Cell damage evaluation of mammalian cells in cell manipulation by amplified femtosecond ytterbium laser

Hong, Zhen Yi; Iino, Takanori; Hagihara, Hiroaki; Maeno, Takanori; Okano, Kazunori; Yasukuni, Ryohei; Hosokawa, Yoichiroh

doi:10.1007/s00339-018-1677-3

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…Bio-samples retain their viability when manipulated by a laser, as evidenced by previous studies 38 , 39 . Because of the different cell states and sizes among individual PC-12 cells, the fluorescence intensity of each cell should also be different, as shown in Fig.…”

Section: Resultssupporting

confidence: 68%

“…A pocket structure is put on the channel wall next to the fluorescence detection region to ensure that all impulsive forces travel unidirectionally and focus at the sorting region 34 and to prevent the potential negative influence induced by the laser irradiation on the target. The pocket structure for laser irradiation is integrated into the microchannel to increase the distance (it should be > 10 μm 35 ) between the focus point and the target. In addition, the strength and duration of the impulsive force are evaluated as a function of the number of femtosecond pulses by analyzing the streamline profile of the polystyrene beads.…”

Section: Introductionmentioning

confidence: 99%

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Microsecond cell triple-sorting enabled by multiple pulse irradiation of femtosecond laser

Kiya

Tang

Tanaka

et al. 2023

Sci Rep

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Femtosecond-laser-assisted cell manipulation, as one of the high throughput cell sorting techniques, is tailored for single-step multiple sorting based on controllable impulsive force. In this paper, femtosecond laser pulses are focused within a pocket structure and they induce an impulse force acting on the flowing objects. The impulsive force is shown to be controllable by a new method to adjust the femtosecond pulse properties. This allows precise streamline manipulation of objects having various physical qualities (e.g., weight and volume). The pulse energy, pulse number, and pulse interval of the femtosecond laser are altered to determine the impulsive force strength. The method is validated in single cell or bead triple-sorting experiments and its capability to perform streamline manipulation in as little as 10 μs is shown. The shift profiles of the beads acting under the impulsive force are studied in order to better understand the sorting mechanism. Additionally, beads and cells with different fluorescence intensities are successfully detected and directed into different microchannels, with maximum success rates of 90% and 64.5%, respectively. To sum up, all results suggest that this method has the potential to sort arbitrary subpopulations by altering the number of femtosecond pulses and that it takes the first step toward developing a single-step multi-selective system.

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Section: Resultssupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%