Cell-free fetal DNA in the plasma of pregnant women with severe fetal growth restriction

Sekizawa, Akihiko; Jimbo, Masatoshi; Saitô, Hiroshi; Iwasaki, Mariko; Matsuoka, Ryu; Okai, Takashi; Farina, Antonio

doi:10.1067/mob.2003.27

Cited by 117 publications

(76 citation statements)

References 22 publications

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“…Overall, these data suggested that there was no aberrant concentration of our target growth markers in pregnancies with IUGR only. Indeed, previous studies involving similar number of subjects showed no significant difference in maternal plasma fetal DNA concentration for IUGR (Sekizawa et al, 2003) but higher concentrations in IUGR pregnancies with abnormal uterine artery Doppler waveforms and at risk for PET (Caramelli et al, 2003). The increased release of ADAM12 mRNA into the plasma of IUGR with PET pregnant women may be related to increased placental ADAM12 mRNA expression in preeclamptic placental tissue, which is in agreement with previous studies on ADAM12 at the protein level (Gack et al, 2005).…”

Section: Discussionsupporting

confidence: 81%

A strategy for identifying circulating placental RNA markers for fetal growth assessment

et al. 2009

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Objective To evaluate whether circulating placental mRNAs in maternal plasma could serve as markers for the assessment of fetal growth or intrauterine growth restriction (IUGR).Methods From a panel of placental transcripts detectable in maternal plasma identified by microarray previously, we chose growth-related transcripts, namely CSH1, GH2, KISS1, and ADAM12, as potential growth markers. Relationships between the maternal plasma mRNA concentrations with several fetal growth indicators were studied. Maternal plasma mRNA concentrations from IUGR pregnancies with or without pre-eclampsia (PET) were compared with gestational age matched controls cross-sectionally and longitudinally. The four transcripts were quantified by one-step real-time RT-PCR.Results Maternal plasma GH2 mRNA significantly correlated with birth weight and fetal biometric measurements. Maternal plasma ADAM12 mRNA concentration was significantly higher in IUGR with PET than normal pregnancies in the cross-sectional comparison. No significant difference was observed for all markers between IUGR without PET and controls in both the cross-sectional and longitudinal comparisons. ConclusionThis study presents a potential strategy in identifying surrogate markers for the study of fetal growth. Circulating GH2 mRNA in maternal plasma appeared to be associated with fetal growth. The utility of this strategy and the currently assessed markers could be explored in further studies.

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Section: Discussionsupporting

confidence: 81%

A strategy for identifying circulating placental RNA markers for fetal growth assessment

et al. 2009

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“…Interestingly, amniotic fluid concentration of S100B was found to sensitively and specifically predict spontaneous intrauterine fetal death (Florio et al 2004). Although an early study conducted by Sekisawa et al found no significant difference in maternal plasma levels of cffDNA in nine women with IUGR compared with 20 gestation age-matched controls (Sekizawa et al 2003), more recent studies using larger cohorts found significant increases in women with IUGR (Smid et al 2006, Al Nakib et al 2009, Alberry et al 2009. Similarly, circulating mitochondrial DNA was also found to be increased in patients with IUGR (Colleoni et al 2010).…”

Section: Intrauterine Growth Restrictionmentioning

confidence: 99%

Sterile inflammation and pregnancy complications: a review

Nadeau-Vallée¹,

Obari²,

Palacios³

et al. 2016

Reproduction

215

161

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Inflammation is essential for successful embryo implantation, pregnancy maintenance and delivery. In the last decade, important advances have been made in regard to endogenous, and therefore non-infectious, initiators of inflammation, which can act through the same receptors as pathogens. These molecules are referred to as damage-associated molecular patterns (DAMPs), and their involvement in reproduction has only recently been unraveled. Even though inflammation is necessary for successful reproduction, untimely activation of inflammatory processes can have devastating effect on pregnancy outcomes. Many DAMPs, such as uric acid, high-mobility group box 1 (HMGB1), interleukin (IL)-1 and cell-free fetal DNA, have been associated with pregnancy complications, such as miscarriages, preeclampsia and preterm birth in preclinical models and in humans. However, the specific contribution of alarmins to these conditions is still under debate, as currently there is lack of information on their mechanism of action. In this review, we discuss the role of sterile inflammation in reproduction, including early implantation and pregnancy complications. Particularly, we focus on major alarmins vastly implicated in numerous sterile inflammatory processes, such as uric acid, HMGB1, IL-1α and cell-free DNA (especially that of fetal origin) while giving an overview of the potential role of other candidate alarmins.Reproduction (2016) 152 R277-R292

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“…The levels of total extracellular DNA, measured by quantifying the ubiquitous b-globin (GLO) and/or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) sequences in maternal circulation, were also found to be increased in this pathology (early/intermediate/late PEP, IUGR before and after 34 weeks) by several independent research groups (Zhong et al, 2001;Sekizawa et al, 2003) (Table 1).…”

Section: Extracellular Dna In Maternal Circulationmentioning

confidence: 99%

“…Caramelli et al (2003) demonstrated only a small increase in fetal DNA concentrations, determined using SRY TagMan PCR assays, in pregnancies with abnormal uterine artery Doppler waveforms that developed IUGR. Sekizawa et al (2003) demonstrated that fetal DNA concentrations were similar in both IUGR and normal subjects, determined using DYS-14 sequence quantification in maternal plasma.…”

Section: Extracellular Dna In Maternal Circulationmentioning

confidence: 99%

“…These data were later confirmed by other investigators using real-time quantitative PCR and either the SRY gene or the DYS-14 sequence as markers to differentiate between normal and complicated pregnancies (Smid et al, 2001;Zhong et al, 2001;Lau et al, 2002;Farina et al, 2004b;Engel et al, 2007;Alberry et al, 2009;Hromadnikova et al, 2009Hromadnikova et al, , 2010b. It was suggested that a rise in fetal DNA represented a valuable marker of placenta-related pregnancy complications, which could predict PE several weeks before clinical manifestation; however, with regard to IUGR, a rise in fetal DNA was less well correlated (Caramelli et al, 2003;Sekizawa et al, 2003;Farina et al, 2004c;Zhong et al, 2007;Hromadnikova et al, 2010b). Caramelli et al (2003) demonstrated only a small increase in fetal DNA concentrations, determined using SRY TagMan PCR assays, in pregnancies with abnormal uterine artery Doppler waveforms that developed IUGR.…”

Section: Extracellular Dna In Maternal Circulationmentioning

confidence: 99%

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Extracellular Nucleic Acids in Maternal Circulation as Potential Biomarkers for Placental Insufficiency

Hromadníková

2012

DNA and Cell Biology

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Cell-free fetal DNA in the plasma of pregnant women with severe fetal growth restriction

Cited by 117 publications

References 22 publications

A strategy for identifying circulating placental RNA markers for fetal growth assessment

A strategy for identifying circulating placental RNA markers for fetal growth assessment

Sterile inflammation and pregnancy complications: a review

Extracellular Nucleic Acids in Maternal Circulation as Potential Biomarkers for Placental Insufficiency

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