Cell-binding peptides on the material surface guide stem cell fate of adhesion, proliferation and differentiation

Abstract

The binding of stem cells with a peptide-immobilized material surface, where several peptides are designed and conjugated on the material with multiple reactions, activates several signaling pathways that determine the stem cell fate.

Controlling differentiation of stem cells via bioactive disordered cues

“…9,36,63 Several parameters need to be taken into consideration, including the length of the linker between the carboxyl groups and cell-binding peptide moieties to ensure high accessibility of the peptide functional groups to the cell-surface receptors. 19–21,35,36,103,104…”

“…9,36,63 Several parameters need to be taken into consideration, including the length of the linker between the carboxyl groups and cell-binding peptide moieties to ensure high accessibility of the peptide functional groups to the cellsurface receptors. [19][20][21]35,36,103,104 Interesting findings have been reported regarding the differentiation of hMSCs into specialized phenotypes in response to microscale spatially distributed ligands. For example, Bilem et al 17,18 provided clear evidence that hMSCs perceive geometric cues in their microenvironment, it is quite intriguing that RGD/BMP-2 micropatterns shaped as triangles and squares significantly enhanced hMSC osteogenesis, while those shaped as rectangles exerted no specific effect on hMSC fate, as compared to homogeneous surfaces.…”

How Hydrogel Stiffness Affects Adipogenic Differentiation of Mesenchymal Stem Cells under Controlled Morphology

“…For the other polymer hydrogels such as polyacrylamide hydrogel, 15 poly-(ethylene glycol) hydrogel, 16 and poly(dimethylsiloxane) 17 that do not have specific cell interaction motifs, cell recognition motif-containing molecules such as Arg-Gly-Asp peptide, collagen, and fibronectin have been used to facilitate cell attachment. 18 Cells cultured on or in the hydrogels interact with the motifs and sense the stiffness of hydrogels. The hydrogels having cell recognition motifs can more closely mimic the extracellular microenvironments, while the results reflect the synergistic effects of both the cell recognition motifs and the hydrogel stiffness.…”

“…The hydrogels of extracellular matrices such as collagen and gelatin have integrin recognition motifs which enable cells to attach and spread. For the other polymer hydrogels such as polyacrylamide hydrogel, poly­(ethylene glycol) hydrogel, and poly­(dimethylsiloxane) that do not have specific cell interaction motifs, cell recognition motif-containing molecules such as Arg-Gly-Asp peptide, collagen, and fibronectin have been used to facilitate cell attachment . Cells cultured on or in the hydrogels interact with the motifs and sense the stiffness of hydrogels.…”

3D culture of bovine articular chondrocytes in viscous medium encapsulated in agarose hydrogels for investigation of viscosity influence on cell functions

“…1,2 Biochemical cues in the microenvironments have been recognized as important factors to affect cell functions for a very long time. 3,4 In recent decades, biophysical cues like elasticity and viscoelasticity also have drawn some attention as the solid tissues of our body exhibit these typical mechanical properties. [5][6][7] In vivo, in addition to solid tissues, some cells inhabit extracellular fluids such as mucus, bone marrow and synovial fluids.…”