizukami-Murata

Iwahashi

Kimura

et al. 2010

Appl Biochem Biotechnol

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To understand the yeast response to high-linear energy transfer (LET) ionizing radiation (IR), we investigated global gene expression in yeast irradiated by three types of high-LET IR (fast neutrons, heavy ions, and thermal neutrons) and gamma rays using DNA microarray analysis. Stationary cells were irradiated by each IR and recultured in yeast-peptone-dextrose medium to allow repair for 40 min. RNA was then isolated from three independent samples of irradiated yeast. Genes involved in the Mec1p kinase pathway, which functions in DNA damage response, were induced by all forms of high-LET IR and by gamma rays. Some genes related to oxidative stress and the cell wall were induced by all forms of high-LET IRs. Gene expression patterns as a function of each type of high-LET IR were examined statistically by one-way analysis of variance. This analysis demonstrated the existence of irradiation-specific responses. For example, genes involved in ribosomal DNA synthesis were specifically induced by fast neutron irradiation, while the ubiquitin-proteasome system and heat shock response were specifically induced by thermal neutron irradiation. The study characterizes high-LET IR-induced gene expression and provides a molecular understanding of subsequent adaptation in yeast.

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“…(1983) compared residual breaks after gamma irradiation and 14 . 6 MeV neutron irradiation (< 10 per cent remaining in both cases), and Maki et al (1986) showed about 5 per cent of ssb remaining after 60 min repair time following gamma rays or neutrons from a thermal neutron beam . In contrast, Furuno et al .…”

Section: Resultsmentioning

confidence: 97%

DNA Damage and Repair in Rodent and Human Cells after Exposure to JANUS Fission Spectrum Neutrons: A Minor Fraction of Single-strand Breaks as Revealed by Alkaline Elution is Refractory to Repair

Peak¹,

Peak²,

Carnes³

et al. 1989

International Journal of Radiation Biology

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We have examined the induction and repair of breaks induced in the DNA of Chinese hamster V79 and human P3 epithelial teratocarcinoma cells by JANUS fission-spectrum neutrons (mean energy 0.85 MeV) and 60Co gamma radiation in the biological dose range, using alkaline filter elution methods. Fission-spectrum neutrons induce fewer immediate single-strand breaks (ssb) per gray of absorbed dose than do gamma rays, as measured by alkaline elution methods. Previous survival measurements have indicated incomplete recovery after neutron exposures. The present data demonstrate that whereas most ssb caused by exposure to fission-spectrum neutrons can be rapidly repaired by both cell lines, a small but statistically significant fraction of the ssb induced by exposure to 6 Gy of neutrons is refractory to repair. In contrast, all measurable ssb induced by 3 Gy gamma rays are rapidly repaired.

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Cell Inactivation and DNA Single- and Double-strand Breaks in Cultured Mammalian Cells Irradiated by a Thermal Neutron Beam

Cited by 19 publications

References 26 publications

The Relationship between Radiation-induced DNA Double-strand Breaks and Cell Kill in Hamster V79 Fibroblasts Irradiated with 250 kVp X-rays, 2·3 MeV Neutrons or²³⁸Pu α-particles

The Relationship between Radiation-induced DNA Double-strand Breaks and Cell Kill in Hamster V79 Fibroblasts Irradiated with 250 kVp X-rays, 2·3 MeV Neutrons or²³⁸Pu α-particles

Genome-Wide Expression Changes in Saccharomyces cerevisiae in Response to High-LET Ionizing Radiation

DNA Damage and Repair in Rodent and Human Cells after Exposure to JANUS Fission Spectrum Neutrons: A Minor Fraction of Single-strand Breaks as Revealed by Alkaline Elution is Refractory to Repair

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Cell Inactivation and DNA Single- and Double-strand Breaks in Cultured Mammalian Cells Irradiated by a Thermal Neutron Beam

Cited by 19 publications

References 26 publications

The Relationship between Radiation-induced DNA Double-strand Breaks and Cell Kill in Hamster V79 Fibroblasts Irradiated with 250 kVp X-rays, 2·3 MeV Neutrons or238Pu α-particles

The Relationship between Radiation-induced DNA Double-strand Breaks and Cell Kill in Hamster V79 Fibroblasts Irradiated with 250 kVp X-rays, 2·3 MeV Neutrons or238Pu α-particles

Genome-Wide Expression Changes in Saccharomyces cerevisiae in Response to High-LET Ionizing Radiation

DNA Damage and Repair in Rodent and Human Cells after Exposure to JANUS Fission Spectrum Neutrons: A Minor Fraction of Single-strand Breaks as Revealed by Alkaline Elution is Refractory to Repair

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The Relationship between Radiation-induced DNA Double-strand Breaks and Cell Kill in Hamster V79 Fibroblasts Irradiated with 250 kVp X-rays, 2·3 MeV Neutrons or²³⁸Pu α-particles

The Relationship between Radiation-induced DNA Double-strand Breaks and Cell Kill in Hamster V79 Fibroblasts Irradiated with 250 kVp X-rays, 2·3 MeV Neutrons or²³⁸Pu α-particles