Isolation and Characterization of Bacterial Contaminants from Bone Marrow-Derived Mesenchymal Stem Cell Cultures
S. Amitha Banu,
Shubham Saini,
Khan Sharun
et al.
Abstract:This study aimed to assess and manage bacterial contamination in multiple batches of mesenchymal stem cell (MSC) cultures derived from rabbit bone marrow. Routine visual inspection and microscopic examination were employed for the detection of the contaminated cultures. The contaminated cultures were inoculated on Nutrient agar and multiple isolated colonies were subjected to Gram staining and biochemical characterization. Further, molecular identification of the bacterial isolates was performed using polymera… Show more
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