Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells

Ikeda, Katsuo; Nagata, Shogo; Okitsu, Teru; Takeuchi, Shoji

doi:10.1038/s41598-017-03246-2

Cited by 32 publications

(27 citation statements)

References 32 publications

(44 reference statements)

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“…In this study, the C2C12 cells were contained in the core-shell hydrogel fiber, which is suitable for three-dimensional tissue reconstruction [21]. Moreover, the hydrogel fiber structure could induce exchange of O 2 , CO 2 , and nutrients [32]. Therefore, our skeletal muscle reconstruction system using the cell-laden hydrogel fiber and mechanical stretching stimuli is anticipated to be applicable for in vitro tissue regeneration and clinical applications.…”

Section: Resultsmentioning

confidence: 99%

Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers

et al. 2019

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Engineering of the skeletal muscles has attracted attention for the restoration of damaged muscles from myopathy, injury, and extraction of malignant tumors. Reconstructing a three-dimensional muscle using living cells could be a promising approach. However, the regenerated tissue exhibits a weak construction force due to the insufficient tissue maturation. The purpose of this study is to establish the reconstruction system for the skeletal muscle. We used a cell-laden core-shell hydrogel microfiber as a three-dimensional culture to control the cellular orientation. Moreover, to mature the muscle tissue in the microfiber, we also developed a custom-made culture device for imposing cyclic stretch stimulation using a motorized stage and the fiber-grab system. As a result, the directions of the myotubes were oriented and the mature myotubes could be formed by cyclic stretch stimulation.

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Section: Resultsmentioning

confidence: 99%

Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers

et al. 2019

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“…So far, microfluidics‐based microfibers have been mainly fabricated using sodium alginate (NaA) because of its biodegradable and biocompatible properties and its rapid gelation process in the presence of divalent cations (e.g., calcium ion) . However, Ca‐alginate (CaA) might disintegrate in the physiological environment due to the loss of calcium ions .…”

Section: Introductionmentioning

confidence: 99%

Simple fabrication of inner chitosan‐coated alginate hollow microfiber with higher stability

Liu

Wang

et al. 2019

J Biomed Mater Res

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Sodium alginate (NaA) has been widely used as microfiber‐based scaffold material. However, Ca‐alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long‐term application in tissue engineering. In this work, to enhance the stability of Ca‐alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca‐alginate hollow microfiber by one step via a microfluidic device. A more stable composite microfiber with double cross‐linking layers was generated. The stability of the microfiber was studied in the PBS solution (pH 7.4) to identify the coating effect on the hollow structure. The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca‐alginate shell was disintegrated by PBS solution. In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber. HepG2 cells in the microfibers displayed favorable cell viability and proliferation ability. We believe that this work will lead to the development of innovative methodologies and materials for both cell culture and tissue engineering application. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2527–2536, 2019.

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“…Additionally, rod‐shaped constructs of >4 mm in length could be fabricated. It has been difficult to obtain large constructs (>2 mm in diameter) using ordinary methods . The technology presented in this study allowed us to fabricate large constructs; therefore, this approach should readily allow the bundling of rod‐shaped constructs composed of various cell types for the fabrication of complex tissues.…”

Section: Discussionmentioning

confidence: 99%

“…It has been difficult to obtain large constructs (>2 mm in diameter) using ordinary methods. 32 The technology presented in this study allowed us to fabricate large constructs; therefore, this approach should readily allow the bundling of rod-shaped constructs composed of various cell types for the fabrication of complex tissues. In addition, it should be possible to combine this technology with a cell sheet technique for the development of tissue engineering science.…”

Section: Discussionmentioning

confidence: 99%

Fabricating large‐scale three‐dimensional constructs with living cells by processing with syringe needles

Sasaki

Katata

Abe

et al. 2019

J Biomedical Materials Res

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Three‐dimensional (3D) cell constructs composed only of cells and cell‐secreted extracellular matrix have been attractive biomaterials for tissue engineering technology; however, controlling construct morphology and eliminating dead cells after fabrication remain a challenge. It has been hypothesized that moderate stress could shape constructs and eliminate dead cells. The purpose of this study was to establish an easily available technology for shaping 3D cell constructs and eliminating dead cells postfabrication. To achieve these objectives, spherical cell constructs composed of L‐929 fibroblasts were processed using different sized syringe needles. Our results revealed that large‐scale rod‐shaped cell constructs could be fabricated, and that their diameters could be controlled according to the size of the syringe needle. Additionally, cell viability assays showed that >94% of cells in the rod‐shaped constructs were viable, suggesting that dead cells, which have low adhesion force, were dispersed when compressive stress was applied during passage through the needle. The technology described in this study will be promising for future tissue engineering, especially for fabricating elongated tissues such as nerves and blood vessels. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 904–909, 2019.

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Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells

Cited by 32 publications

References 32 publications

Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers

Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers

Simple fabrication of inner chitosan‐coated alginate hollow microfiber with higher stability

Fabricating large‐scale three‐dimensional constructs with living cells by processing with syringe needles

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