Cell architecture–cell function dependencies on titanium arrays with regular geometry

Matschegewski, Claudia; Staehlke, Susanne; Loeffler, Ronny; Lange, Regina; Chai, Feng; Kern, D. P.; Beck, Ulrich; Nebe, Barbara

doi:10.1016/j.biomaterials.2010.03.073

Cited by 90 publications

(98 citation statements)

References 58 publications

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“…Studies in vitro support these findings with a different behavior of cells in dependence on the scale of the topography. Cells are able to sense the micro-and nanoscale topography and react with bridging of grooves or conforming the surface structure [7][8][9][10]. An attractive approach to generate nanostructures on titanium is the formation of nanotubes [11,12].…”

Section: Cell Regulation By Physical Characteristics Of a Materials Tomentioning

confidence: 99%

Cell-material interaction

Rychly

Nebe

2013

BioNanoMaterials

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Abstract:The interaction of cells with material surfaces is of fundamental relevance and contributes to the clinical success of implants. Cells sense their physico-chemical environment resulting in focal adhesion reorganization, spatio-temporal localization and activation of integrin dependent signalling proteins as well as phenotypic changes, e.g., of the actin cytoskeleton. The technology of designing instructive biomaterials involves chemical modifications by grafting of chemical groups, adhesion ligands and growth factors. Physical modifications of the materials are created by structuring the surfaces stochastically and geometrically and by modifications of the material stiffness. Insights into the mechanism at the interface that are involved in the regulation of cells such as stem cells, osteoblasts and chondrocytes by materials will advance the development of innovative biomaterials in regenerative medicine.

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Section: Cell Regulation By Physical Characteristics Of a Materials Tomentioning

confidence: 99%

Cell-material interaction

Rychly

Nebe

2013

BioNanoMaterials

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“…It is discussed in the literature that interaction of integrins (adhesion receptors which also act in the focal complexes like vinculin) with the extracellular matrix occurs first on a scale of 100 nm (¼0.1 mm). [32] Concerning topography in the micrometer-scale we could present recently that actin re-organization in one cell's dimension intensively occurs on cubic pillar structures with sizes of 3-5 mm [33] but not on smaller sizes of these geometrical structures (unpublished). Thus, we assume for our results that there exist strong chemical influencing factors such as positive surface charges as produced by physical plasma polymers [11] which are dominant over the pico-nano-topography.…”

Section: Initial Spreading Is Enhanced On Positively Charged Surfacesmentioning

confidence: 99%

Positively Charged Material Surfaces Generated by Plasma Polymerized Allylamine Enhance Vinculin Mobility in Vital Human Osteoblastss

et al. 2010

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Several studies suggest that the modification of an implant surface by chemical means plays an important role in bone tissue engineering. Previously we have shown that osteoblast cell adhesion and spreading can strongly be increased by a positively charged surface. Cell adhesion and migration are two vital processes that are completely dependent on coordinated formation of focal adhesions. Changes in the organization of the actin cytoskeleton and the focal adhesions are essential for numerous cellular processes including cell motility and tissue morphogenesis. We examined the mobility of the cytoskeletally associated protein vinculin on functionalized surfaces using plasma polymerized allylamine (PPAAm), a homogenous plasma polymer layer with randomly distributed amino groups. In living, GFP–vinculin transfected osteoblastic cells we determined a significant increase in vinculin mobility and vinculin contact length on PPAAm compared to collagen I coated surfaces during the initial adhesion phase. We suggest that positive charges control the cell physiology which seems to be dominant over the integrin receptor binding to collagen I. The results emphasize the role of the surface charge for the design of artificial scaffolds in bone repair.

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“…42 Vinculin is a membrane-bound protein involved in FA, acting as a linker between integrins and actin cytoskeleton. 43 Figures 5 and S3 as well as Figures 6 and S4 show vinculin-actin-nucleus and FAK-actin-nucleus tricolors staining fluorescence images of cells on the coatings after 24 hours of incubation, respectively. In the absence of serum (Figures 5 and 6), the FA contact, as visualized by FA-recruited vinculin and FAK, are distributed over the peripheral and central regions of cells on the NG, S67, and S96 coatings, but almost no FA contact can be seen in cells on the S137 coating.…”

Section: Focal Adhesion Contact and Cytoskeleton Organizationmentioning

confidence: 99%

“…In the literature, emerging data demonstrate that surface topographies modulate integrin and in turn mediate downstream behavior of cells, such as adhesion, proliferation, and differentiation; 23,24,[40][41][42][43][44][45][46][47][48][49] however, the mechanism by which topography affects integrin is not clear. Although the cell adhesion result, reported by Lim et al, performing on nanoscale pits in the presence of serum, suggested that nanotopography affects cell behavior via a direct mechanism, they cannot rule out the possibility that serum protein adsorption on nanotopographies may play a role in nanotopography regulation of cell behavior.…”

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confidence: 99%

Direct role of interrod spacing in mediating cell adhesion on Sr-HA nanorod-patterned coatings

Zhou¹,

Han²,

Lu³

2014

IJN

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The process in which nanostructured surfaces mediate cell adhesion is not well understood, and may be indirect (via protein adsorption) or direct. We prepared Sr-doped hydroxyapatite (Sr 1 -HA) 3D nanorods (with interrod spacing of 67.3±3.8, 95.7±4.2, and 136.8±8.7 nm) and 2D nanogranulate patterned coatings on titanium. Employing the coatings under the same surface chemistry and roughness, we investigated the indirect/direct role of Sr 1 -HA nanotopographies in the regulation of osteoblast adhesion in both serum-free and serum-containing Dulbecco’s Modified Eagle/Ham’s Medium. The results reveal that the number of adherent cells, cell-secreted anchoring proteins (fibronectin, vitronectin, and collagen), vinculin and focal adhesion kinase (FAK) denoted focal adhesion (FA) contact, and gene expression of vinculin, FAK, and integrin subunits (α 2 , α 5 , α v , β 1 , and β 3 ), undergo significant changes in the inter-nanorod spacing and dimensionality of Sr 1 -HA nanotopographies in the absence of serum; they are significantly enhanced on the <96 nm spaced nanorods and more pronounced with decreasing interrod spacing. However, they are inhibited on the >96 nm spaced nanorods compared to nanogranulated 2D topography. Although the adsorption of fibronectin and vitronectin from serum are higher on 136.8±8.7 nm spaced nanorod patterned topography than nanogranulated topography, cell adhesion is inhibited on the former compared to the latter in the presence of serum, further suggesting that reduced cell adhesion is independent of protein adsorption. It is clearly indicated that 3D nanotopography can directly modulate cell adhesion by regulating integrins, which subsequently mediate anchoring proteins’ secretion and FA formation rather than via protein adsorption.

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Cell architecture–cell function dependencies on titanium arrays with regular geometry

Cited by 90 publications

References 58 publications

Cell-material interaction

Cell-material interaction

Positively Charged Material Surfaces Generated by Plasma Polymerized Allylamine Enhance Vinculin Mobility in Vital Human Osteoblastss

Direct role of interrod spacing in mediating cell adhesion on Sr-HA nanorod-patterned coatings

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