Cell-Electrospinning and Its Application for Tissue Engineering

Hong, Jisu; Yeo, Miji; Yang, Gi Hoon; Kim, GeunHyung

doi:10.3390/ijms20246208

Cited by 134 publications

(139 citation statements)

References 65 publications

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“…The 2D structures are then used as a scaffold, and can be coated with collagen for more physiological properties of basement membrane. More recently, electrospinning has been used to directly embed cells into the biopolymers fibers (Hong et al 2019) and provides promising applications in the field of tissue engineering when coupled with hydrogels solutions and 3D printing.…”

Section: Electrospun Membranesmentioning

confidence: 99%

Characterization of immune cell migration using microfabrication

Vesperini

Montalvo

et al. 2021

Biophys Rev

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The immune system provides our defense against pathogens and aberrant cells, including tumorigenic and infected cells. Motility is one of the fundamental characteristics that enable immune cells to find invading pathogens, control tissue damage, and eliminate primary developing tumors, even in the absence of external treatments. These processes are termed “immune surveillance.” Migration disorders of immune cells are related to autoimmune diseases, chronic inflammation, and tumor evasion. It is therefore essential to characterize immune cell motility in different physiologically and pathologically relevant scenarios to understand the regulatory mechanisms of functionality of immune responses. This review is focused on immune cell migration, to define the underlying mechanisms and the corresponding investigative approaches. We highlight the challenges that immune cells encounter in vivo, and the microfabrication methods to mimic particular aspects of their microenvironment. We discuss the advantages and disadvantages of the proposed tools, and provide information on how to access them. Furthermore, we summarize the directional cues that regulate individual immune cell migration, and discuss the behavior of immune cells in a complex environment composed of multiple directional cues.

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Section: Electrospun Membranesmentioning

confidence: 99%

Characterization of immune cell migration using microfabrication

Vesperini

Montalvo

et al. 2021

Biophys Rev

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“…Further advances in tissue engineering and regenerative medicine are expected from the convergence of cell electrospinning (i.e., using a coaxial bio-electrospray) with 3D bioprinting. The former allows the controlled distribution of cells, encapsulated in nanosized fibers (reviewed in [ 92 ]). In addition, commonly bioprinted constructs are not ready for in vivo applications and have to follow a maturation process, where architectural changes and remodeling are recognized as the fourth dimension of bioprinting [ 93 ].…”

Section: Limitations and Future Directionsmentioning

confidence: 99%

Overview of Current Advances in Extrusion Bioprinting for Skin Applications

Perez‐Valle

Amo

Andı́a

2020

IJMS

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Bioprinting technologies, which have the ability to combine various human cell phenotypes, signaling proteins, extracellular matrix components, and other scaffold-like biomaterials, are currently being exploited for the fabrication of human skin in regenerative medicine. We performed a systematic review to appraise the latest advances in 3D bioprinting for skin applications, describing the main cell phenotypes, signaling proteins, and bioinks used in extrusion platforms. To understand the current limitations of this technology for skin bioprinting, we briefly address the relevant aspects of skin biology. This field is in the early stage of development, and reported research on extrusion bioprinting for skin applications has shown moderate progress. We have identified two major trends. First, the biomimetic approach uses cell-laden natural polymers, including fibrinogen, decellularized extracellular matrix, and collagen. Second, the material engineering line of research, which is focused on the optimization of printable biomaterials that expedite the manufacturing process, mainly involves chemically functionalized polymers and reinforcement strategies through molecular blending and postprinting interventions, i.e., ionic, covalent, or light entanglement, to enhance the mechanical properties of the construct and facilitate layer-by-layer deposition. Skin constructs manufactured using the biomimetic approach have reached a higher level of complexity in biological terms, including up to five different cell phenotypes and mirroring the epidermis, dermis and hypodermis. The confluence of the two perspectives, representing interdisciplinary inputs, is required for further advancement toward the future translation of extrusion bioprinting and to meet the urgent clinical demand for skin equivalents.

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“…This method could lead to non‐uniform distribution of cells over a given scaffold architecture, resulting in regions of acellularity 80 . An alternative approach is represented by cell electrospinning, which generates fibrous structures with living cells embedded within 81 . For instance, myoblast cell‐laden constructs with highly aligned microstructure and high cell viability were fabricated for skeletal muscle regeneration 82 .…”

Section: Electrospinningmentioning

confidence: 99%

Electrospinning and 3D bioprinting for intervertebral disc tissue engineering

et al. 2020

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Intervertebral disc (IVD) degeneration is a major cause of low back pain and represents a massive socioeconomic burden. Current conservative and surgical treatments fail to restore native tissue architecture and functionality. Tissue engineering strategies, especially those based on 3D bioprinting and electrospinning, have emerged as possible alternatives by producing cell-seeded scaffolds that replicate the structure of the IVD extracellular matrix. In this review, we provide an overview of recent advancements and limitations of 3D bioprinting and electrospinning for the treatment of IVD degeneration, focusing on future areas of research that may contribute to their clinical translation.

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Cell-Electrospinning and Its Application for Tissue Engineering

Cited by 134 publications

References 65 publications

Characterization of immune cell migration using microfabrication

Characterization of immune cell migration using microfabrication

Overview of Current Advances in Extrusion Bioprinting for Skin Applications

Electrospinning and 3D bioprinting for intervertebral disc tissue engineering

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