Cell cycle progression and phenotypic modification of Ki67 antigen‐negative G1‐ and G2‐phase cells in phorbol ester‐treated molt‐4 human leukemia cells

Tsurusawa, Masahito; Fujimoto, T

doi:10.1002/cyto.990200207

Cited by 15 publications

(9 citation statements)

References 18 publications

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“…No significant difference was observed according to the PCNA LI for patients treated with AHF cytometry. Tsurusawa and Fujimoto (1995) reported a down-regulation of Ki-67 antigen expression to an undetectable level in tumour cells with a relatively long G1 duration. In these conditions, Ki-67 LI may be measured lower than the true growth fraction.…”

Section: Discussionmentioning

confidence: 99%

Prognostic values of proliferating cell nuclear antigen (PCNA) and Ki-67 for radiotherapy of oesophageal squamous cell carcinomas

et al. 1999

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The relationship of immunohistochemical indices of proliferating cell nuclear antigen (PCNA) and Ki-67 to local control and survival rates for patients with oesophageal squamous cell carcinomas treated by definitive radiotherapy (RT) was investigated. Biopsy materials before RT were obtained from 65 patients with oesophageal cancer. The median PCNA labelling index (LI) and the median Ki-67 LI were 52% and 45% respectively. The PCNA LI was independent of known prognostic factors on local control for oesophageal cancer, although Ki-67 LI correlated with several prognostic factors. In the univariate analysis, patients with the PCNA LI of < 52% or the Ki-67 LI of < 45% showed significantly higher local recurrence rates than those with higher LIs (both P < 0.05). This difference in local control rate according to LIs was prominent for the patients treated with conventional fractionation. In the multivariate analysis, T-stage ( P = 0.0056) and PCNA LI ( P = 0.0332) were significant factors for local control in the final model using a stepwise regression procedure. In conclusion, PCNA LI and Ki-67 LI were significantly correlated with local control probabilities in oesophageal squamous cell carcinomas treated by definitive RT. © 1999 Cancer Research Campaign

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Section: Discussionmentioning

confidence: 99%

Prognostic values of proliferating cell nuclear antigen (PCNA) and Ki-67 for radiotherapy of oesophageal squamous cell carcinomas

et al. 1999

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“…The Ki-67int phenotype observed in this study may represent a similar population of unresponsive cells and it is intriguing that we also measured reduced frequencies of IL-2-producing CD4 + T cells. Another possibility is that Ki-67int cells are arrested in one stage of the cell cycle (36), or in a state of anergy, as previously described in the setting of “aborted activation” (37). …”

Section: Discussionmentioning

confidence: 99%

Exposure to SIV in utero results in reduced viral loads and altered responsiveness to postnatal challenge

et al. 2015

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HIV disease progression appears to be driven by high levels of immune activation. Given observations that fetal exposure to infectious pathogens in utero can result in reduced immune responses, or tolerance, to those pathogens postnatally, we hypothesized that fetal exposure to HIV may tolerize the fetus to the virus, thus reducing damage caused by immune activation if infected later in life. To test this hypothesis, fetal rhesus macaques (Macaca mulatta) were injected with the attenuated virus SIVmac1A11 in utero and then challenged with pathogenic SIVmac239 one year after birth. SIVmac1A11-injected animals had significantly reduced plasma RNA viral loads (p<0.02) up to 35 weeks post-infection. Generalized estimating equations (GEE) analysis was performed to identify immunologic and clinical measurements significantly associated with plasma RNA viral load. A positive association was observed with the proportion of CD8+ T cells expressing the transcription factor, FoxP3, and the proportion of CD4+ T cells producing the lymphoproliferative cytokine, IL-2, while an inverse relationship was found with the levels of circulating CD4+ and CD8+ T cells expressing intermediate levels of the proliferation marker, Ki-67. Further analyses demonstrated that animals exposed to SIV in utero appeared to have enhanced SIV-specific cellular immune responses, expressed lower levels of the exhaustion marker, PD-1, on CD8+ T cells, and had more circulating Th17 cells when compared to controls. While the development of tolerance was not demonstrated, these data suggest that rhesus monkeys exposed to SIVmac1A11 in utero had distinct immune responses associated with the control of viral replication after postnatal challenge.

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“…Lack of Ki67 antigenicity in early G 1 cells is a frequently described phenomenon [2,15,16]. Tsurusawa and Fujimoto [45] reported a down regulation of Ki67 antigen expression to an undetectable level in tumour cells with relatively long G 1 duration. Landberg and Roos [28] found that G 1 cells in the first cell cycle are Ki67 antigen negative, while continuously cycling cells express Ki67 antigen also in G 1.…”

Section: Pcna Versus Brdumentioning

confidence: 99%

Evaluation of proliferation parameters in in vivo bromodeoxyuridine labelled lung cancers

Tinnemans

Lenders

Ramaekers

et al. 1995

Vichows Archiv A Pathol Anat

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In a series of 44 bronchial biopsies from patients suspected of having endobronchial lung carcinoma, the validity of proliferating cell nuclear antigen (PCNA) and Ki67 antigen as proliferative indicators was evaluated in ethanol fixed, paraffin embedded tissue. The percentages of cells positive for these markers were compared to the in vivo bromodeoxyuridine (BrdU) labelling index. A good correlation was found between PCNA immunoreactivity and BrdU labelling index, while Ki67-antigen expression showed a significant relation with BrdU labelling index and with PCNA expression. All three parameters showed a trend towards similar values for the individual cases. Based on the fact that Ki67 antigen is expressed in all cycling cells, whereas replicon-associated PCNA and BrdU only reflect the S-phase fraction, the differences between Ki67-antigen scores on the one hand and BrdU and PCNA scores on the other were smaller than expected. In order to determine the degree of concordance between immunohistochemically and flow cytometrically detected proliferation variables, BrdU incorporation was measured using both methods in duplicate bronchial specimens. Discrepancies in labelling indices were observed predominantly in DNA diploid samples, with consistently lower values in the flow cytometrically analysed specimens. In tumour specimens with an aneuploid DNA content, flow cytometric determination of proliferative activity yielded results similar to those obtained by tissue section examination. We conclude that the scores for PCNA and Ki67 antigen, immunohistochemically detected in ethanol fixed, paraffin embedded tissue reflect functional proliferative activity.

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Cell cycle progression and phenotypic modification of Ki67 antigen‐negative G1‐ and G2‐phase cells in phorbol ester‐treated molt‐4 human leukemia cells

Cited by 15 publications

References 18 publications

Prognostic values of proliferating cell nuclear antigen (PCNA) and Ki-67 for radiotherapy of oesophageal squamous cell carcinomas

Prognostic values of proliferating cell nuclear antigen (PCNA) and Ki-67 for radiotherapy of oesophageal squamous cell carcinomas

Exposure to SIV in utero results in reduced viral loads and altered responsiveness to postnatal challenge

Evaluation of proliferation parameters in in vivo bromodeoxyuridine labelled lung cancers

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