Cefotaxime-resistant E. coli in dairy and beef cattle farms—Joint analyses of two cross-sectional investigations in Germany

Hille, Katja; Ruddat, Inga; Schmid, A.; Hering, Johanna; Hartmann, Maria; Münchhausen, Christiane von; Schneider, B.; Messelhäußer, Ute; Friese, Anika; Mansfeld, Rolf; Käsbohrer, Annemarie; Hörmansdorfer, Stefan; Roesler, Uwe; Kreienbrock, Lothar

doi:10.1016/j.prevetmed.2017.05.003

Cited by 37 publications

(47 citation statements)

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“…; Hille et al . ), the study results suggest that herd‐ and sample‐level estimates of CTX‐M E. coli in beef cattle in GB were much lower. While the methodology of isolation of ESBL‐producing and AmpC phenotype E. coli in these two studies were not identical to those used in this study, they were similar in that they did involve a pre‐enrichment stage and then plating onto selective agar (MacConkey agar plates containing 1 mg l −1 cefotaxime).…”

Section: Discussionmentioning

confidence: 77%

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Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015

et al. 2019

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Aims: This study investigated the occurrence and genetic diversity of Enterobacteriaceae with extended-spectrum b-lactamase (ESBL)-, AmpC-and carbapenemase-mediated resistance in British beef cattle, and related risk factors. Methods and Results: Faecal samples (n = 776) were obtained from farms in England and Wales (n = 20) and Scotland (n = 20) in 2015. Isolates from selective agars were identified by MALDI ToF mass spectrometry. Selected isolates were characterized by multiplex PCR (bla CTX-M, bla OXA, bla SHV and bla TEM genes), whole-genome sequencing (WGS), minimum inhibitory concentrations and pulsed-field gel electrophoresis. None of the faecal samples yielded carbapenem-resistant Escherichia coli. Ten (25%) of the farms tested positive for ESBL-producing CTX-M Enterobacteriaceae, 15 (37Á5%) of the farms were positive for AmpC phenotype E. coli and none were positive for carbapenem-resistant E. coli. WGS showed a total of 30 different resistance genes associated with E. coli, Citrobacter and Serratia from ESBL agars, and colocation of resistance genes with bla CTX-M1 . Buying bulls and bringing in fattening cattle from another farm were identified as significant risk factors for positive samples harbouring CTX-M Enterobacteriaceae or AmpC phenotype E. coli respectively. Conclusions: Beef cattle on a proportion of farms in GB carry ESBLproducing Enterobacteriaceae. Factors, such as operating as a closed herd, may have an important role in reducing introduction and transmission of resistant Enterobacteriaceae. The results indicate management factors may play an important role in impacting ESBL prevalence. In particular, further study would be valuable to understand the impact of maintaining a closed herd on reducing the introduction of resistant Enterobacteriaceae. Significance and Impact of the Study: This is the first study showing the presence of ESBL-producing Enterobacteriaceae in British beef cattle.

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Section: Discussionmentioning

confidence: 77%

“…; Hille et al . ). In the United Kingdom, the first case of CTX‐M ESBL‐producing E. coli was reported in calves in a dairy herd in 2004 (Teale et al .…”

Section: Introductionmentioning

confidence: 97%

Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015

et al. 2019

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“…FU provided 50 CMY-2-producing E. coli isolates from chicken ( n = 9 in 2011 and n = 28 in 2012), turkey ( n = 1 in 2012 and n = 3 in 2014), pig ( n = 6 in 2011 and n = 2 in 2012) and cattle ( n = 1 in 2012) for WGS analysis. These isolates were collected in longitudinal- and cross-sectional studies in different livestock farms during the years 2011–2014 [ 66 – 70 ]; only isolates from unique production sites were included. In addition, BfR provided 63 CMY-2-producing E. coli isolates that were identified from 2500 food samples of different origins and matrices, collected and investigated by different German state laboratories, in the scope of a cross-sectional study on ESBL- E. coli in foodstuff in Germany (unpublished data).…”

Section: Methodsmentioning

confidence: 99%

Whole genome analyses of CMY-2-producing Escherichia coli isolates from humans, animals and food in Germany

et al. 2018

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BackgroundResistance to 3rd-generation cephalosporins in Escherichia coli is mostly mediated by extended-spectrum beta-lactamases (ESBLs) or AmpC beta-lactamases. Besides overexpression of the species-specific chromosomal ampC gene, acquisition of plasmid-encoded ampC genes, e.g. blaCMY-2, has been described worldwide in E. coli from humans and animals. To investigate a possible transmission of blaCMY-2 along the food production chain, we conducted a next-generation sequencing (NGS)-based analysis of 164 CMY-2-producing E. coli isolates from humans, livestock animals and foodstuff from Germany.ResultsThe data of the 164 sequenced isolates revealed 59 different sequence types (STs); the most prevalent ones were ST38 (n = 19), ST131 (n = 16) and ST117 (n = 13). Two STs were present in all reservoirs: ST131 (human n = 8; food n = 2; animal n = 6) and ST38 (human n = 3; animal n = 9; food n = 7). All but one CMY-2-producing ST131 isolates belonged to the clade B (fimH22) that differed substantially from the worldwide dominant CTX-M-15-producing clonal lineage ST131-O25b clade C (fimH30). Plasmid replicon types IncI1 (n = 61) and IncK (n = 72) were identified for the majority of blaCMY-2-carrying plasmids. Plasmid sequence comparisons showed a remarkable sequence identity, especially for IncK plasmids. Associations of replicon types and distinct STs were shown for IncK and ST57, ST429 and ST38 as well as for IncI1 and ST58. Additional β-lactamase genes (blaTEM, blaCTX-M, blaOXA, blaSHV) were detected in 50% of the isolates, and twelve E. coli from chicken and retail chicken meat carried the colistin resistance gene mcr-1.ConclusionWe found isolates of distinct E. coli clonal lineages (ST131 and ST38) in all three reservoirs. However, a direct clonal relationship of isolates from food animals and humans was only noticeable for a few cases. The CMY-2-producing E. coli-ST131 represents a clonal lineage different from the CTX-M-15-producing ST131-O25b cluster. Apart from the STdriven spread, plasmid-mediated spread, especially via IncI1 and IncK plasmids, likely plays an important role for emergence and transmission of blaCMY-2 between animals and humans.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4976-3) contains supplementary material, which is available to authorized users.

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“…Die Raten lagen bei den Mastitisproben deutlich höher als bei den Isolaten aus Tankmilchproben und denen von Mastrindern. In gezielten Untersuchungen wurde in den letzten Jahren wiederholt gezeigt, dass cephalosporinresistente E. coli häufig in deutschen Milch-und Mastrindbetrieben vorkommen [25] [26]. Aus Verbraucherschutzgesichtspunkten ist vorteilhaft, dass Mastitismilch nicht als Lebensmittel in den Verkehr gebracht werden darf und dass Milch, die als Lebensmittel in Verkehr gebracht wird, ganz überwiegend einer Wärmebehandlung unterliegt, wodurch etwaige mikrobielle Belastungen eliminiert werden.…”

Section: Entwicklung Der Resistenzraten Im Zeitverlaufunclassified

Antibiotikaresistenz von E. coli aus Rinderpopulationen in Deutschland

Tenhagen

Käsbohrer

Grobbel

et al. 2020

Tierarztl Prax Ausg G Grosstiere Nutztiere

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Zusammenfassung Gegenstand und Ziel Ziel dieser Arbeit ist, die Resistenzsituation bei Escherichia (E.) coli aus verschiedenen Rinderpopulationen zu beschreiben. Material und Methoden Dazu wurden die minimalen Hemmkonzentrationen (MHK) von Antibiotika gegen sowohl klinische als auch nicht klinische E. coli-Isolate von Milchkühen, Mastrindern, Mastkälbern und Kälbern ausgewertet. Diese Untersuchungen erfolgten im Rahmen des Monitoringprogramms GERM-Vet (klinische Isolate) sowie im Rahmen des Zoonosen-Monitorings (nicht klinische Isolate) mithilfe der Bouillon-Mikrodilutionsmethode. Zur einheitlichen Bewertung der ermittelten MHK dienten die vom European Centre for Antimicrobial Susceptibility Testing (EUCAST) veröffentlichten epidemiologischen Grenzwerte. Ergebnisse In die Untersuchung wurden insgesamt 5127 Isolate einbezogen. Die höchsten Resistenzraten (RR) gegenüber den meisten Substanzen ergaben sich bei Isolaten von erkrankten Kälbern, gefolgt von solchen von Mastkälbern und Jungrindern unter 1 Jahr am Schlachthof. Die niedrigsten RR wiesen E. coli-Isolate aus Tankmilchproben von Milchviehbetrieben und von Mastrindern im Bestand auf. Die Resistenzraten bei Mastitisisolaten waren deutlich höher als bei den nicht klinischen Isolaten aus Tankmilch, aber niedriger als bei den Isolaten von Kälbern und Jungrindern. Schlussfolgerungen und klinische Relevanz Vor allem die RR gegenüber den besonders wichtigen Substanzklassen Cephalosporine der 3. Generation und Fluorchinolone lagen bei Kälbern mit Enteritis, aber auch bei Isolaten aus Mastitisproben höher als in anderen Tierpopulationen. Ein Zusammenhang mit dem relativ hohen Einsatz dieser Substanzen bei Milchkühen ist naheliegend, da die Kälber über die Vertränkung nicht vermarktungsfähiger Milch sowohl gegenüber Arzneimittelrückständen als auch gegenüber resistenten Bakterien exponiert sind. Der Einsatz dieser Substanzklassen in der Rinderhaltung muss auf ein Minimum reduziert werden, um die weitere Ausbreitung dieser Resistenzen gegen diese Substanzen in der Rinderhaltung einzudämmen.

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Cefotaxime-resistant E. coli in dairy and beef cattle farms—Joint analyses of two cross-sectional investigations in Germany

Cited by 37 publications

References 25 publications

Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015

Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015

Whole genome analyses of CMY-2-producing Escherichia coli isolates from humans, animals and food in Germany

Antibiotikaresistenz von E. coli aus Rinderpopulationen in Deutschland

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