Cell Attachment and Spreading on Carbon Nanotubes Is Facilitated by Integrin Binding

Imaninezhad, Mozhdeh; Schober, Joseph; Griggs, David W.; Ruminski, Peter; Kuljanishvili, Irma; Zustiak, Silviya Petrova

doi:10.3389/fbioe.2018.00129

Cited by 28 publications

(20 citation statements)

References 35 publications

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“…In contrast, Figures 5C,D show significantly higher fluorescent intensities across the PEDOT:PSS/MWCNT hybrid scaffolds in addition to localized “hotspots.” These hotspots can be attributed to the presence of CNT domains in the scaffolds, as evidenced by SEM images and Raman mapping described in section Morphology and Structure. As CNTs have already been demonstrated to improve neurite outgrowth and projection in neural tissue engineering (Hu et al, 2004; Lovat et al, 2005), we assume that the presence of such “hotspots” could possibly provide favorable sites for cell attachment and adhesion, as well as for guiding cell growth (Hirata et al, 2012; Imaninezhad et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

3D Hybrid Scaffolds Based on PEDOT:PSS/MWCNT Composites

Jayaram

Pitsalidis

et al. 2019

Front. Chem.

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Conducting polymer scaffolds combine the soft-porous structures of scaffolds with the electrical properties of conducting polymers. In most cases, such functional systems are developed by combining an insulating scaffold matrix with electrically conducting materials in a 3D hybrid network. However, issues arising from the poor electronic properties of such hybrid systems, hinder their application in many areas. This work reports on the design of a 3D electroactive scaffold, which is free of an insulating matrix. These 3D polymer constructs comprise of a water soluble conducting polymer (PEDOT:PSS) and multi-walled carbon nanotubes (MWCNTs). The insertion of the MWCNTs in the 3D polymer matrix directly contributes to the electron transport efficiency, resulting in a 7-fold decrease in resistivity values. The distribution of CNTs, as characterized by SEM and Raman spectroscopy, further define the micro- and nano-structural topography while providing active sites for protein attachment, thereby rendering the system suitable for biological/sensing applications. The resulting scaffolds, combine high porosity, mechanical stability and excellent conducting properties, thus can be suitable for a variety of applications ranging from tissue engineering and biomedical devices to (bio-) energy storage.

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Section: Resultsmentioning

confidence: 99%

3D Hybrid Scaffolds Based on PEDOT:PSS/MWCNT Composites

Jayaram

Pitsalidis

et al. 2019

Front. Chem.

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“…Polyacrylonitrile-based fibers have a more complex structure and consist of many tubular elements combined into a three-dimensional structure. Such a structure can and does provide high strength due to the overlap of tubular elements, but its high stiffness is difficult to preserve solely by in-creasing structure density and decreasing the total pore volume [ 23 , 24 ]. Figure 3 shows the stress–strain curve of PAN nanofibers and reveals thatthe tensile modulus of the polymeric nanofiber membrane without HA modification is equal to 0.408 ± 0.09 MPa.…”

Section: Resultsmentioning

confidence: 99%

In-Vitro Cytotoxicity Study: Cell Viability and Cell Morphology of Carbon Nanofibrous Scaffold/Hydroxyapatite Nanocomposites

et al. 2021

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Electrospun carbon nanofibers (CNFs), which were modified with hydroxyapatite, were fabricated to be used as a substrate for bone cell proliferation. The CNFs were derived from electrospun polyacrylonitrile (PAN) nanofibers after two steps of heat treatment: stabilization and carbonization. Carbon nanofibrous (CNF)/hydroxyapatite (HA) nanocomposites were prepared by two different methods; one of them being modification during electrospinning (CNF-8HA) and the second method being hydrothermal modification after carbonization (CNF-8HA; hydrothermally) to be used as a platform for bone tissue engineering. The biological investigations were performed using in-vitro cell counting, WST cell viability and cell morphology after three and seven days. L929 mouse fibroblasts were found to be more viable on the hydrothermally-modified CNF scaffolds than on the unmodified CNF scaffolds. The biological characterizations of the synthesized CNF/HA nanofibrous composites indicated higher capability of bone regeneration.

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“…Interestingly, PLA-CaP added with FBS components showed a more than twofold increase in cell content after 7 days of incubation compared to untreated PLA, and was statistically higher than PLA + FBS, also presenting evidence of increased interactions with human osteoblast spheroids, as shown by fluorescence microscopy. The incorporation of serum proteins such as vitronectin and fibronectin may be a way to increase the number of bioactive sites that are recognized by cells on the surface of materials such as PLA [ 86 ].…”

Section: Discussionmentioning

confidence: 99%

Biomimetic Mineralization on 3D Printed PLA Scaffolds: On the Response of Human Primary Osteoblasts Spheroids and In Vivo Implantation

et al. 2020

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This study aimed to assess the response of 3D printed polylactic acid (PLA) scaffolds biomimetically coated with apatite on human primary osteoblast (HOb) spheroids and evaluate the biological response to its association with Bone Morphogenetic Protein 2 (rhBMP-2) in rat calvaria. PLA scaffolds were produced via 3D printing, soaked in simulated body fluid (SBF) solution to promote apatite deposition, and characterized by physical-chemical, morphological, and mechanical properties. PLA-CaP scaffolds with interconnected porous and mechanical properties suitable for bone repairing were produced with reproducibility. The in vitro biological response was assessed with human primary osteoblast spheroids. Increased cell adhesion and the rise of in vitro release of growth factors (Platelet-Derived Growth Factor (PDGF), Basic Fibroblast Growth Factor (bFGF), Vascular Endothelial Growth Factor (VEGF) was observed for PLA-CaP scaffolds, when pre-treated with fetal bovine serum (FBS). This pre-treatment with FBS was done in a way to enhance the adsorption of serum proteins, increasing the number of bioactive sites on the surface of scaffolds, and to partially mimic in vivo interactions. The in vivo analysis was conducted through the implantation of 3D printed PLA scaffolds either alone, coated with apatite (PLA-CaP) or PLA-CaP loaded with rhBMP-2 on critical-sized defects (8 mm) of rat calvaria. PLA-CaP+rhBMP2 presented higher values of newly formed bone (NFB) than other groups at all in vivo experimental periods (p < 0.05), attaining 44.85% of NFB after six months. These findings indicated two new potential candidates as alternatives to autogenous bone grafts for long-term treatment: (i) 3D-printed PLA-CaP scaffold associated with spheroids, since it can reduce the time of repair in situ by expression of biomolecules and growth factors; and (ii) 3D-printed PLA-CaP functionalized rhBMP2 scaffold, a biocompatible, bioactive biomaterial, with osteoconductivity and osteoinductivity.

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Cell Attachment and Spreading on Carbon Nanotubes Is Facilitated by Integrin Binding

Cited by 28 publications

References 35 publications

3D Hybrid Scaffolds Based on PEDOT:PSS/MWCNT Composites

3D Hybrid Scaffolds Based on PEDOT:PSS/MWCNT Composites

In-Vitro Cytotoxicity Study: Cell Viability and Cell Morphology of Carbon Nanofibrous Scaffold/Hydroxyapatite Nanocomposites

Biomimetic Mineralization on 3D Printed PLA Scaffolds: On the Response of Human Primary Osteoblasts Spheroids and In Vivo Implantation

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