Cell–cell interactions via non-covalent click chemistry

Abstract

Metabolic glycoengineering with unnatural sugars became a valuable tool for introducing recognition markers on the cell membranes via bioorthogonal chemistry. By using this strategy, we functionalized the surface of tumor...

A β-Cyclodextrin-Albumin Conjugate for Enhancing Therapeutic Efficacy of Cytotoxic Drugs

“…Under these circumstances, we designed the compound 1 that permits the attachment of three -CD units per albumin molecule, thereby enhancing the potential of the circulating protein to bind doxorubicin once functionalized by 1 in the blood stream. Thus, the derivative 1 was synthesized from the primary amine 4 45 already described in the literature via nucleophile substitution with the N-hydroxysuccinimide ester 5 (for the full synthesis of 4 see the supporting information).…”

Engineering unactivated platelets for targeted drug delivery

“…Techniques such as modifying azide groups or other markers on the cell surface allow for non-covalent click chemical reactions to induce cell adhesion. 77 Alternatively, membrane fusion can promote efficient combination between different types of cells. 78,79 It is feasible to modify cells in vitro to facilitate their interaction with target cells in vivo.…”

Metabolic Glycoengineering: A Promising Strategy to Remodel Microenvironments for Regenerative Therapy

“…But recently, bioorthogonal analogs ( Figure 2(b) Bb) are also being exploited to enhance artificial adhesion since the click chemistry could endow cells to be chemically connected, building the “functional cell complexes.” For example, Ac4ManNAz introduces azide groups into the cell surface as the first step; secondly, those azide groups are modified, respectively, with tetrazine (Tz) or trans -cyclooctene (TCO); thirdly, by mixing the modified cells, Tz-TCO click chemistry could produce intercellular adhesion [ 83 , 84 ]. Click reaction between cyclodextrins (CDs) and adamantly (Adam) was also used to artificially combine A459 lung tumor cells and Jurkat cells, then triggered the activation of NK cells, and leads to the death of cancer cells [ 85 ].…”