Cell growing on ion implanted polytetrafluorethylene

Kondyurina, Irina; Шардаков, И. Н.; Nechitailo, Galina S.; Terpugov, V. N.; Kondyurin, Alexey

doi:10.1016/j.apsusc.2014.07.057

Cited by 14 publications

(9 citation statements)

References 38 publications

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“…Various surface modification techniques have been employed to guide the cellular responses to PTFE materials. Most of these are chemical modifications that use ion irradiation, plasma irradiation, and UV light irradiation either to introduce amine and oxygen containing functionalities to the material surface or to apply bioactive coatings such as RGD peptides, proteins, antibodies, and heparin . These modifications have been shown to improve the outcome of PTFE biomaterials in terms of endothelialization, neointimal hyperplasia, and thrombosis.…”

Section: Discussionmentioning

confidence: 99%

Polytetrafluoroethylene topographies determine the adhesion, activation, and foreign body giant cell formation of macrophages

Lamichhane

Anderson

Vierhout

et al. 2017

J Biomedical Materials Res

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Polytetrafluoroethylene (PTFE) is one of the commonly used materials in making various cardiovascular implants. However, the success rates of these implants in several occasions are hindered by unwanted immune responses from immune cells, such as macrophages. In this study, we investigated the response of macrophages with different structures (flat, expanded, and electrospun) of PTFE having varied surface topographies: smooth planar surface (flat PTFE), node-fibrils (ePTFE), and randomly oriented microfibers (electrospun PTFE). The electrospun PTFE showed the least adhesion of macrophages. Also, the morphology of macrophages adhered on electrospun PTFE exhibited minimal activation. The macrophage pro-inflammatory cytokine secretions showed that the lowest level of TNF-α was produced on electrospun PTFE whereas IP-10 was produced in lowest levels on expanded PTFE (ePTFE). The production of IL-6 and MCP-1 cytokines was also dependent on the structure of PTFE that the macrophages interacted with, but in a time-dependent manner. Confocal microscopy images taken at 7, 14, and 21 days showed that the electrospun PTFE resulted in the lowest percentage of macrophage fusion, thus indicating the least possible chance of foreign body giant cell (FBGC) formation. Therefore, this study showed that electrospun PTFE with randomly oriented microfibers can provide reduced adhesion, activation, and FBGC formation of macrophages compared to the smooth and planar surface of flat PTFE and node-fibril structured surface of ePTFE. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2441-2450, 2017.

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Section: Discussionmentioning

confidence: 99%

Polytetrafluoroethylene topographies determine the adhesion, activation, and foreign body giant cell formation of macrophages

Lamichhane

Anderson

Vierhout

et al. 2017

J Biomedical Materials Res

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“…[7][8][9][10][11] Hence, various surface modification techniques have been previously developed to modify the PTFE surfaces to improve the cellular adhesion and growth. The techniques include (a) UV light irradiation, plasma irradiation, and ion irradiation treatments to incorporate amine functionality and several other oxygen containing functionalities on the PTFE surface 7,9,11,12 ; (b) bioactive coatings using proteins (fibronectin), peptides (RGD), and antibodies (CD34, CD133) 8,10,13 ; (c) biological polymer coatings using heparin. 14 Although these techniques provided promising results in improving cell adhesion and growth, all of the above-mentioned techniques involved chemical modification of PTFE surface.…”

Section: Introductionmentioning

confidence: 99%

Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene

Lamichhane

Anderson

Remund

et al. 2016

J Biomedical Materials Res

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In this study, the effect of different structures (flat, expanded, and electrospun) of polytetrafluoroethylene (PTFE) on the interactions of endothelial cells (ECs), smooth muscle cells (SMCs), and platelets was investigated. In addition, the mechanisms that govern the interactions between ECs, SMCs, and platelets with different structures of PTFE were discussed. The surface characterizations showed that the different structures of PTFE have the same surface chemistry, similar surface wettability and zeta potential, but uniquely different surface topography. The viability, proliferation, morphology, and phenotype of ECs and SMCs interacted with different structures of PTFE were investigated. Expanded PTFE (ePTFE) provided a relatively better surface for the growth of ECs. In case of SMC interactions, although all the different structures of PTFE inhibited SMC growth, a maximum inhibitory effect was observed for ePTFE. In case of platelet interactions, the electrospun PTFE provided a better surface for preventing the adhesion and activation of platelets. Thus, this study demonstrated that the responses of ECs, SMCs, and platelets strongly dependent on the surface topography of the PTFE. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2291-2304, 2016.

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“…As a result, various surface modifications have been investigated to take advantage of their high biocompatibility and inert properties [41][42][43][44][45][46]. To improve adhesion, plasma treatment [47][48][49][50][51], UV irradiation [52][53][54][55], γ-radiation [56,57], ion introduction [58][59][60][61], and polydopamine treatment [62][63][64][65][66] have been employed for surface modification; however, most of these methods require high energy to break the strong C-F bonds and add functional groups to improve adhesion.…”

Section: Introductionmentioning

confidence: 99%

Development of a Simple Spheroid Production Method Using Fluoropolymers with Reduced Chemical and Physical Damage

et al. 2021

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Establishing an in vitro–based cell culture system that can realistically simulate in vivo cell dynamics is desirable. It is thus necessary to develop a method for producing a large amount of cell aggregates (i.e., spheroids) that are uniform in size and quality. Various methods have been proposed for the preparation of spheroids; however, none of them satisfy all requirements, such as cost, size uniformity, and throughput. Herein, we successfully developed a new cell culture method by combining fluoropolymers and dot patterned extracellular matrix substrates to achieve size-controlled spheroids. First, the spheroids were spontaneously formed by culturing them two-dimensionally, after which the cells were detached with a weak liquid flow and cultured in suspension without enzyme treatment. Stable quality spheroids were easily produced, and it is expected that the introduction and running costs of the technique will be low; therefore, this method shows potential for application in the field of regenerative medicine.

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Cell growing on ion implanted polytetrafluorethylene

Cited by 14 publications

References 38 publications

Polytetrafluoroethylene topographies determine the adhesion, activation, and foreign body giant cell formation of macrophages

Polytetrafluoroethylene topographies determine the adhesion, activation, and foreign body giant cell formation of macrophages

Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene

Development of a Simple Spheroid Production Method Using Fluoropolymers with Reduced Chemical and Physical Damage

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