Cell Cycle and Cancer

Mir, Manzoor Ahmad; Sofi, Shazia

doi:10.1007/978-981-19-8911-7_4

Cited by 3 publications

(5 citation statements)

References 105 publications

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“…Cells go through a sequence of activities known as the cell cycle as they multiply and divide. Cancer cells frequently have aberrant control over the cell cycle, which allows them to divide uncontrolled [165]. Cancer cell cycle analysis with the help of flow cytometry is possible.…”

Section: Applications Of Flow Cytometrymentioning

confidence: 99%

Flow cytometry: Aspects and application in plant and biological science

Jyoti,

Chandel,

Singh

2023

Journal of Biophotonics

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Flow cytometry is a potent method that enables the quick and concurrent investigation of several characteristics of single cells in solution. Photodiodes or photomultiplier tubes are employed to detect the dispersed and fluorescent light signals that are produced by the laser beam as it passes through the cells. Photodetectors transform the light signals produced by the laser into electrical impulses. A computer then analyses these electrical impulses to identify and measure the various cell populations depending on their fluorescence or light scattering characteristics. Based on their fluorescence or light scattering properties, cell populations can be examined and/or isolated. This review covers the basic principle, components, working and specific biological applications of flow cytometry, including studies on plant, cell and molecular biology and methods employed for data processing and interpretation as well as the potential future relevance of this methodology in light of retrospective analysis and recent advancements in flow cytometry.

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Section: Applications Of Flow Cytometrymentioning

confidence: 99%

Flow cytometry: Aspects and application in plant and biological science

Jyoti,

Chandel,

Singh

2023

Journal of Biophotonics

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“…To summarize, DNA replication and cell division are the last steps in a sequence of developmental events known as the cell cycle (Figure 2) that may be divided into four distinct phases: G1, S (synthesis), G2, and M [27]. Compound I was chosen for further examination based on the promising results from the MTT experiment to assess its influence on the apoptotic process and investigate its capacity to play an active role in cell cycle progression in HepG2 cells.…”

Section: Apoptotic Evaluationmentioning

confidence: 99%

“…Improved cancer outcomes are possible through the use of novel medications and tumor-specific delivery of FDA-approved anticancer treatments [4]. Some of the derivatives [indole-3-pyrazole-5-carboxamide analogues (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)] had anticancer effects against cancer cell lines that were comparable to or superior to those of sorafenib. With IC 50 values ranging from 0.6 to 2.9 M, compound 18 demonstrated strong activity against the HCC cell lines [5].…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Effects of Synthesis Novel Phenoxyacetamide Derivatives as Potent Apoptotic Inducer against HepG2 Cells through PARP-1 Inhibition

Sayed,

Nabil,

El-Shenawy

et al. 2023

Pharmaceuticals

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To discover potential cytotoxic agents, new semi-synthetic phenoxy acetamide derivatives, compound I and compound II, were synthesized, characterized, and screened for their cytotoxic activity against breast cancer (MCF-7) and liver cancer (HepG2) cell lines. The two compounds were more promising against HepG2 than the MCF-7 cell line according to IC50 values. When tested against the HepG2 cell line, compound I, and compound II both had significantly increased cytotoxic activity when compared to the reference medication 5-Fluorouracil (5-FU), with IC50 values of 1.43 M, 5.32 M, and 6.52 M for compound 1, 5-FU and compound II, respectively. Also, compound I displayed a degree of selectivity towards cancer cells compared to normal cells. Compound I significantly enhanced HepG2 total apoptotic cell death by about a 24.51-fold increase. According to cell cycle analysis, compound I induced the arrest of the cell cycle phases G1/S and blocked the progression of the HepG2 cells. Applying the RT-PCR technique achieved a highly significant upregulation in pro-apoptotic genes. The anti-apoptotic gene was significantly downregulated. There was an intrinsic and extrinsic pathway, but the intrinsic pathway was the dominant one. Tumor growth suppression as measured by tumor weight and volume and other hematological, biochemical, and histopathological analyses confirmed the efficacy of compound I as an anticancer agent in vivo examination. Finally, the molecular docking study revealed that compound I was properly docked inside the binding site of PARP-1 protein with stable binding energies and interactive binding modes. Therefore, compound I shows promise as a selective anti-cancer derivative for the treatment of liver cancer after more investigations and clinical studies. This selectivity is a favorable characteristic in the developing cytotoxic agents for cancer treatment, as it indicates a potential for reduced harm to health tissues.

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“…[1,2] Curiously, there are over 200 cancer forms. [3][4][5][6][7] Even though several therapies are practiced, the need for new anticancer agents is ever-increasing because of issues with the safety, efficacy, efficiency, bioavailability, and toxicity of old drugs such as cisplatin. [8][9][10][11] Although currently used anticancer drugs show remarkable effects during initial treatment, a large majority of patients develop resistance as treatment proceeds.…”

Section: Introductionmentioning

confidence: 99%

“…Despite many years of great effort by scientists and clinicians, cancer is the second leading cause of mortality worldwide [1,2] . Curiously, there are over 200 cancer forms [3–7] . Even though several therapies are practiced, the need for new anticancer agents is ever‐increasing because of issues with the safety, efficacy, efficiency, bioavailability, and toxicity of old drugs such as cisplatin [8–11] .…”

Section: Introductionmentioning

confidence: 99%

Cellular Mechanistic Considerations on Cytotoxic Mode of Action of Phosphino Ru(II) and Ir(III) Complexes

Wojtala,

Komarnicka,

Kyzioł

et al. 2023

Eur J Inorg Chem

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Two piano‐stool ruthenium(II) complexes Ru(η6‐p‐cymene)Cl2PPh2CH2OH (RuPOH) and Ru(η6‐p‐cymene)Cl2P(p‐OCH3Ph)2CH2OH (RuMPOH) and two half‐sandwich iridium(III) complexes Ir(η5‐Cp*)Cl2PPh2CH2OH (IrPOH) and Ir(η5‐Cp*)Cl2P(p‐OCH3Ph)2CH2OH (IrMPOH) have been studied in terms of potential anticancer activity on previously selected cell line (human lung adenocarcinoma). Based on experimental results obtained in monoculture in vitro model mechanistic considerations on the possible cellular modes of action have been carried out. ICP‐MS analysis revealed the higher cellular uptake for less hydrophobic Ir(III) complexes in comparison to the corresponding Ru(II) compounds. Cytometric analysis showed a predominance of apoptosis over the other types of cell death for all complexes. The apoptotic pathway was confirmed by a decrease in mitochondrial membrane potential and the activation of caspases‐3/9 for both Ru(II) and Ir(III) complexes. It was concluded that in the case of Ru(II) complexes the intense ROS generation is mainly responsible for the resulting cytotoxicity. The corresponding Ir(III) complexes trigger simultaneously at least three different cytotoxic pathways i.e., depletion of mitochondrial potential, activation of caspases‐dependent apoptosis, and ROS‐associated oxidation. Thus, it can be assumed that the final accumulation of toxic effects over time via parallel activation of different pathways results in the highest cytotoxicity in vitro exhibited by Ir(III) complexes when compared with Ru(II) complexes.

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Cell Cycle and Cancer

Cited by 3 publications

References 105 publications

Flow cytometry: Aspects and application in plant and biological science

Flow cytometry: Aspects and application in plant and biological science

In Vitro and In Vivo Effects of Synthesis Novel Phenoxyacetamide Derivatives as Potent Apoptotic Inducer against HepG2 Cells through PARP-1 Inhibition

Cellular Mechanistic Considerations on Cytotoxic Mode of Action of Phosphino Ru(II) and Ir(III) Complexes

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