Cell-Cell Fusion Induced by Measles Virus Amplifies the Type I Interferon Response

Herschke, Florence; Plumet, Sébastien; Duhen, Thomas; Azocar, Olga; Druelle, Johan; Laine, David; Wild, T. F.; Rabourdin–Combe, Chantal; Gerlier, Denis; Valentin, Hélène

doi:10.1128/jvi.00078-07

Cited by 45 publications

(34 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This implies that membrane fusion is not a limiting factor in this experimental system. Syncytium formation is limited after experimental infection of monkeys (14,26,29), and extensive fusion can be detrimental because it ampli- fies the type I interferon response (18). Thus, wild-type MV may be under selective pressure to limit fusion; from this perspective, the generation of F-protein TM mutants with enhanced fusion function is more likely than if wild-type MV were highly fusogenic.…”

Section: Discussionmentioning

confidence: 99%

The Measles Virus Fusion Protein Transmembrane Region Modulates Availability of an Active Glycoprotein Complex and Fusion Efficiency

Mühlebach

Léonard

Cattaneo

2008

J Virol

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

The Measles Virus Fusion Protein Transmembrane Region Modulates Availability of an Active Glycoprotein Complex and Fusion Efficiency

Mühlebach

Léonard

Cattaneo

2008

J Virol

View full text Add to dashboard Cite

“…However, the mechanism by which the virus induces the formation of syncytia has not yet been elucidated. Possibly, fusion may be guided by the GaHV-1 virus through the interaction of envelope glycoproteins with cell membrane receptors, similarly to the infection by measles virus, thereby ensuring their replication and spread (Herschke et al, 2007).…”

Section: Pathological Immunohistochemical and Molecular Findings Inmentioning

confidence: 99%

Pathological, immunohistochemical, and molecular findings in commercial laying hens and in backyard chickens naturally infected with the infectious laryngotracheitis virus

Preis

Fiúza²,

Silva³

et al. 2014

Rev. Bras. Cienc. Avic.

View full text Add to dashboard Cite

KeywordsAvian infectious laryngotracheitis, conventional PCR, histopathology, immunohistochemistry, laying hen.Submitted: November/2013 Approved: June/2014 ABSTRACT Seventy-eight chickens from a very high poultry density (approximately eight million) region and twelve backyard chickens from neighboring areas were analyzed by histopathology and additional techniques for the presence of the infectious laryngotracheitis virus. The virus distribution was determined in different tissues using immunohistochemistry (IHC) and polymerase chain reaction (PCR). The disease was histopathologically diagnosed in 41.0% (32/78) of the commercial layers. Lesions were mainly characterized by syncytial cells with eosinophilic intranuclear inclusion body formed from the hyperplastic epithelium of the upper respiratory tract, primary and secondary bronchi, and conjunctiva. IHC showed 70% (21/30) positive signal in the larynx/trachea and, 53.8% (14/26) in the lungs, either in epithelial cells or syncytia. In the turbinates and paranasal sinuses, 29.6% (8/27) of samples showed positive signal. PCR detected the following gallid herpesvirus 1-positive percentages: conjunctiva 63.2% (31/49), lungs 57.6% (30/52), turbinates and paranasal sinuses 56% (28/50), and larynx/trachea 50% (39/78). IHC showed to be a useful additional tool for definitive ILT diagnosis, especially during the subacute phase of the disease when syncytial cells with intranuclear inclusion bodies are no longer observed. PCR using specific primers from ICP4 gene, generating a product of 237 base pairs, was sensitive for ILT diagnosis, and very useful for rapid detection of GaHV-1 in chickens. Fixed tissues allowing histopatological examination and detection of GaHV-1 by PCR, are a good option in areas where farms are located several hundred kilometers away from a diagnostic center, reducing problems with conservation of fresh samples and the risk of virus spread.

show abstract

“…CD209 and TLR2 do not serve as entry receptors but actively trigger (TLR2) or modulate (CD209) signaling pathways in DCs. In addition to their role in entry, CD150 and CD46 may also modulate signaling pathways in DCs attenuated rather than wild-type strains to replicate in DCs (Ohgimoto et al 2007), and the ability of induce formation of multinucleated giant cells in mature, but not immature DCs (and concomitantly, amplification of type I IFN production) was found restricted to a CD46-adapted strain (Herschke et al 2007). This is in contrast to earlier findings where wild-type MV (or recombinants expressing wild-type H protein) preferentially caused fusion in DCs (Ohgimoto et al 2001).…”

Section: Infection and DC Viability And Maturationmentioning

confidence: 95%

Measles Virus-Induced Immunosuppression

Schneider-Schaulies

Current Topics in Microbiology and Immunology

View full text Add to dashboard Cite

Immunosuppression is the major cause of infant death associated with acute measles and therefore of substantial clinical importance. Major hallmarks of this generalized modulation of immune functions are (1) lymphopenia, (2) a prolonged cytokine imbalance consistent with suppression of cellular immunity to secondary infections, and (3) silencing of peripheral blood lymphocytes, which cannot expand in response to ex vivo stimulation. Lymphopenia results from depletion, which can occur basically at any stage of lymphocyte development, and evidently, expression of the major MV receptor CD150 plays an important role in targeting these cells. Virus transfer to T cells is thought to be mediated by dendritic cells (DCs), which are considered central to the induction of T cell silencing and functional skewing. As a consequence of MV interaction, viability and functional differentiation of DCs and thereby their expression pattern of co-stimulatory molecules and soluble mediators are modulated. Moreover, MV proteins expressed by these cells actively silence T cells by interfering with signaling pathways essential for T cell activation.

show abstract

Cell-Cell Fusion Induced by Measles Virus Amplifies the Type I Interferon Response

Abstract: Measles virus (MeV) infection is characterized by the formation of multinuclear giant cells (MGC). We report that beta interferon (IFN-␤

Cited by 45 publications

References 84 publications

The Measles Virus Fusion Protein Transmembrane Region Modulates Availability of an Active Glycoprotein Complex and Fusion Efficiency

The Measles Virus Fusion Protein Transmembrane Region Modulates Availability of an Active Glycoprotein Complex and Fusion Efficiency

Pathological, immunohistochemical, and molecular findings in commercial laying hens and in backyard chickens naturally infected with the infectious laryngotracheitis virus

Measles Virus-Induced Immunosuppression

Contact Info

Product

Resources

About