Cell-Based Strain Remodeling of a Nonfibrous Matrix as an Organizing Principle for Vasculogenesis

Rüdiger, Daniel; Kick, Kerstin; Goychuk, Andriy; Vollmar, Angelika M.; Frey, Erwin; Zahler, Stefan

doi:10.1016/j.celrep.2020.108015

Cited by 19 publications

(44 citation statements)

References 50 publications

(53 reference statements)

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“…However, FNBP1 knockdown significantly decreased the invasive capacity of MKN1, indicating its lack of ability to penetrate the extracellular matrix (Figure 3D). Thus, ablation of FNBP1 selectively inhibited cellular invasion through a dense extracellular matrix [31,32].…”

Section: Ablation Of Fnbp1 Results In the Loss Of Invasion Capacitymentioning

confidence: 98%

Sp1-Induced FNBP1 Drives Rigorous 3D Cell Motility in EMT-Type Gastric Cancer Cells

Yoon

Hwang

Chun

et al. 2021

IJMS

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Cancer is heterogeneous among patients, requiring a thorough understanding of molecular subtypes and the establishment of therapeutic strategies based on its behavior. Gastric cancer (GC) is adenocarcinoma with marked heterogeneity leading to different prognoses. As an effort, we previously identified a stem-like subtype, which is prone to metastasis, with the worst prognosis. Here, we propose FNBP1 as a key to high-level cell motility, present only in aggressive GC cells. FNBP1 is also up-regulated in both the GS subtype from the TCGA project and the EMT subtype from the ACRG study, which include high portions of diffuse histologic type. Ablation of FNBP1 in the EMT-type GC cell line brought changes in the cell periphery in transcriptomic analysis. Indeed, loss of FNBP1 resulted in the loss of invasive ability, especially in a three-dimensional culture system. Live imaging indicated active movement of actin in FNBP1-overexpressed cells cultured in an extracellular matrix dome. To find the transcription factor which drives FNBP1 expression in an EMT-type GC cell line, the FNBP1 promoter region and DNA binding motifs were analyzed. Interestingly, the Sp1 motif was abundant in the promoter, and pharmacological inhibition and knockdown of Sp1 down-regulated FNBP1 promoter activity and the transcription level, respectively. Taken together, our results propose Sp1-driven FNBP1 as a key molecule explaining aggressiveness in EMT-type GC cells.

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Section: Ablation Of Fnbp1 Results In the Loss Of Invasion Capacitymentioning

confidence: 98%

Sp1-Induced FNBP1 Drives Rigorous 3D Cell Motility in EMT-Type Gastric Cancer Cells

Yoon

Hwang

Chun

et al. 2021

IJMS

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“…Similar to what was described above for adhesion—the type of ECM molecules [ 19 , 21 , 45 ] and topography [ 29 ] also affect extent and mode of migration. The blocking of integrin binding sites for laminin causes a morphology switch from elongated to round with blebs [ 45 ].…”

Section: Cell Migrationmentioning

confidence: 88%

“…Different ECM proteins or binding sites differentially affect adhesion [ 18 ], spreading [ 18 , 19 ], migration [ 18 , 19 ], and proliferation of endothelial cells [ 20 ]. Their presence is a prerequisite for a certain cell behavior (e.g., the presence of laminin is necessary for tube formation [ 17 , 20 , 21 ]). Individual proteins can have positive effects on angiogenesis, such as the enhancement of sprouting by collagen IV [ 22 ], or negative effects, such as the disruption of tube formation by collagen I [ 21 , 23 ].…”

Section: Cell-matrix Contactsmentioning

confidence: 99%

“…Their presence is a prerequisite for a certain cell behavior (e.g., the presence of laminin is necessary for tube formation [ 17 , 20 , 21 ]). Individual proteins can have positive effects on angiogenesis, such as the enhancement of sprouting by collagen IV [ 22 ], or negative effects, such as the disruption of tube formation by collagen I [ 21 , 23 ]. Furthermore, different ECM proteins in combination with the respective integrin binding partner affect intracellular signaling pathways and cell behavior.…”

Section: Cell-matrix Contactsmentioning

confidence: 99%

“…The blocking of integrin binding sites for laminin causes a morphology switch from elongated to round with blebs [ 45 ]. Increased migration is associated with collagen I [ 21 , 45 ]. In this latter case, cells align along the collagen network [ 46 , 47 ] and follow the stiffer collagen fibers [ 21 ].…”

Section: Cell Migrationmentioning

confidence: 99%

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Mechanical Aspects of Angiogenesis

Kretschmer

Rüdiger

Zahler

2021

Cancers

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Angiogenesis is of high clinical relevance as it plays a crucial role in physiological (e.g., tissue regeneration) and pathological processes (e.g., tumor growth). Besides chemical signals, such as VEGF, the relationship between cells and the extracellular matrix (ECM) can influence endothelial cell behavior during angiogenesis. Previously, in terms of the connection between angiogenesis and mechanical factors, researchers have focused on shear forces due to blood flow. However, it is becoming increasingly important to include the direct influence of the ECM on biological processes, such as angiogenesis. In this context, we focus on the stiffness of the surrounding ECM and the adhesion of cells to the ECM. Furthermore, we highlight the mechanical cues during the main stages of angiogenesis: cell migration, tip and stalk cells, and vessel stabilization. It becomes clear that the different stages of angiogenesis require various chemical and mechanical cues to be modulated by/modulate the stiffness of the ECM. Thus, changes of the ECM during tumor growth represent additional potential dysregulations of angiogenesis in addition to erroneous biochemical signals. This awareness could be the basis of therapeutic approaches to counteract specific processes in tumor angiogenesis.

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Hylozoic by Design: Converging Material and Biological Complexities for Cell‐Driven Living Materials with 4D Behaviors

Shariati

Ling

Fuchs

et al. 2021

Adv Funct Materials

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The emergence of novel techniques in biology and material science, coupled with greater understandings of cell-material interactions, have given rise to the creation of living materials and bioarchitectures imbued with engineered living behaviors. These multidimensional materials and constructs capable of performing programmable and time-or stimuli-dependent activities, namely 4D behaviors, may do so as a result of material features alone, or as a product of incorporated biological or cellular agents. This review discusses fabrication strategies employed in the development of 4D living materials and examples of their 4D activities driven by cellular events or processes. The fabrication strategies investigated throughout are focused on those that facilitate the responsive, functional transformation of the 3D structure with the time that extends beyond changes driven solely by material features, and enable an increase in the cell-dependent functional capacity of the construct. Living materials comprised of cells and their own products, as well as both cells and added non-living materials are investigated. Further, the authors analyze how material complexities and biological complexities have thus far been interfaced to allow for intricate living behaviors, and discuss the design considerations and challenges encountered in developing living materials. Finally, the future directions of living materials are outlined.

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Cell-Based Strain Remodeling of a Nonfibrous Matrix as an Organizing Principle for Vasculogenesis

Cited by 19 publications

References 50 publications

Sp1-Induced FNBP1 Drives Rigorous 3D Cell Motility in EMT-Type Gastric Cancer Cells

Sp1-Induced FNBP1 Drives Rigorous 3D Cell Motility in EMT-Type Gastric Cancer Cells

Mechanical Aspects of Angiogenesis

Hylozoic by Design: Converging Material and Biological Complexities for Cell‐Driven Living Materials with 4D Behaviors

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