Cell contact and cell adhesion during tissue organization

Saxén, Lauri; Wartiovaara, Jorma

doi:10.1002/ijc.2910010307

Cited by 60 publications

(15 citation statements)

References 28 publications

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“…Electron microscopy studies conducted by Saxen and Wartiovaara in the 1960s showed subapical vesicles in pretubular aggregates [32]. Because EG was observed in small puncta prior to lumen formation, we examined whether EG colocalized with Rab11, a known marker of recycling endosomes [33].…”

Section: Resultsmentioning

confidence: 99%

Role of CD34 family members in lumen formation in the developing kidney

Yang

Zimmerman

Tsunezumi

et al. 2016

Developmental Biology

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Previous studies have shown CD34 family member Podocalyxin is required for epithelial lumen formation in vitro. We demonstrate that Endoglycan, a CD34 family member with homology to Podocalyxin, is produced prior to lumen formation in developing nephrons. Endoglycan localizes to Rab11-containing vesicles in nephron progenitors, and then relocalizes to the apical surface as progenitors epithelialize. Once an apical/luminal surface is formed, Endoglycan (and the actin-binding protein Ezrin) localize to large, intraluminal structures that may be vesicles/exosomes. We generated mice lacking Endoglycan and found mutants had timely initiation of lumen formation and continuous lumens, similar to controls. Mice with conditional deletion of both Endoglycan and Podocalyxin in developing nephrons also had normal tubular lumens. Despite this, Endoglycan/Podocalyxin is required for apical recruitment of the adaptor protein NHERF1, but not Ezrin, in podocyte precursors, a subset of the epithelia. In summary, while CD34 family members appear dispensable for lumen formation, our data identify Endoglycan as a novel pre-luminal marker and suggest lumen formation occurs via vesicular trafficking of apical cargo that includes Endoglycan.

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Section: Resultsmentioning

confidence: 99%

Role of CD34 family members in lumen formation in the developing kidney

Yang

Zimmerman

Tsunezumi

et al. 2016

Developmental Biology

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“…The morphogenesis of the kidney tubules in vivo is triggered by the ingrowing ureter bud. It has been suggested that the triggering leads to a gradual increase in adhesiveness of the responding cells, and a morphogenetic field of determined cells 710 cell layers deep is assumed to form around the inductor tissue (6). So far, there are no molecular data to support this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

“…After the morphogenetically silent period the mesenchymal cells are soon aggregated into pretubular condensates. The trapping of cells into aggregates and their subsequent behavior seen in electron microscopy and time-lapse motion pictures suggest increased adhesiveness of the cells as a response to the induction (6). The molecular basis of the early aggregation phase, however, is still unknown.…”

mentioning

confidence: 99%

Induction of a basement membrane glycoprotein in embryonic kidney: possible role of laminin in morphogenesis.

Ekblom¹,

Alitalo²,

Vaheri³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

253

121

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The glycoprotein laminin is found exclusively in the basement membranes of adult tissues, not in the mesenchymal stroma. We studied the appearance and distribution of laminin during the early formation of kidney tubules in mouse embryos and in an in vitro transfilter model system. In immunofluorescence using affinity-purified antibodies, the distribution of laminin showed a clear correlation, both spatially and temporally, to the early stages of tubule formation. In vivo, laminin was first detected in a punctate pattern in areas where the pretubular aggregates form; later, it became confined to the basement membranes of the tubules. In experiments in vitro, the nephrogenic mesenchyme was found to form tubules after 12-24 hr of transfilter contact with the inductor. The first laminin spots were found after 12 hr of culture, 24 hr before overt morphogenesis. As the mesenchymal cells began to aggregate and elongate (at 36 hr), laminin was detected in those cells destined to become epithelial, and at 48 hr it was not found in cells remaining in the stroma. In more mature tubules (at 72 hr), laminin was seen as a sharp band in the basement membranes.

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“…A somewhat similar morphogenetic event occurs when new nephric tubules differentiate in an undifferentiated metanephric blastema (Saxen and Wartiovaara, 1966).…”

Section: Morphogenesis Of the Seminiferous Cordsmentioning

confidence: 97%

Sertoli cells and testicular differentiation in the rat fetus

Magre¹,

Jost²

1991

J. Elec. Microsc. Tech.

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The fetal testis is not merely a precursor of the adult organ: it is indeed an endocrine gland whose function is the masculinization of the fetus. It differs physiologically and morphologically from the adult testis. In this paper, the first stages of testicular differentiation in the rat are described, with special emphasis on the ultrastructural aspects. At the stage of 13.5 days after fertilization, the first Sertoli cells differentiate; they are characterized by a voluminous and little electron dense cytoplasm, a well-developed RER formed by vesicles and short cisternae filled with a flocculent material. Progressively, they polarize and adhere to one another by adherens-like junctions and cytoplasmic interdigitations to form the differentiating seminiferous cords. In the basal part of the Sertoli cells, a mat of microfilaments differentiates under the plasmalemma, while cytoplasmic blebs protruding in the extracellular space tend to disappear. A continuous basal lamina delineating the seminiferous cords begins to appear on day 14.5 and becomes widespread on day 15.5. These observations, when compared with other data from the literature, emphasize the fact that the differentiation of the Sertoli cells is the first morphological event during testicular differentiation. A possible role of the Sertoli cells in the subsequent organogenesis of the testis is suggested.

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Cell contact and cell adhesion during tissue organization

Cited by 60 publications

References 28 publications

Role of CD34 family members in lumen formation in the developing kidney

Role of CD34 family members in lumen formation in the developing kidney

Induction of a basement membrane glycoprotein in embryonic kidney: possible role of laminin in morphogenesis.

Sertoli cells and testicular differentiation in the rat fetus

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