Cell-cycle regulation of yeast histone mRNA

Hereford, Lynna M.; Osley, Mary Ann; Ludwig, J R; McLaughlin, Calvin S.

doi:10.1016/0092-8674(81)90326-3

Cited by 326 publications

(216 citation statements)

References 35 publications

Supporting

Mentioning

195

Contrasting

Unclassified

Order By: Relevance

“…Additionally, histone levels are unaffected by GAL-CLB2. The sharpness of the peak in histone regulation is worth noting, both because it gives a good impression of the degree of synchronization and because the histones were the first genes for which periodic regulation was discovered (Hereford et al, 1981).…”

Section: The S and M Clustersmentioning

confidence: 99%

See 1 more Smart Citation

Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

Spellman

Sherlock

Zhang

et al. 1998

MBoC

4,305

118

4,411

View full text Add to dashboard Cite

We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures synchronized by three independent methods: ␣ factor arrest, elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using periodicity and correlation algorithms, we identified 800 genes that meet an objective minimum criterion for cell cycle regulation. In separate experiments, designed to examine the effects of inducing either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins. Furthermore, we analyzed our set of cell cycle-regulated genes for known and new promoter elements and show that several known elements (or variations thereof) contain information predictive of cell cycle regulation. A full description and complete data sets are available at

show abstract

Section: The S and M Clustersmentioning

confidence: 99%

“…In 1981 Hereford and coworkers discovered that yeast histone mRNAs oscillate in abundance during the cell division cycle (Hereford et al, 1981). To date 104 messages that are cell cycle regulated have been identified using traditional methods, and it was estimated that some 250 cell cycle-regulated genes might exist (Price et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

Spellman

Sherlock

Zhang

et al. 1998

MBoC

4,305

118

4,411

View full text Add to dashboard Cite

show abstract

“…There have been also a number of reports on gene expression considered in terms of variations in mRNAs during the cell cycle in mammalian cells and yeast. Several genes related to DNA synthesis, such as those that encoded dihydrofolate reductase (20), thymidine kinase (38), thymidylate synthetase (6), and histones (22,34), are associated with transient increases in levels of their respective mRNAs during the cell cycle. It has been suggested that proto-oncogenes are involved in the intracellular events that govern cellular proliferation in mammalian cells (19).…”

mentioning

confidence: 99%

Phase-Specific Polypeptides and Poly(A)⁺ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells

Kodama

Kawakami²,

Watanabe³

et al. 1989

Plant Physiol.

View full text Add to dashboard Cite

This study shows an overall analysis of gene expression during the cell cycle in synchronous suspension cultures of Catharanthus roseus cells. First, the cellular cytoplasmic proteins were fractionated by two-dimensional gel electrophoresis and visualized by staining with silver. Seventeen polypeptides showed qualitative or quantitafive changes during the cell cycle. Second, the rates of synthesis of cytoplasmic proteins were also investigated by autoradiography by labeling cells with [S]methionine at each phase of the cell cycle. The rates of synthesis of 13 polypeptides were found to vary during the cell cycle. The silverstained electrophoretic pattem of proteins in the G2 phase in particular showed characteristic changes in levels of polypeptides, while the rates of synthesis of polypeptides synthesized during the G2 phase did not show such phase-specific changes.This result suggests that posttranslational processing of polypeptides occurs during or prior to the G2 phase. In the G1 and S phases and during cytokinesis, several other polypeptides were specifically synthesized. Finally, the variation of mRNAs was analyzed from the autoradiograms of In vitro translafton products of poly(A)+ RNA isolated at each phase. Three poly(A)+ RNAs increased in amount from the GI to the S phase and one poly (A)+ RNA increased preferentially from the G2 phase to cytokinesis.The cell cycle in eukaryotes consists of an ordered sequence of events, which include DNA synthesis, replication of organelles, and mitosis, and which are controlled by the ordered expression of genes. Many studies have been undertaken to examine the changes in protein patterns through which the sequential action of genes during the cell cycle is reflected, and to demonstrate that genes involved in cell proliferation are expressed transiently during the cell cycle. Two-dimensional gel electrophoresis has revealed the presence of several phase-specific proteins in mammalian cells which appear in the G2 phase (1) or which are synthesized in the S phase (10) and the G2/M phase (41). There are also reports (8,15,29) that no unique proteins are synthesized during the cell cycle in HeLa cells and yeast.

show abstract

“…Hereford et al, [12,13] had used an unassigned transcript of about 1000 bases, which hybridized to the same DNA fragment as the histone H2A/H2B messengers, as a constitutively expressed internal standard in their experiments to demonstrate cell cycle control of histone gene transcription. It is clear now that this RNA species is the messenger of AKY2, formerly termed protein 1 [13].…”

Section: Discussionmentioning

confidence: 99%

“…Their transcription is correlated with the progression of the cell cycle and turned on for only a short period of time at the beginning of the S-phase [12][13][14][15]. Here we report that the gene for the mitochondrial isozyme of adenylate kinase (AKY2) is arranged in tandem 3' of the HTA1 gene at the rather short distance of 560 bp.…”

Section: Frgmentioning

confidence: 99%

Yeast adenylate kinase is transcribed constitutively from a promoter in the short intergenic region to the histone H2A‐1 gene

et al. 1988

View full text Add to dashboard Cite

Yeast mitochondrial adenylate kinase (high molecular mass form, gene locus: AKY2) is encoded on chromosome IV of the same DNA strand as histone H2A-I. The nontranslated intergenic region spans 560 bp, the nontranscribed spacer can be estimated to comprise at most 300 bp. The TATA-box sequence is contained in a striking environment consisting of 20 alternating pyrimidines and purines. The AKY2 transcript is made constitutively: (i) the cellular mRNA concentration does not vary significantly with either growth conditions or elapse of the cell cycle; (ii) p-galactosidase activity is about constant in yeast cells grown on various carbon sources after transformation with AKY2-promoter/lacZ fusions; (iii) primer elongation analysis shows that utilization of 5 initiation sites is qualitatively and quantitatively independent of the growth conditions and the carbon source used; (iv) Western blot analysis and adenylate kinase activity measurements indicate the absence of post-transciptional controls as well.Mitochondrial adenylate kinase; Constitutive promoter; Histone H2AI intergenic region; Promoter/lacZ fusion; Nucleotide sequence;(Saccharomyces cerevisiae)

show abstract

Cell-cycle regulation of yeast histone mRNA

Cited by 326 publications

References 35 publications

Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

Phase-Specific Polypeptides and Poly(A)⁺ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells

Yeast adenylate kinase is transcribed constitutively from a promoter in the short intergenic region to the histone H2A‐1 gene

Contact Info

Product

Resources

About

Cell-cycle regulation of yeast histone mRNA

Cited by 326 publications

References 35 publications

Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

Phase-Specific Polypeptides and Poly(A)+ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells

Yeast adenylate kinase is transcribed constitutively from a promoter in the short intergenic region to the histone H2A‐1 gene

Contact Info

Product

Resources

About

Phase-Specific Polypeptides and Poly(A)⁺ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells