Fabricating large‐scale three‐dimensional constructs with living cells by processing with syringe needles

Sasaki, Jun; Katata, Chihiro; Abe, Gabriela L.; Matsumoto, Takuya; Imazato, Satoshi

doi:10.1002/jbm.a.36613

Cited by 3 publications

(4 citation statements)

References 31 publications

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“…The findings suggested that TEP might regenerate bone tissue directly via the surviving cells attached to it. This suggested that any size and shape of bone defect could be successfully repaired using this simulated periosteal approach without pre-vascularization of constructs, which has been a critical challenge in the area of tissue engineering until now [26,27].…”

Section: Discussionmentioning

confidence: 99%

“…Then, the remaining submucosal layer was treated with a series of chemical decellularization steps, given detergent treatment, lyophilized, and sterilized [19]. Finally, all the samples were freeze-dried at -70°C in a lyophilizer, sealed into hermetic packages, and then sterilized using Co-60 gamma irradiation (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35).…”

Section: Sis Preparation and Tep Fabricationmentioning

confidence: 99%

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Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model

Zhao

et al. 2020

Tissue Eng Regen Med

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Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair using TEP. Methods: We performed a subtotal resection of the scapula in 36 rabbits to establish a large irregular bone defect model. The rabbits were then randomly divided into three groups (n = 12 per group) and the defects were treated with TEP (Group 1), allogeneic deproteinized bone (DPB) (Group 2) or a hybrid of TEP and DPB (Group 3). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed to detect bone healing. Ink-formaldehyde perfusion was introduced to qualitatively analyze vascularization in TEP engineered new bone. Results: The repair of scapular defects was diverse in all groups, shown by radiographic and histological tests. The radiographic scores in Group 1 and Group 3 were significantly higher than Group 2 at 8 and 12 weeks (p < 0.05). Histological scores further proved that Group 1 had significantly greater new bone formation compared to Group 3 (p < 0.05), while Group 2 had the lowest osteogenesis at all time-points (p < 0.001). Ink-formaldehyde perfusion revealed aboundant microvessels in TEP engineered new bone. Conclusion: We conclude that TEP is promising for the repair of large irregular bone defects. As a 3D scaffold, DPB could provide mechanical support and a shaping guide when combined with TEP. TEP engineered new bone has aboundant microvessels.

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Section: Discussionmentioning

confidence: 99%

Section: Sis Preparation and Tep Fabricationmentioning

confidence: 99%

Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model

Zhao

et al. 2020

Tissue Eng Regen Med

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“…Moreover, it produces low immune reaction [24].The thickness (100 ± 20 µm) of SIS is lower than the critical value (500 µm), can permit attached cells to survive on diffusion of nutrients from the interstitial fluid in early stage after implanting [25]. Successful bone formation was observed in Group1 and 3 via this TEP approach in this study.The findings suggested that TEP might regenerate bone tissue directly via survived cells attached on it.This hints that any size and any shape of bone defect can successfully repair via mimic peristeal approach without pre-vascularization of constructs, which is a critical challenge in tissure engineering area till now [26][27].…”

Section: Discussionmentioning

confidence: 99%

Irregular bone defect repair via tissue-engineered periosteum approach in rabbit model.

Zhao

Yu²,

Zhang³

et al. 2019

Preprint

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Background As an alternative of bone grafts for defect repair, tissue engineering is much promising for clinical application. In previous studies, we have succeeded in repair of long bone defect with homemade tissue-engineered periosteum (TEP), of which is fabraicated by incorporating osteogenically induced mesenchymal stem cells (MSCs) of rabbits with a scaffold of small intestinal submucosa (SIS).Methods In this study, we are aimed to discuss the feasibility of allogenic irregular bone defect repair with the TEP. Thirty-six rabbits whose scapulas were subtotally resected to establish large irregular bone defects model in allogenic rabbits. The defects were treat respectively with TEP (Group 1, n=12), allogenic deproteinized bone (DPB) (Group 2, n=12) and hybrid of TEP and DPB (Group 3, n=12). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed.ResultsThe findings suggested that the radiographic score in TEP-DPB hybrided implantation (Group 3) was higher than TEP or DPB grafting only (p<0.05).But that was inconsistent with histological findings, which Group1 appeared to possess significantly higher bone formation than Group 2 (p<0.05) and Group3 has higher new bone volume than that of Group 2 (p<0.05).Conclusion We conclude that TEP is a promising alternative in repair of large irregular bone defect.DPB served as a 3D scaffold in combining TEP could provide mechanical support and shaping guide, but hinder new bone formation via TEP approach due to retard degradation.

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“…The technology for fabricating 3D cell constructs can be applied to other types of cells as well as the BMSCs and DPSCs described in this review [ 98 , 99 ]. Previously, we reported that 3D cell constructs were fabricated from the fibroblast-like L929 cells [ 98 ]. This study demonstrated a method for eliminating the dead cells from the cell construct using a syringe needle.…”

Section: Further Application Of 3d Cell Constructsmentioning

confidence: 99%

Large three-dimensional cell constructs for tissue engineering

Sasaki

Abe

et al. 2021

Science and Technology of Advanced Materials

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Much research has been conducted on fabricating biomimetic biomaterials in vitro. Tissue engineering approaches are often conducted by combining cells, scaffolds, and growth factors. However, the degradation rate of scaffolds is difficult to control and the degradation byproducts occasionally limit tissue regeneration. To overcome these issues, we have developed a novel system using a thermo-responsive hydrogel that forms scaffold-free, three-dimensional (3D) cell constructs with arbitrary size and morphology. 3D cell constructs prepared using bone marrow-derived stromal stem cells (BMSCs) exhibited self-organizing ability and formed bone-like tissue with endochondral ossification. Endothelial cells were then introduced into the BMSC construct and a vessel-like structure was formed within the constructs. Additionally, the bone formation ability was promoted by endothelial cells and cell constructs could be freeze-dried to improve their clinical application. A pre-treatment with specific protein protectant allowed for the fabrication of novel bone substitutes composed only of cells. This 3D cell construct technology using thermo-responsive hydrogels was then applied to other cell species. Cell constructs composed of dental pulp stem cells were fabricated, and the resulting construct regenerated pulp-like tissue within a human pulpless tooth. In this review, we demonstrate the approaches for the in vitro fabrication of bone and dental pulp-like tissue using thermo-responsive hydrogels and their potential applications.

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Fabricating large‐scale three‐dimensional constructs with living cells by processing with syringe needles

Cited by 3 publications

References 31 publications

Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model

Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model

Irregular bone defect repair via tissue-engineered periosteum approach in rabbit model.

Large three-dimensional cell constructs for tissue engineering

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