Ceftazidime-hydrolysing CTX-M-15 extended-spectrum beta-lactamase (ESBL) in Poland

Baraniak, Anna

doi:10.1093/jac/dkf151

Cited by 105 publications

(64 citation statements)

References 6 publications

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“…We observed that CTX-M was the most common ESBL type in Norwegian E. coli (90%) isolates. This is consistent with the emergence of CTX-M-producing E. coli strains worldwide (2,5,7,8,9,28).…”

Section: Discussionsupporting

confidence: 86%

Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-β-Lactamase-Producing Clinical Isolates ofEscherichia coliandKlebsiella pneumoniaein Norway

Tofteland¹,

Haldorsen²,

Hegstad³

et al. 2007

J Clin Microbiol

127

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Consecutive clinical isolates of Escherichia coli (n ‫؍‬87Systemic infections with extended-spectrum-␤-lactamase (ESBL)-producing Enterobacteriaceae are associated with severe adverse clinical outcomes (7,12,25). It is thus essential for a diagnostic microbiology laboratory to have updated methods for the detection of ESBL-producing strains, taking into account the local epidemiology of ESBL genotypes and their various expression profiles. As very little is known about ESBL genotypes in Norway, we designed a study for the detection and characterization of ESBL production in clinical isolates of Escherichia coli and Klebsiella pneumoniae with reduced susceptibilities to oxyimino-cephalosporins from routine diagnostic samples. More specifically, we examined (i) the abilities of different phenotypic methods to detect ESBL-producing strains in relation to MICs of oxyimino-cephalosporins, (ii) the molecular basis for ESBL production by typing of the most prevalent ␤-lactamase genes (bla TEM , bla SHV , and bla CTX-M ) and the relationships between MIC profiles for oxyimino-cephalosporins and different bla groups, and (iii) the occurrence of multiple-antibiotic resistance.(The results of this study were presented in part at the European Congress of Clinical Microbiology and Infectious Diseases, Prague, Czech Republic, 2004.) MATERIALS AND METHODSStudy design. Consecutive nonduplicate isolates of E. coli and K. pneumoniae with reduced susceptibilities to oxyimino-cephalosporins (MIC Ͼ 1 mg/liter) were collected in 18 of 24 Norwegian diagnostic microbiology laboratories covering

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Section: Discussionsupporting

confidence: 86%

Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-β-Lactamase-Producing Clinical Isolates ofEscherichia coliandKlebsiella pneumoniaein Norway

Tofteland¹,

Haldorsen²,

Hegstad³

et al. 2007

J Clin Microbiol

127

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“…However, there has been a recent report of CTX-M-15 exhibiting high enzyme activity against ceftazidime as well as cefotaxime [19]. The isolate described in this study similarly exhibited a high-level resistance to cefotaxime (MIC, >256 μ g/mL), ceftazidime (MIC, 64 μ g/mL), and aztreonam (MIC, >256 μ g/mL), as determined by E tests.…”

Section: Discussionmentioning

confidence: 53%

Case Report of Pediatric Gastroenteritis Due to CTX-M-15 Extended-Spectrum β-Lactamase-Producing Salmonella enterica Serotype Enteritidis

et al. 2009

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“…The relatedness of the increase and the finding that bla CTX-M-14 was located on plasmids need further investigation. The remaining sporadic ESBLs were only rarely reported in other countries (2,6,11,19). Due at least in part to the busy international travel between Taiwan and foreign countries, we now see the presence of these rare ESBLs in Taiwan.…”

Section: Discussionmentioning

confidence: 77%

Development of a Multiplex PCR and SHV Melting-Curve Mutation Detection System for Detection of Some SHV and CTX-M β-Lactamases of Escherichia coli , Klebsiella pneumoniae , and Enterobacter cloacae in Taiwan

Chia

Chu

et al. 2005

J Clin Microbiol

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Infection by extended-spectrum ␤-lactamase (ESBL)-producing Enterobacteriaceae has been increasing in Taiwan. Accurate identification of the ESBL genes is necessary for surveillance and for epidemiological studies of the mode of transmission in the hospital setting. We describe herein the development of a novel system, which consists of a multiplex PCR to identify bla SHV , bla CTX-M-3 -like, and bla CTX-M-14 -like genes and a modified SHV melting-curve mutation detection method to rapidly distinguish six prevalent bla SHV genes (bla SHV-1 , bla SHV-2 , bla SHV-2a , bla SHV-5 , bla SHV-11 , and bla SHV-12 ) in Taiwan. Sixty-five clinical isolates, which had been characterized by nucleotide sequencing of the bla SHV and bla CTX-M genes, were identified by the system. The system was then used to genotype the ESBLs from 199 clinical isolates, including 40 Enterobacter cloacae, 68 Escherichia coli, and 91 Klebsiella pneumoniae, collected between August 2002 and March 2003. SHV-12 (80 isolates) was the most prevalent type of ESBL identified, followed in order of frequency by CTX-M-3 (65 isolates) and CTX-M-14 (36 isolates). Seventeen (9%) of the 199 clinical isolates harbored both SHV-and CTX-M-type ESBLs. In contrast toEnterobacter cloacae, the majority of which produced SHV-type ESBLs, E. coli and K. pneumoniae were more likely to possess CTX-M-type ESBLs. Three rare CTX-M types were identified through sequencing of the bla CTX-M-3 -like (CTX-M-15) and bla CTX-M-14 -like (CTX-M-9 and CTX-M-13) genes. The system appears to provide an efficient differentiation of ESBLs among E. coli, K. pneumoniae, and Enterobacter cloacae in Taiwan. Moreover, the design of the system can be easily adapted for similar purposes in areas where different ESBLs are prevalent.

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Ceftazidime-hydrolysing CTX-M-15 extended-spectrum beta-lactamase (ESBL) in Poland

Cited by 105 publications

References 6 publications

Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-β-Lactamase-Producing Clinical Isolates ofEscherichia coliandKlebsiella pneumoniaein Norway

Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-β-Lactamase-Producing Clinical Isolates ofEscherichia coliandKlebsiella pneumoniaein Norway

Case Report of Pediatric Gastroenteritis Due to CTX-M-15 Extended-Spectrum β-Lactamase-Producing Salmonella enterica Serotype Enteritidis

Development of a Multiplex PCR and SHV Melting-Curve Mutation Detection System for Detection of Some SHV and CTX-M β-Lactamases of Escherichia coli , Klebsiella pneumoniae , and Enterobacter cloacae in Taiwan

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