Role of CD34 family members in lumen formation in the developing kidney

Yang, Zhufeng; Zimmerman, Susan E.; Tsunezumi, Jun; Braitsch, Caitlin M.; Trent, Cary; Bryant, David M.; Cleaver, Ondine; González-Manchón, Consuelo; Marciano, Denise K.

doi:10.1016/j.ydbio.2016.08.009

Cited by 15 publications

(19 citation statements)

References 43 publications

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“…For the purposes of this analysis, only expression in ECs was scored, although a few factors (E.g. Vegfr1, Claudin-5, Nrp1, Podxl1) also exhibited non-endothelial expression [38].…”

Section: Resultsmentioning

confidence: 99%

Spatiotemporal heterogeneity and patterning of developing renal blood vessels

et al. 2018

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The kidney vasculature facilitates the excretion of wastes, the dissemination of hormones, and the regulation of blood chemistry. To carry out these diverse functions, the vasculature is regionalized within the kidney and along the nephron. However, when and how endothelial regionalization occurs remains unknown. Here, we examine the developing kidney vasculature to assess its 3-dimensional structure and transcriptional heterogeneity. First, we observe that endothelial cells (ECs) grow coordinately with the kidney bud as early as E10.5, and begin to show signs of specification by E13.5 when the first arteries can be identified. We then focus on how ECs pattern and remodel with respect to the developing nephron and collecting duct epithelia. ECs circumscribe nephron progenitor populations at the distal tips of the ureteric bud (UB) tree and form stereotyped cruciform structures around each tip. Beginning at the renal vesicle (RV) stage, ECs form a continuous plexus around developing nephrons. The endothelial plexus envelops and elaborates with the maturing nephron, becoming preferentially enriched along the early distal tubule. Lastly, we perform transcriptional and immunofluorescent screens to characterize spatiotemporal heterogeneity in the kidney vasculature and identify novel regionally enriched genes. A better understanding of development of the kidney vasculature will help instruct engineering of properly vascularized ex vivo kidneys and evaluate diseased kidneys.

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“…For the purposes of this analysis, only expression in ECs was scored, although a few factors (E.g. Vegfr1, Claudin-5, Nrp1, Podxl1) also exhibited non-endothelial expression [38].…”

Section: Resultsmentioning

confidence: 99%

Spatiotemporal heterogeneity and patterning of developing renal blood vessels

et al. 2018

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“…As part of the CD34 family of transmembrane sialomucin proteins, its composition makes it both negatively charged and slippery due to glycosylation. Thus, Podocalyxin may help opposing apical membranes open by electrostatic repulsion and anti-adhesion 50,51 . In summary, the trafficking events mediating apical membrane establishment and lumen formation require an intricate and coordinated network of trafficking machinery, polarity complexes, and membrane proteins.…”

Section: Lumen Formation In Vitromentioning

confidence: 99%

Insane in the apical membrane: Trafficking events mediating apicobasal epithelial polarity during tube morphogenesis

Jewett

Prekeris

2018

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The creation of cellular tubes is one of the most vital developmental processes, resulting in the formation of most organ types. Cells have co-opted a number of different mechanisms for tube morphogenesis that vary among tissues and organisms; however, generation and maintenance of cell polarity is fundamental for successful lumenogenesis. Polarized membrane transport has emerged as a key driver not only for establishing individual epithelial cell polarity, but also for coordination of epithelial polarization during apical lumen formation and tissue morphogenesis. In recent years, much work has been dedicated to identifying membrane trafficking regulators required for lumenogenesis. In this review we will summarize the findings from the past couple of decades in defining the molecular machinery governing lumenogenesis both in 3D tissue culture models and during organ development in vivo.

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“…Nonparaffin sections were fixed in 4% PFA/PBS, permeabilized with 0.3% Triton X-100/PBS (PBST), and blocked with 10% donkey sera/PBST, as performed previously. 31 Sections were incubated with primary antibodies overnight (4°C), then with fluorophore-conjugated secondary antibodies, and mounted with Prolong Gold (Invitrogen). Immunohistochemistry was performed similarly except secondary antibodies were HRP-conjugated and DAB was used for staining.…”

Section: Immunofluorescence Histology and Rna In Situ Hybridizationmentioning

confidence: 99%

Nephronectin Regulates Mesangial Cell Adhesion and Behavior in Glomeruli

Zimmerman

Hiremath

Tsunezumi

et al. 2018

JASN

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A critical aspect of kidney function occurs at the glomerulus, the capillary network that filters the blood. The glomerular basement membrane (GBM) is a key component of filtration, yet our understanding of GBM interactions with mesangial cells, specialized pericytes that provide structural stability to glomeruli, is limited. We investigated the role of nephronectin (), a GBM component and known ligand of 81 integrin. Immunolocalization and hybridization studies in kidneys of adult mice revealed that nephronectin is produced by podocytes and deposited into the GBM. Conditional deletion of from nephron progenitors caused a pronounced increase in mesangial cell number and mesangial sclerosis. Nephronectin colocalized with 81 integrin to novel, specialized adhesion structures that occurred at sites of mesangial cell protrusion at the base of the capillary loops. Absence of nephronectin disrupted these adhesion structures, leading to mislocalization of 81. Podocyte-specific deletion of also led to mesangial sclerosis in mice. These results demonstrate a novel role for nephronectin and81 integrin in a newly described adhesion complex and begin to uncover the molecular interactions between the GBM and mesangial cells, which govern mesangial cell behavior and may have a role in pathologic states.

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Role of CD34 family members in lumen formation in the developing kidney

Cited by 15 publications

References 43 publications

Spatiotemporal heterogeneity and patterning of developing renal blood vessels

Spatiotemporal heterogeneity and patterning of developing renal blood vessels

Insane in the apical membrane: Trafficking events mediating apicobasal epithelial polarity during tube morphogenesis

Nephronectin Regulates Mesangial Cell Adhesion and Behavior in Glomeruli

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