Cell cycle regulation of embryonic stem cells and mouse embryonic fibroblasts lacking functional Pax7

Czerwińska, Areta M.; Nowacka, Joanna; Aszer, Magdalena; Gawrzak, Sylwia; Archacka, Karolina; Fogtman, Anna; Iwanicka-Nowicka, Roksana; Jańczyk-Ilach, Katarzyna; Koblowska, Marta; Ciemerych, Maria A.; Grabowska, Iwona

doi:10.1080/15384101.2016.1231260

Cited by 10 publications

(22 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Next, the absence of functional Pax7 did not influence Nfix transcription level and fetal myoblast formation during in vitro differentiation of ESCs [3]. The major differences between Pax7+/+ and Pax7−/− cells concerned the cell cycle regulation and miRNA expression [3,4]. Thus, our in vitro studies confirmed that the absence of functional Pax7 caused positive effect on ESC proliferation and early stages of their myogenic differentiation, as well as on the proliferation of mouse embryonic fibroblasts [4].…”

Section: Introductionsupporting

confidence: 59%

“…In vitro cultured mouse ESCs are unable to differentiate spontaneously into fully formed, i.e., innervated and vascularized, skeletal muscle tissue. The most common in vitro approach to induce myogenic differentiation of ESCs depends either on the formation of embryoid bodies (EBs) and EB outgrowths [2][3][4][5][6][7] or ESC incubation in the presence of demethylating agents [3,8]. Unfortunately, these methods are rather inefficient and poorly controllable.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pax7 as molecular switch regulating early and advanced stages of myogenic mouse ESC differentiation in teratomas

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background: Pluripotent stem cells present the ability to self-renew and undergo differentiation into any cell type building an organism. Importantly, a lot of evidence on embryonic stem cell (ESC) differentiation comes from in vitro studies. However, ESCs cultured in vitro do not necessarily behave as cells differentiating in vivo. For this reason, we used teratomas to study early and advanced stages of in vivo ESC myogenic differentiation and the role of Pax7 in this process. Pax7 transcription factor plays a crucial role in the formation and differentiation of skeletal muscle precursor cells during embryonic development. It controls the expression of other myogenic regulators and also acts as an anti-apoptotic factor. It is also involved in the formation and maintenance of satellite cell population. Methods: In vivo approach we used involved generation and analysis of pluripotent stem cell-derived teratomas. Such model allows to analyze early and also terminal stages of tissue differentiation, for example, terminal stages of myogenesis, including the formation of innervated and vascularized mature myofibers. Results: We determined how the lack of Pax7 function affects the generation of different myofiber types. In Pax7 −/− teratomas, the skeletal muscle tissue occupied significantly smaller area, as compared to Pax7+/+ ones. The proportion of myofibers expressing Myh3 and Myh2b did not differ between Pax7+/+ and Pax7−/− teratomas. However, the area of Myh7 and Myh2a myofibers was significantly lower in Pax7−/− ones. Molecular characteristic of skeletal muscles revealed that the levels of mRNAs coding Myh isoforms were significantly lower in Pax7−/− teratomas. The level of mRNAs encoding Pax3 was significantly higher, while the expression of Nfix, Eno3, Mck, Mef2a, and Itga7 was significantly lower in Pax7−/− teratomas, as compared to Pax7+/+ ones. We proved that the number of satellite cells in Pax7−/− teratomas was significantly reduced. Finally, analysis of neuromuscular junction localization in samples prepared with the iDISCO method confirmed that the organization of neuromuscular junctions in Pax7−/− teratomas was impaired.

show abstract

Section: Introductionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Pax7 as molecular switch regulating early and advanced stages of myogenic mouse ESC differentiation in teratomas

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Affymetrix technology ( ) and Partek Genomics Suite Software ( ) are well world-wide established methods and are routinely used in Corelab ( ) [ 54 , 55 , 56 ], where microarray analysis was performed.…”

Section: Methodsmentioning

confidence: 99%

Time-Course Microarray Analysis Reveals Differences between Transcriptional Changes in Tomato Leaves Triggered by Mild and Severe Variants of Potato Spindle Tuber Viroid

Więsyk

Iwanicka-Nowicka

Fogtman

et al. 2018

Viruses

Self Cite

View full text Add to dashboard Cite

Viroids are small non-capsidated non-coding RNA replicons that utilize host factors for efficient propagation and spread through the entire plant. They can incite specific disease symptoms in susceptible plants. To better understand viroid-plant interactions, we employed microarray analysis to observe the changes of gene expression in “Rutgers” tomato leaves in response to the mild (M) and severe (S23) variants of potato spindle tuber viroid (PSTVd). The changes were analyzed over a time course of viroid infection development: (i) the pre-symptomatic stage; (ii) early symptoms; (iii) full spectrum of symptoms and (iv) the so-called ‘recovery’ stage, when stem regrowth was observed in severely affected plants. Gene expression profiles differed depending on stage of infection and variant. In S23-infected plants, the expression of over 3000 genes was affected, while M-infected plants showed 3-fold fewer differentially expressed genes, only 20% of which were specific to the M variant. The differentially expressed genes included many genes related to stress; defense; hormone metabolism and signaling; photosynthesis and chloroplasts; cell wall; RNA regulation, processing and binding; protein metabolism and modification and others. The expression levels of several genes were confirmed by nCounter analysis.

show abstract

“…2 In this issue of the Cell Cycle the same group of Dr. Iwona Grabowska presents further data on the involvement of Pax7 in cell cycle regulation of ESCs and, surprisingly, also mouse embryonic fibroblasts (MEFs). 3 In normal cells Pax7 was so far linked specifically to SCs, although one study demonstrated its expression in spermatogonia in adult testes. 4 and some others in pituitary melanotropes of the intermediate lobe.…”

mentioning

confidence: 99%

“…Quantitative comparison of Pax7 expression in MEFs with the level observed in differentiating ESCs would be informative. As Czerwinska et al have studied 3 populations of MEFs: asynchronously dividing, serum starved and proliferating after synchronisation by serum starvation, 3 determination whether Pax7 expression may vary in the wild type (WT) cells at different stages would be also of interest.…”

mentioning

confidence: 99%

Many roles for Pax7

Dulak

2016

Cell Cycle

View full text Add to dashboard Cite

Cell cycle regulation of embryonic stem cells and mouse embryonic fibroblasts lacking functional Pax7

Cited by 10 publications

References 60 publications

Pax7 as molecular switch regulating early and advanced stages of myogenic mouse ESC differentiation in teratomas

Pax7 as molecular switch regulating early and advanced stages of myogenic mouse ESC differentiation in teratomas

Time-Course Microarray Analysis Reveals Differences between Transcriptional Changes in Tomato Leaves Triggered by Mild and Severe Variants of Potato Spindle Tuber Viroid

Many roles for Pax7

Contact Info

Product

Resources

About