Cell-free therapy for canine osteoarthritis: current evidence and prospects

Sharun, Khan; Muthu, Sathish; Mankuzhy, Pratheesh; Pawde, A. M.; Chandra, Vikash; Lorenzo, José M.; Dhama, Kuldeep; Sharma, G. Taru

doi:10.1080/01652176.2022.2145620

Cited by 8 publications

(6 citation statements)

References 40 publications

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“…Ex-vivo tests have indicated that cultivated MSCs exhibit cytotoxicity to osteoarthritic synovial fluid. 8 The MSC secretome contains bioactive signals, growth factors, and extracellular matrix (ECM) molecules, with growth factors supporting chondrogenesis. Numerous studies have demonstrated the protective effects of exosomes in osteoarthritis animal models.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, exosomes have many benefits over MSCs, such as a superior safety profile with fewer side effects, a lower immunogenicity reaction, the capacity to penetrate barriers that MSCs cannot, an easier mass production and storage process at a lower cost, and fewer ethical concerns. 8,9 Exosomes are nanosized extra vesicles with 50-150 nm diameter that are secreted by MSCs and contain nucleic acids, functional proteins, and bioactive lipids. Their duties include controlling immune responses, decreasing inflammation, and healing damaged tissues.…”

Section: Introductionmentioning

confidence: 99%

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Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic and macroscopic evaluation

Powantia Pontoh,

Fiolin,

Dilogo

et al. 2024

F1000Res

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Background Current treatment of osteoarthritis (OA) mainly focused on treating symptoms. Exosome from Adipose-derived Mesenchymal Stem Cell (Ad-MSC) have been shown to delay degenerative process. This study aimed to investigate the clinical, radiological and histological impact of combined intra-articular (IA) hyaluronic acid (HA) and exosome Ad-MSCs in-vivo using a larger animal model with low-grade OA. Methods Eighteen male Ovies aries sheep underwent total lateral meniscectomy and conventional radiography was performed to confirm low-grade OA after 6 weeks. The sheep were divided into three groups, Group 1 (G1; n=6) received thrice exosome injections, G2 (n=6) received twice HA injection, and G3 (n=6) received both treatments with a 1-week interval after 10 days of meniscectomy. Clinical evaluations were conducted using the Clinical Lameness Score (CLS), radiographic with X-ray using OA score by Innes et al, while macroscopic evaluation by Osteoarthritis Research Society International (OARSI) scores. Results Lameness parameter scored lowest in G3 significantly (2.0±0.0 VS 2.7±0.52 VS 2.7±0.52; p=0.024) at the second month although the overall CLS score did not significantly differ at the 3rd month. The best improvement of conventional total OA radiographic score at the 3rd month compared to all groups (5.2±1.17 vs 6.3±0.82 vs 6.7±1.03; p=0.053). Macroscopic OARSI evaluation showed no difference (p=0.711). Conclusions Combined repeated exosome Ad-MSC and HA IA injection proven to delay OA progression, however longer duration of follow up is required to evaluate its long-term effect.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic and macroscopic evaluation

Powantia Pontoh,

Fiolin,

Dilogo

et al. 2024

F1000Res

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“…However, there are still many statements that need to be further explored and confirmed, such as the source of EVs with good therapeutic effect, the efficient way of EVs acquisition and preservation, the appropriate therapeutic dose, route and duration of administration, and the side effects and risks. 163,164 Ultrafiltration was the most used method to obtain EVs from different sources. To standardize the study of EVs, the researchers used transmission electron FIGURE 6 In the progression of Osteoarthritis (OA), MSCs-EVs promoted the formation of new blood vessels, especially H-type vessels, which provided nutritional support for the recovery of damaged cartilage.…”

Section: Preclinical and Clinical Practice Of Evsmentioning

confidence: 99%

“…As mentioned above, many in vivo and in vitro studies have gradually uncovered the mechanism of EVs in the treatment of OA or cartilage injury, such as inhibiting chondrocyte death, anti‐inflammatory, and reducing ECM degradation. However, there are still many statements that need to be further explored and confirmed, such as the source of EVs with good therapeutic effect, the efficient way of EVs acquisition and preservation, the appropriate therapeutic dose, route and duration of administration, and the side effects and risks 163,164 . Ultrafiltration was the most used method to obtain EVs from different sources.…”

Section: Preclinical and Clinical Practice Of Evsmentioning

confidence: 99%

Strategies for Cartilage Repair in Osteoarthritis Based on Diverse Mesenchymal Stem Cells‐Derived Extracellular Vesicles

Huang,

Liu,

Wang

et al. 2023

Orthopaedic Surgery

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Osteoarthritis (OA) causes disability and significant economic and social burden. Cartilage injury is one of the main pathological features of OA, and is often manifested by excessive chondrocyte death, inflammatory response, abnormal bone metabolism, imbalance of extracellular matrix (ECM) metabolism, and abnormal vascular or nerve growth. Regrettably, due to the avascular nature of cartilage, its capacity to repair is notably limited. Mesenchymal stem cells‐derived extracellular vesicles (MSCs‐EVs) play a pivotal role in intercellular communication, presenting promising potential not only as early diagnostic biomarkers in OA but also as efficacious therapeutic strategy. MSCs‐EVs were confirmed to play a therapeutic role in the pathological process of cartilage injury mentioned above. This paper comprehensively provides the functions and mechanisms of MSCs‐EVs in cartilage repair.

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“…[5][6][7] MSCs exhibit immunomodulatory effects, making them valuable in treating inflammatory and autoimmune conditions. 2,8 However, the practical application of these therapies worldwide is hindered by biosafety concerns. Quality assurance in stem cell laboratories is pivotal for ensuring the safety, consistency, and efficacy of cell-based therapies.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Bacterial Contaminants from Bone Marrow-Derived Mesenchymal Stem Cell Cultures

Banu,

Saini,

Sharun

et al. 2024

J. Pure Appl. Microbiol.

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This study aimed to assess and manage bacterial contamination in multiple batches of mesenchymal stem cell (MSC) cultures derived from rabbit bone marrow. Routine visual inspection and microscopic examination were employed for the detection of the contaminated cultures. The contaminated cultures were inoculated on Nutrient agar and multiple isolated colonies were subjected to Gram staining and biochemical characterization. Further, molecular identification of the bacterial isolates was performed using polymerase chain reaction. The determination of antibiotic susceptibility patterns was conducted using the Kirby-Bauer disc diffusion method. Among the 351 mesenchymal stem cell culture (SCC) flasks monitored, only 1.42% were found to be contaminated. Based on the phenotypic and biochemical characterization, the major bacterial contaminants were identified as Staphylococcus aureus, Bacillus spp., and Escherichia coli infiltrating during various stages of cell processing. Antibiotic susceptibility patterns revealed varying responses among isolates, crucial for effective antimicrobial strategies and maintaining aseptic conditions in SCCs. The study emphasizes the importance of regular monitoring to maintain sterile environments, validate culture quality, and uphold safety standards. The findings indicate the need to establish stringent quality control measures, crucial for the successful translation of MSC research into clinical applications. The research advocates for continuous monitoring, adherence to SOPs, and further investigations into preventive strategies for ensuring the safety and efficacy of MSC-based therapies.

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Cell-free therapy for canine osteoarthritis: current evidence and prospects

Cited by 8 publications

References 40 publications

Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic and macroscopic evaluation

Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic and macroscopic evaluation

Strategies for Cartilage Repair in Osteoarthritis Based on Diverse Mesenchymal Stem Cells‐Derived Extracellular Vesicles

Isolation and Characterization of Bacterial Contaminants from Bone Marrow-Derived Mesenchymal Stem Cell Cultures

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